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Rhododendron, a globally popular ornamental flower, is nevertheless limited in our understanding of the mechanisms underlying its fragrance formation. Notably, terpenoids are the most prevalent volatile metabolite produced by plants. In this study, gas chromatography–mass spectrometry (GC–MS), liquid chromatography–mass spectrometry (LC–MS) and transcriptomics sequencing were conducted to analyze the synthesis mechanisms of terpenoid fragrance compounds of petals in fragrant R. fortunei Lindl. (YJ) and non-fragrant R. “Nova Zembla” (NW). The results identified that (-)-myrtenol, linalool, pinene, myrtenyl acetate, and terpineol were key floral aroma substances in YJ. Furthermore, an analysis of KEGG enrichment and differentially expressed genes (DEGs) revealed that the bud and decay stages exhibited the highest number of enriched DEGs among different aroma types, indicating these as critical stages for the synthesis of terpenoid floral compounds. In this study, a structural gene, denoted as RfFDPS, was identified as a negative regulatory gene for monoterpene accumulation and a positive regulatory gene for sesquiterpene accumulation in YJ. Utilizing subcellular localization technology, we determined that RfFDPS proteins are located in the cytoplasm. A functional analysis through transient expression and gene silencing of RfFDPS demonstrated its ability to regulate the accumulation of monoterpenes and sesquiterpenes. The overexpression of RfFDPS led to an increase in the expression of structural genes related to terpenoid synthesis, resulting in a decrease in monoterpenes and an increase in sesquiterpenes. Conversely, gene silencing had the opposite effect. In conclusion, RfFDPS plays a pivotal role in the synthesis and release of terpenoid volatile compounds in YJ petals, laying a solid theoretical foundation for the cultivation and enhancement of aromatic R. species.

Plant structure has a large influence on crop yield formation, with branching and plant height being the important factors that make it up. We identified a gene, MtTCP18, encoding a TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factor highly conserved with Arabidopsis gene BRC1 (BRANCHED1) in Medicago truncatula. Sequence analysis revealed that MtTCP18 included a conserved basic helix–loop–helix (BHLH) motif and R domain. Expression analysis showed that MtTCP18 was expressed in all organs examined, with relatively higher expression in pods and axillary buds. Subcellular localization analysis showed that MtTCP18 was localized in the nucleus and exhibited transcriptional activation activity. These results supported its role as a transcription factor. Meanwhile, we identified a homozygous mutant line (NF14875) with a mutation caused by Tnt1 insertion into MtTCP18. Mutant analysis showed that the mutation of MtTCP18 altered plant structure, with increased plant height and branch number. Moreover, we found that the expression of auxin early response genes was modulated in the mutant. Therefore, MtTCP18 may be a promising candidate gene for breeders to optimize plant structure for crop improvement.

Testosterone (TS) is an important androgen drug and a precursor of steroid drug synthesis. Ketoreductase 2 (KR-2) (GenBank accession no. ABP64403.1) is observed to stereo-selectively catalyze the bioreduction of 4-androstene-3,17-dione (4-AD) to testosterone and contribute to the regeneration of NADH using isopropanol as a co-substrate. The Km value of KR-2 was 2.22 mmol/L with 4-AD, and the optimal pH was 6.5–7.0. The enzyme is stable and demonstrates relatively high-level enzyme activity at 40 °C. Acetone significantly inhibits this activity. This inhibition was overcome using an intermittent vacuum during the reaction process. Finally, the amount of TS reached 65.42 g/L after a 52 h reaction with 65.8 g/L 4-AD, 10% isopropanol, and 2 g/L β–NAD+ at 40 °C, with a conversion rate of 98.73%. A total of 6.15 g of TS was obtained from 6.58 g of 4-AD after the reaction and purification; the HPLC purity was 99.82%, and the overall yield was 92.81%. This enzyme provides a promising route for the green biosynthesis of testosterone for industrial applications.

Electroplated hard chromium (EPHC) has been widely used in industry due to its excellent mechanical properties, but the development of this technology is limited by environmental risks. The physical vapor deposition (PVD) process has shown promise as an alternative to EPHC for producing chromium-based coatings. In this research, we investigate the microstructure and wear resistance of pure chromium coatings using two PVD techniques, namely, magnetron sputtering ion plating (MSIP) and micro-arc ion plating (MAIP), which are compared to EPHC. To assess wear resistance, we evaluated factors such as hardness, coating base bonding force, wear rate and friction coefficient via friction and wear experiments. The results show that, in terms of microstructure, while the EPHC coating does not exhibit a strong preferred growth orientation, the PVD coatings exhibit an obvious preferred growth orientation along the (110) direction. The average grain size of the EPHC coating is the smallest, and the PVD chromium coatings show a higher hardness than the EPHC coating. The results of pin-on-disk tests show that there is little difference in friction coefficients between EPHC and MAIP chromium plating; however, the MAIP chromium coating showed an excellent specific wear rate (as low as 1.477 × 10−13 m3/Nm). The wear condition of the MAIP chromium coating is more stable than that of the EPHC coating, indicating its potential as a replacement for EPHC.

Similar to many energy projects, the evaluation of investments in natural gas projects is influenced by technical and economic uncertainties. These uncertainties include natural resource characteristics, production, decline laws, prices, taxes, benchmark yield, and so on. In China, conventional natural gas is still the dominant energy source. The investors are mainly large state-owned energy companies. Therefore, it is necessary to include the technical and economic uncertainties, as well as the investment decision and optimization problems of the enterprises in a unified analytical framework. To this end, this paper innovatively constructs such a framework. Using numerical simulations of approaches, the process of investment decision optimization by companies based on technology assessment and price forecasting is visualized in detail. The results suggest that the investment decision of the enterprise needs to consider technical and economic uncertainties in an integrated manner. It also needs to combine the business strategy and social responsibility of the enterprise in order to construct the objective function. With the availability of data, the framework and its algorithms can be used for practical evaluation of investment plans and decision supports for conventional natural gas projects. The framework can also integrate the analytical perspective of the macroeconomic and political environment to bring in a more comprehensive range of uncertainties.

Cadmium (Cd) contamination in edible agricultural products, especially in crops, has raised worldwide concerns regarding food safety consumption. This review summarizes the current knowledge of the applicable methods and perspectives for reducing Cd contamination of agricultural products. Agricultural approaches of soil amendments, irrigation management, microbial agent, and cropping patterns were systematically concluded to illustrate the developments and achievements in crop contamination management. The use of traditional soil amendments as well as novel nano-materials has contributed to producing safe crops in agricultural soil contaminated with Cd. This review provides an inspiring and promising tool for maintaining food safety by reducing Cd accumulation in edible agricultural products.

Background: This study aimed to evaluate tumor heterogeneity of metastatic breast cancer (MBC) and investigate its impact on the efficacy of pyrotinib in patients with HER2-positive MBC. Methods: MBC patients who underwent 18F-FDG PET/CT before pyrotinib treatment were included. Temporal and spatial tumor heterogeneity was evaluated by the discordance between primary and metastatic immunohistochemistry (IHC) results and baseline 18F-FDG uptake heterogeneity (intertumoral and intratumoral heterogeneity indexes: HI-inter and HI-intra), respectively. Progression-free survival (PFS) was estimated by the Kaplan–Meier method and compared by a log-rank test. Results: A total of 572 patients were screened and 51 patients were included. In 36 patients with matched IHC results, 25% of them had HER2 status conversion. Patients with homogenous HER2 positivity had the longest PFS, followed by patients with gained HER2 positivity, while patients with HER2 negative conversion could not benefit from pyrotinib (16.8 vs. 13.7 vs. 3.6 months, p < 0.0001). In terms of spatial heterogeneity, patients with high HI-intra and HI-inter had significantly worse PFS compared to those with low heterogeneity (10.6 vs. 25.3 months, p = 0.023; 11.2 vs. 25.3 months, p = 0.040). Conclusions: Temporal heterogeneity of HER2 status and spatial heterogeneity of 18F-FDG uptake could predict the treatment outcome of pyrotinib in patients with HER2-positive MBC, which provide practically applicable methods to assess tumor heterogeneity and guidance for treatment decisions.

Background: The heterogeneity of estrogen receptor (ER) expression has long been a challenge for the diagnosis and treatment strategy of metastatic breast cancer (MBC). A novel convenient method of ER detection using 18F-fluoroestradiol positron emission tomography/computed tomography (18F-FES PET/CT) offers a chance to screen and analyze MBC patients with ER uncertainty. Methods: MBC patients who received 18F-FES PET/CT were screened and patients with both FES positive (FES+) and negative (FES-) lesions were enrolled in this study. Progression-free survival (PFS) was estimated using the Kaplan–Meier method and was compared using the log-rank test. Results: A total of 635 patients were screened and 75 of 635 (11.8%) patients showed ER uncertainty; 51 patients received further treatment and were enrolled in this study. Among them, 20 (39.2%) patients received chemotherapy (CT), 21 (41.2%) patients received endocrine-based therapy (ET), and 10 (19.6%) patients received combined therapy (CT + ET). CT showed a better progression-free survival (PFS) compared with ET (mPFS 7.1 vs. 4.6 months, HR 0.44, 95% CI 0.20–0.93, p = 0.03). CT + ET did not improve PFS compared with either CT or ET alone (mPFS 4.4 months, p > 0.2). All three treatment options were well tolerated. Conclusions: 18F-FES PET/CT could identify patients with ER heterogeneity. Patients with bone metastasis are more likely to have ER heterogeneity. Patients with ER heterogeneity showed better sensitivity to CT rather than ET. Combined therapy of CT + ET did not improve the treatment outcome.

Environmental meteorological parameters (temperature, humidity, air pressure, etc.) have an important impact on short gap streamer discharge. Based on the streamer theory and particle continuity equation of gas discharge, a short gap streamer discharge simulation model is established, and the influence of environmental parameters on the dynamic development process of streamer discharge is simulated and analyzed. The short gap air gap discharge characteristics were tested in a self-made gas discharge chamber. The results show that at low humidity (relative humidity 80%), with the increase of humidity, the electron density of the streamer channel decreases, the field strength of the streamer head decreases, the development speed of the streamer slows down, and the gap breakdown voltage increase. With a decrease of gas pressure, the electron density of the streamer channel decreases, the field strength of the streamer head decreases, the development speed of the streamer increases, and the gap breakdown voltage decreases; The temperature change has little effect on the streamer development process.

Real-time quantitative PCR (RT-qPCR) is a reliable and high-throughput technique for gene expression studies, but its accuracy depends on the expression stability of reference genes. Schima superba is a fast-growing timber species with strong resistance. However, thus far, reliable reference gene identifications have not been reported in S. superba. In this study, 19 candidate reference genes were selected and evaluated for their expression stability in different tissues of S. superba. Three software programs (geNorm, NormFinder, and BestKeeper) were used to evaluate the reference gene transcript stabilities, and comprehensive stability ranking was generated by the geometric mean method. Our results show that SsuACT was the most stable reference gene and that SsuACT + SsuRIB was the best reference gene combination for different tissues. Finally, the stable and less stable reference genes were verified using SsuSND1 expression in different tissues. To our knowledge, this is the first report to verify appropriate reference genes for normalizing gene expression in S. superba for different tissues, which will facilitate the future elucidation of gene regulations in this species and useful references for relative species.

Interturn short circuits are a common fault of permanent magnet synchronous motors (PMSMs). This paper proposes a new method to detect the interturn short-circuit fault (ISCF) of a five-phase PMSM. The method first takes the command voltage and measured current of each phase winding as the original signal and then obtains the delay signal orthogonal to the original signal via Hilbert transform. Then, the generalized instantaneous reactive power of each phase can be calculated from the orthogonal voltage and current signals of each phase. Finally, the influence of the ISCF on the generalized instantaneous reactive power of each phase is analyzed under different working conditions. By comparing the difference in the generalized instantaneous reactive power of each phase, it can be determined which phase winding has the ISCF. The proposed method is verified by simulated and experimental results.

Snow accumulates on the surface of insulator string, causing a decrease in its electrical performance, seriously threatening the reliable operation of the power grid. Most previous studies have focused on iced insulators; however, there is a lack of research on snow-covered insulators. In this paper, to reveal the influencing mechanism that snow has on the electrical characteristics of insulator string, based on an artificial snowing test in a chamber, the effects of equivalent salt deposit density, applied voltage type, and snow thickness on the flashover performance of snow-covered insulators are analyzed, and the flashover process is investigated. The results show that the relationship between the arc flashover gradient and the equivalent salt deposit density is a power function with a negative exponent, which is similar to that of polluted and ice-covered insulator strings. For the insulator strings with the same snow accretion, the direct current (DC) arc flashover gradient is lower than the alternating current (AC) arc flashover gradient. The relationship between arc flashover gradient and snow thickness is also a power function. The formation of a dry band during the flashover of snow-covered insulator string is similar to the flashover of the polluted insulator, and the arc propagation along the surface of the snow-covered insulator is similar to the flashover of the iced insulator.

Icing can adversely influence electric power system security. Two main issues are caused by icing: the overload of transmission lines, and the reduction in the insulation ability of the insulators. Most previous research has focused on the flashover characteristics of ice-covered insulators, but research on the icing process of the insulator is seriously lacking. Considering the effect of icing shape, the outer airflow field of an insulator was calculated and the local collision efficiencies of water droplets (β1) were investigated according to the Lagrange algorithm. The simulation showed that the values of β1 on the insulator edge and rod are much higher than on the insulator surface, and both were significantly influenced by the wind speed and median volume diameter (MVD) of the water droplets. Based on thermal balance equations, a dynamic dry-growth icing model was established. Using the natural icing conditions of Xuefeng Mountain (China) as an example, validation experiments were conducted on a composite insulator and the climate parameters measured by multi-cylinders were used to model the icing shape and mass. The results indicate that high wind speed and low temperature increase icing rate; the icing was mainly concentrated on the windward side and the greatest horizontal thickness was generally on the insulator edge. The dry-growth model had an average error lower than 25% for icing thickness and an average error lower than 20% for icing mass, which were affected by icing roughness.

Natural polluted insulator surfaces are always coated with various kinds of soluble constituents, and those constituents affect flashover performance differentially. Currently, this fact is not considered either in laboratory experiments or field pollution degree measurements, causing the existing insulation selection method to be deficient. In this paper, a systematic study on insulator flashover voltage gradient correction involving different types of soluble pollution constituents is presented. Using a typical type glass insulator as the sample, its flashover tests, polluted by typical soluble chemicals (NaCl, NaNO3, KNO3, NH4NO3, MgSO4, Ca(NO3)2 and CaSO4), were carried out. Then, the flashover gradient correction was made by combining the flashover performance of each soluble constituent, the equivalent salt deposit density (ESDD) contribution of the seven constituents, and the saturation performance of CaSO4. The correction agreed with the flashover test results of insulator polluted by three types of soluble mixture. Research results indicate that the flashover gradient correction method proposed in this paper performs well in reducing the calculating error. It is recommended to carry out component measurements and flashover gradient correction to better select outdoor insulation configuration.