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Aim Senduduk fruit (Melastoma malabathricum L) is native to Indomalaya and is believed to possess anticancer activity. This study investigated antiangiogenic and anti-inflammation effects of an ethanolic extract of Senduduk fruit (EESF) on HeLa cells. Methods Cytotoxicity was assayed in HeLa cell cultures exposed to a concentration series of 500–7.8 µg/mL of EESF. IC50 was determined with a methylthiazol-tetrazolium (MTT) cell viability assay. Antiangiogenic and anti-inflammation activity was evaluated by an immunocytochemical assay of vascular endothelial growth factor (VEGF) and tumour necrosis factor alpha (TNF-α) expression in HeLa cells cultured with 1× or 2× IC50 or as a control without EESF. Results IC50 of the EESF was 956 µg/mL. The intensity of VEGF staining indicated moderate expression in HeLa cells in response to IC50, weak expression in response to 2×IC50, and strong expression in the absence of the EESF. While the intensity of TNF-α staining indicated moderate expression in HeLa cells in response to IC50 and to the absence of EESF, and weak expression in response to 2× IC50. Conclusion Senduduk fruit extract inhibited VEGF and TNF-α expression in HeLa cells in a concentration-dependent manner.