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Background: ST-elevation myocardial infarction (STEMI) and Takotsubo syndrome (TS) are two distinct cardiac conditions that both result in sudden loss of cardiac dysfunction and that are difficult to distinguish clinically. This study compared plasma protein changes in 24 women with STEMI and 12 women with TS in the acute phase (days 0–3 post symptom onset) and the stabilization phase (days 7, 14, and 30) to examine the molecular differences between these conditions. Methods: Plasma proteins from STEMI and TS patients were extracted during the acute and stabilization phases and analyzed via quantitative proteomics. Differential expression and functional significance were assessed. Data are accessible on ProteomeXchange, ID PXD051367. Results: During the acute phase, STEMI patients showed higher levels of myocardial inflammation and tissue damage proteins compared to TS patients, along with reduced tissue repair and anti-inflammatory proteins. In the stabilization phase, STEMI patients exhibited ongoing inflammation and disrupted lipid metabolism. Notably, ADIPOQ was consistently downregulated in STEMI patients in both phases. When comparing the acute to the stabilization phase, STEMI patients showed increased inflammatory proteins and decreased structural proteins. Conversely, TS patients showed increased proteins involved in inflammation and the regulatory response to counter excessive inflammation. Consistent protein changes between the acute and stabilization phases in both conditions, such as SAA2, CRP, SAA1, LBP, FGL1, AGT, MAN1A1, APOA4, COMP, and PCOLCE, suggest shared underlying pathophysiological mechanisms. Conclusions: This study presents protein changes in women with STEMI or TS and identifies ADIPOQ, SAA2, CRP, SAA1, LBP, FGL1, AGT, MAN1A1, APOA4, COMP, and PCOLCE as candidates for further exploration in both therapeutic and diagnostic contexts.

Increasing the planting density of mango orchards appears promising for obtaining higher yields, particularly during the first productive years. However, the challenge is to maintain a good balance between vegetative growth and fruit production in the longer term. The objective of this study was to decipher the effects of planting density, training system and cultivar on young mango trees’ growth and production. The experiment, conducted in North Queensland, consisted of five combinations of planting density and training system applied to the cultivars Keitt, Calypso and NMBP-1243. The planting densities were low (208 tree ha−1), medium (416 tree ha−1) and high (1250 tree ha−1). The closed vase conventional training system was applied at each density. Single leader and espalier on trellis training systems were applied at medium and high densities, respectively. The tree canopy dimensions were measured every 6 months from planting, and tree production was recorded from the third to the fifth years after planting. Vegetative growth and fruit production were the results of complex interactions between planting density, training system, cultivar and/or time. The expected increase in orchard yield with higher planting density was observed from the first productive year, despite lower individual tree production at high planting density. Lower vegetative growth and fruit production at high planting density were probably caused by competition between trees. NMBP-1243 and Keitt showed more rapid vegetative growth. Keitt was the most productive cultivar during the first three productive years. The detailed results of this study provide avenues to further explore the behaviour of mango trees at high planting densities.

Net ecosystem productivity (NEP) plays an important role in the quantitative evaluation of carbon source/sinks in terrestrial ecosystems. This study used Theil–Sen median trend analysis, the Mann–Kendall method, and the Geodetector model to analyze the variation patterns and its dominant factors of NEP from 2010 to 2020. The results showed the following: (1) During 2010–2020, the spatial distribution of carbon sinks in China’s terrestrial ecosystems showed a pattern of high in the southeast and low in the northwest. The area with NEP < 0 accounted for 44.74% of the total area, while the area with NEP > 0 accounted for 55.26%. (2) The northwest region belonged to the significant carbon source, while the other regions belonged to significant carbon sinks. (3) The annual average NEP in different sub-regions showed an increasing trend. During 2010–2020, the overall NEP in China showed a trend in shifting from low-level to high-level, indicating that the NEP of terrestrial ecosystems in China increased during the past 11 years. (4) The NEP gravity center in Northeast China showed a trend in moving southward and then northward, while that of the NEP gravity center in East China showed a circular migration trend of ‘northwest-southwest–northeast–southeast’. The gravity center of NEP in Northwest China was moving northeastward. The migration trajectory of the NEP gravity center in Southwest China presented a “Z” shape. The change in the gravity center of NEP in the central and southern regions had a strong circuitous nature, and the overall trend was to migrate southward. (5) The combined actions of climate change and human activities were the main reason for the change in NEP in China’s terrestrial ecosystem from 2010 to 2020. (6) There were obvious differences in the dominant driving factors of NEP evolution in different regions and different periods in the past 40 years.

Diatoms of the genus Pseudo-nitzschia are producers of the neurotoxin domoic acid that causes serious damage to marine fauna and aquaculture farms. These microalgae are known as the most abundant group of toxic phytoplankton in Peter the Great Bay, Northwestern Sea of Japan, during the bloom season, which, as was previously reported for members of this group in the study region, lasts in the autumn months. Autumnal blooms of toxic diatoms Pseudo-nitzschia spp. were studied in the period from 2017 to 2022 in Ussuri Bay, the largest body of water in Peter the Great Bay, which harbors numerous recreational area and aquaculture farms. As a result, the following changes in the composition of bloom-forming species were recorded: blooms were caused by the Pseudo-nitzschia delicatissima group in the period from 2017 to 2020 and by P. multistriata in 2021–2022. An assumption has been made that one of the factors responsible for blooms of P. multistriata, known as one of the most widespread species in Asian warm-water areas, is an abnormally high water temperature in the autumn of 2021 in the study area.

Basal stem rot (BSR) disease of oil palm (Elaeis guineensis Jacq.) spreads through the contact of the plant roots with Ganoderma boninense (G. boninense) Pat. inoculum in the soil. The soil properties can be altered by growing seedlings with or without G. boninense inoculum. In the early stage of infection, the symptoms are difficult to detect. Therefore, an understanding of the environmental soil conditions of the plant is crucial in order to indicate the presence of the fungus. This paper presents an analysis of the temporal changes of the soil properties associated with the G. boninense infection in oil palm seedlings. A total of 40 seedlings aged five months were used in the study, comprising 20 inoculated (infected seedlings: IS) and 20 control (healthy seedlings: HS) seedlings. The seedlings were grown in a greenhouse for six months (24 weeks) under a controlled environmental temperature and humidity. The data of the soil moisture content (MC in %), electrical conductivity (EC in µS/cm), and temperature (T in °C) for each seedling were collected daily using three MEC10 soil sensors every hour and then transferred to the ThingSpeak cloud using a 3G Internet connection. Based on the results, the mean MC and EC showed a decreasing trend, while the mean T showed an increasing trend in both HS and IS during the six-month monitoring period. The overall mean in both the monthly and weekly analysis of MC, EC, and T was higher in HS than IS. However, in the monthly analysis, a Student’s t-test at a 5% significance level showed that only the soil MC and EC were significantly different between HS and IS, while in the weekly analysis, HS was significantly different from IS in all parameters. This study suggests that soil MC, EC, and T can be used as indicators of the G. boninense infection, especially for the weekly data.

The public bicycle system is an important component of “mobility as a service” and has become increasingly popular in recent years. To provide a better understanding of the station activity and driving mechanisms of public bicycle systems, the study mainly compares the usage and temporal characteristics of public bicycles in the vicinity of the most common commuting-related points of interest and land use. It applies the peak hour factor, distribution fitting, and K-means clustering analysis on station-based data and performs the public bicycles usage and operation comparison among different points of interest and land use. The following results are acquired: (1) the demand type for universities and hospitals in peaks is return-oriented when that of middle schools is hire-oriented; (2) bike hire and return at metro stations and hospitals are frequent, while only the rental at malls is; (3) compared to middle schools and subway stations with the shortest bike usage duration, malls have the longest duration, valued at 18.08 min; and (4) medical and transportation land, with the most obvious morning return peak and the most concentrated usage in a whole day, respectively, both present a lag relation between bike rental and return. In rental-return similarity, the commercial and office lands present the highest level.

The composition of human breast milk changes in the first two months of life, adapting itself to the evolving needs of the growing new-born. Lipids in milk are a source of energy, essential fatty acids (FA), fat-soluble vitamins, and vital bioactive components. Information on breast milk FA of Malaysian lactating women is scarce. Based on convenience sampling, a total of 20 Malay breastfeeding women who fulfilled the inclusion criteria were recruited. Breast milk was collected three times from each subject at consecutive intervals of 2–3 weeks apart. A total of 60 breast milk samples were collected and classified into “transitional milk” (n = 8), “early milk” (n = 26) and “mature milk” (n = 26). All milk samples were air freighted to University of Groningen, Netherlands for analysis. The dominant breast milk FA were oleic acid, constituting 33% of total fatty acids, followed by palmitic acid (26%). Both these FA and the essential FA, linoleic acid (10%) and alpha-linolenic acid (0.4%), showed no significant changes from transitional to mature milk. Breast milk ratio of n-6:n-3 polyunsaturated fatty acids (PUFA) was comparatively high, exceeding 10 throughout the lactation period, suggesting a healthier balance of PUFA intake is needed in pregnancy and at postpartum.

Traditional villages are important carriers of traditional cultural heritage, and they have strong historical, cultural, aesthetic and tourism value for all countries and the international community. In China, the number of traditional villages is currently decreasing each year, and the precious material and non-material heritage is at risk of disappearing in the process of urbanization. A comprehensive understanding of the spatiotemporal patterns of traditional villages on multiple scales has important significance in protecting traditional culture, revitalizing traditional villages and achieving sustainable urbanization. Therefore, the spatiotemporal characteristics of traditional villages at the city, province, and geographic zone scales are explored by a series of Geographic Information System(GIS)-based methods in this article. Specifically, the analysis units are multi-scale, the applied methods are multi-variate, and the identified patterns are multi-perspective. The results demonstrate that the distribution of traditional villages in China is unbalanced over space and time. Moreover, the different spatiotemporal distributions of traditional villages are sensitive to scales. These findings clarify differences in the corresponding geographic and environmental factors, the level of economic development and local policy support. We further suggest that exploring the effective and suitable modes of protection and rural development is necessary. The results of this article revealing the unbalanced spatiotemporal distribution of traditional villages can provide valuable suggestions and insights into alleviating regional inequality in China.

Thirteen pit lakes of the Muskau Arch (Western Poland) were studied in the summer season of 2016. The lakes display great geomorphological and hydrochemical diversity. The aim of the study was to characterize temporal changes in the physicochemical conditions of the pit lakes over the last 30 years (1986–2016) and to determine the main factors influencing their evolution. All the lakes were characterized in terms of the same set of physicochemical parameters measured and used for comparative analysis with literature data from the years 1986 and 1992. The results showed significant temporal differentiation in terms of morphometric features and most of the physicochemical characteristics. pH, EC, Ca2+, Mg2+ and total Fe were among the most significantly differentiating parameters. A similar pattern of changes was found for most of the lakes, suggesting natural fluctuations. Despite the differences in values of thermal and oxygen profiles, there were also similarities in lake stratification. Additionally, meromictic conditions were found in 4 lakes. In some of the lakes a negative effect of neutralization and fertilization of the water was observed. Our results showed that the Muskau Arch lakes are subject to dynamic hydrogeological and hydrochemical changes. In addition to natural changes, human-caused transformations were among the key factors responsible for the differentiation of the physicochemical conditions.

Many statistical methods have been developed and used over time to analyze historical changes in hydrological time series, given the socioeconomic consequences of the changes in the water cycle components. The classical statistical methods, however, rely on many assumptions on the time series to be examined such as the normality, temporal and spatial independency and the constancy of the data distribution over time. When the assumptions are not fulfilled by the data, test results are not reliable. One way to relax these cumbersome assumptions and credibilize the results of statistical approaches is to make a combined use of graphical and statistical methods. To this end, two graphical methods of the refined cumulative sum of the difference between exceedance and non-exceedance counts of data points (CSD) and innovative trend analyses (ITA)-change boxes alongside the classical statistical Mann−Kendall (MK) method are used to analyze historical precipitation changes at 16 stations during 1960−2015 in the Black Sea region of Turkey. The results show a good match between the results of the graphical and statistical methods. The graphical CSD and ITA methods, however, are able to identify the hidden trends in the precipitation time series that cannot be detected using the statistical MK method.

Advances in the diagnosis and treatment of pulmonary hypertension (PH) have gradually improved the disease course. This retrospective cohort study aims to explore the diagnostic hemodynamic profile and survival of PH patients and their temporal changes, as well as investigate potential prognostic factors. Overall, 257 adult patients were diagnosed with PH following right heart catheterization (RHC) from January 2008 to June 2023 according to the hemodynamic cut-off values proposed by the corresponding ESC/ERS guidelines at the time RHC was performed. Of these patients, 46.3% were Group 1, 17.8% Group 2, 14.0% Group 3, 18.0% Group 4, and 3.0% Group 5 PH. Temporal improvement in both diagnostic hemodynamic profile and survival of patients with PH and pulmonary arterial hypertension (PAH) was identified after 2013. Survival analysis demonstrated 5-year survival rates of 65% in Group 1 PH (90.3% in idiopathic PAH) and 77% in Group 4 PH. PAH patients being at low risk at diagnosis presented a similar 1-year all-cause mortality rate (12.4%) with high-risk ones (12.8%), primarily due to non-PH-related causes of death (62%), while high-risk patients died mostly due to PH (67%). The observed improvements in diagnostic hemodynamic profiles and overall survival highlight the importance of timely diagnosis and successful treatment strategies in PH.

Seed fungal endophytes play a crucial role in assisting the overall health and success of their host plant; however, little is known about the factors that influence the diversity and composition of these endophytes, particularly with respect to how they change over time and within urban environments. Using culturing techniques, morphological analyses, and Sanger sequencing, we identified the culturable seed fungal endophytes of Banksia ericifolia at two urban and two natural sites in Sydney, New South Wales, Australia. A total of 27 Operational Taxonomic Units were obtained from 1200 seeds. Older cones were found to contain, on average, more colonised endophytes than younger cones. Species richness was also significantly influenced by cone age, with older cones being more speciose. Between urban and natural sites, the overall community composition did not change, although species richness and diversity were greatest at urban sites. Understanding how these endophytes vary in time and space may help provide an insight into the transmission pathways used and the potential role they play within the development and survival of the seed. This knowledge may also be crucial for restoration purposes, especially regarding the need to consider endophyte viability in ex situ seed collection and storage in seed-banking practices., Competing Interests: The authors declare no conflicts of interest.