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Dark-field microscopy offers several advantages, including high image contrast, minimal cell damage, and the absence of photobleaching of nanoprobes, which make it highly advantageous for cell imaging. The NIR-II window has emerged as a prominent research focus in optical imaging in recent years, with its low autofluorescence background in biological samples and high imaging SBR. In this study, we initially compared dark-field imaging results of colorectal cancer cells in both visible and NIR-II wavelengths, confirming the superior performance of NIR-II imaging. Subsequently, we synthesized gold nanorods with localized surface plasmon resonance (LSPR) absorption peaks in the NIR-II window. After bio-compatible modification, we non-specifically labeled colorectal cancer cells for NIR-II dark-field scattering imaging. The imaging results revealed a sixfold increase in SBR, especially in the 1425–1475 nm wavelength range. Finally, we applied this imaging system to perform dark-field imaging of cell nuclei in the NIR-II region and used GNRs for specific nuclear labeling in colorectal cancer cells. The resulting images exhibited higher SBR than non-specifically-labeled cell imaging, and the probe’s labeling was precise, confirming the potential application of this system in photothermal therapy and drug delivery for cancer cells.

Due to global warming and urbanization, high-temperature events—which frequently occur in cities—are presenting an increasing threat to the daily lives of human beings. In coastal cities, sea breezes can cool the near surface and improve the urban environment to some extent. Understanding the cooling characteristics of sea breeze on the urban environment is informative for improving and mitigating the urban heat island (UHI) effect. In this paper, we analyze the basic characteristics of the timing of the cooling effect of sea breeze in urban summer based on the long-term multi-point measurements of air temperatures. Additionally, the Weather Research and Forecasting (WRF) model is used to show the influence of sea breeze on cities in terms of the cooling action time. The whole process is reproduced based on a time distribution map created using the results of the WRF simulation. The measured temperature and WRF simulation results are also evaluated with observations. The results show little difference between the two. The analysis of the distribution map shows that the sea breeze gradually penetrates inland from coastal areas. It can therefore be concluded that the sea breeze blows at different speeds in different areas. Our results show that the sea breeze is weak in places near the coast, while it is significantly stronger around inland rivers. Moreover, in urban areas that are far from inland rivers, the speed of the sea breeze is evenly distributed in space. The spatial pattern of sea breeze retreat time and arrival time is reversed: retreats happen earlier in inland areas. The duration of the sea breeze shows a significantly decreasing trend from the coast to inland, with the longest duration at the southern end of the urban area near the coast.

Hot-band absorption (HBA)-induced anti-Stokes fluorescence (ASF) with longer-wavelength excitation is one effective pathway to deep penetration and low autofluorescence in intravital fluorescence imaging, raising demands for fluorophores with broad spectra, high absorption, and strong emission. However, typical fluorescent dyes display some emission quenching when their concentration is increased in order to obtain brighter fluorescence. In this work, the HBA-induced ASF of aggregation-induced emission (AIE) dots is reported. BPN-BBTD dots were synthesized and confirmed with a fluorescence enhancement and a considerable ASF intensity. In addition, the mechanism of ASF and the HBA process of BPN-BBTD dots were carefully validated and discussed. To obtain the full advantages of the long-wavelength excitation and the short fluorescence lifetime in deep-tissue bioimaging, a large-depth ASF confocal microscopic imaging of in vivo cerebral vasculature was conducted under the excitation of a 980 nm continuous wave laser after intravenous injection of BPN-BBTD dots. Meanwhile, the 3D structure of the cerebrovascular network was successfully reconstructed.