Search

Your search keyword '"Sanders, Andrew J."' showing total 10 results
Start Over Author "Sanders, Andrew J." Publisher mdpi ag
10 results on '"Sanders, Andrew J."'

5. Silencing CTNND1 Mediates Triple-Negative Breast Cancer Bone Metastasis via Upregulating CXCR4/CXCL12 Axis and Neutrophils Infiltration in Bone

Author

Lin, Qun, primary, Fang, Xiaolin, additional, Liang, Gehao, additional, Luo, Qing, additional, Cen, Yinghuan, additional, Shi, Yu, additional, Jia, Shijie, additional, Li, Juanmei, additional, Yang, Wenqian, additional, Sanders, Andrew J., additional, Gong, Chang, additional, and Jiang, Wenguo, additional

Published
2021
Full Text
View/download PDF

10. Expression of Hepatocyte Growth Factor-Like Protein in Human Wound Tissue and Its Biological Functionality in Human Keratinocytes.

Author

Glasbey JC, Sanders AJ, Bosanquet DC, Ruge F, Harding KG, and Jiang WG

Abstract

Hepatocyte growth factor-like protein (HGFl) and its receptor, Recepteur d'Origine Nantais (RON), have been implicated in the development of wound chronicity. HGFl and RON expression was detected in acute wound tissue, chronic wound tissue and in normal skin using quantitative polymerase chain reaction (Q-PCR). HGFl and RON expression was also assessed in chronic healing and chronic non-healing wound tissues using Q-PCR and immunohistochemical staining. Expression was similarly detected in the HaCaT immortalized human keratinocyte cell line using reverse transcription polymerase chain reaction (RT-PCR). rhHGFl was used to assess the impact of this molecule on HaCaT cell functionality using in vitro growth assays and electric cell-substrate impendence sensing (ECIS) migration assays. HGFl and RON transcript expression were significantly increased in acute wound tissue compared to chronic wound tissue and were also elevated, though non-significantly, in comparison to normal skin. Minimal expression was seen in both healing and non-healing chronic wounds. Treatment of HaCaT cells with rhHGFl had no effect on growth rates but did enhance cell migration. This effect was abolished by the addition of a phospholipase C gamma (PLCγ) small molecule inhibitor. The increased expression of HGFl and RON in acute, healing wounds and the pro-migratory effect of HGFl in an in vitro human keratinocyte model, may indicate a role for HGFl in active wound healing.

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results