1. Complete Lipooligosaccharide Structure from Pseudoalteromonas nigrifaciens Sq02-Rifr and Study of Its Immunomodulatory Activity
- Author
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Rossella Di Guida, Angela Casillo, Antonietta Stellavato, Celeste Di Meo, Soichiro Kawai, Jun Kawamoto, Takuya Ogawa, Tatsuo Kurihara, Chiara Schiraldi, and Maria Michela Corsaro
- Subjects
cold-adapted ,endotoxin ,TLR-4 ,LPS ,structural characterization ,NMR ,Biology (General) ,QH301-705.5 - Abstract
Lipopolysaccharides (LPS) are surface glycoconjugates embedded in the external leaflet of the outer membrane (OM) of the Gram-negative bacteria. They consist of three regions: lipid A, core oligosaccharide (OS), and O-specific polysaccharide or O-antigen. Lipid A is the glycolipid endotoxin domain that anchors the LPS molecule to the OM, and therefore, its chemical structure is crucial in the maintenance of membrane integrity in the Gram-negative bacteria. In this paper, we reported the characterization of the lipid A and OS structures from Pseudoalteromonas nigrifaciens Sq02-Rifr, which is a psychrotrophic Gram-negative bacterium isolated from the intestine of Seriola quinqueradiata. The immunomodulatory activity of both LPS and lipid A was also examined.
- Published
- 2021
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