1. Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage.
- Author
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Bakshi HA, Zoubi MSA, Hakkim FL, Aljabali AAA, Rabi FA, Hafiz AA, Al-Batanyeh KM, Al-Trad B, Ansari P, Nasef MM, Charbe NB, Satija S, Mehta M, Mishra V, Gupta G, Abobaker S, Negi P, Azzouz IM, Dardouri AAK, Dureja H, Prasher P, Chellappan DK, Dua K, Webba da Silva M, El Tanani M, McCarron PA, and Tambuwala MM
- Subjects
- Animals, Antineoplastic Agents, Phytogenic, Apoptosis drug effects, Cell Cycle Proteins metabolism, Cell Line, Tumor, Cell Survival drug effects, Crocus chemistry, Cytochromes c metabolism, Female, Humans, Lipid Peroxidation drug effects, Mice, Mice, Nude, Pancreatic Neoplasms genetics, Signal Transduction drug effects, Signal Transduction genetics, Transcriptome, Xenograft Model Antitumor Assays, Carotenoids therapeutic use, Liver Diseases etiology, Liver Diseases prevention & control, Pancreatic Neoplasms drug therapy, Pancreatic Neoplasms radiotherapy, Radiation Injuries prevention & control
- Abstract
Pancreatic cancer is one of the fatal causes of global cancer-related deaths. Although surgery and chemotherapy are standard treatment options, post-treatment outcomes often end in a poor prognosis. In the present study, we investigated anti-pancreatic cancer and amelioration of radiation-induced oxidative damage by crocin. Crocin is a carotenoid isolated from the dietary herb saffron, a prospect for novel leads as an anti-cancer agent. Crocin significantly reduced cell viability of BXPC3 and Capan-2 by triggering caspase signaling via the downregulation of Bcl-2. It modulated the expression of cell cycle signaling proteins P53, P21, P27, CDK2, c-MYC, Cyt-c and P38. Concomitantly, crocin treatment-induced apoptosis by inducing the release of cytochrome c from mitochondria to cytosol. Microarray analysis of the expression signature of genes induced by crocin showed a substantial number of genes involved in cell signaling pathways and checkpoints (723) are significantly affected by crocin. In mice bearing pancreatic tumors, crocin significantly reduced tumor burden without a change in body weight. Additionally, it showed significant protection against radiation-induced hepatic oxidative damage, reduced the levels of hepatic toxicity and preserved liver morphology. These findings indicate that crocin has a potential role in the treatment, prevention and management of pancreatic cancer.
- Published
- 2020
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