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2. Whole genomic analysis of a porcine-like human G5P[6] rotavirus strain isolated from a child with diarrhoea and encephalopathy in Japan.

Author

Komoto S, Maeno Y, Tomita M, Matsuoka T, Ohfu M, Yodoshi T, Akeda H, and Taniguchi K

Subjects
Animals, Antigens, Viral genetics, Capsid Proteins genetics, Feces, Genotype, Humans, Japan, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Rotavirus classification, Rotavirus genetics, Rotavirus Infections virology, Sequence Analysis, DNA, Viral Nonstructural Proteins genetics, Viral Proteins genetics, Diarrhea virology, Encephalitis, Viral virology, Genome, Viral genetics, Genomics methods, Rotavirus isolation & purification, Swine virology
Abstract

An unusual rotavirus strain, Ryukyu-1120, with G5P[6] genotypes (RVA/Human-wt/JPN/Ryukyu-1120/2011/G5P[6]) was identified in a stool specimen from a hospitalized child aged 4 years who showed diarrhoea and encephalopathy. In this study, we sequenced and characterized the complete genome of strain Ryukyu-1120. On whole genomic analysis, this strain was found to have a unique genotype constellation: G5-P[6]-I5-R1-C1-M1-A8-N1-T1-E1-H1. The VP6 and NSP1 genotypes I5 and A8 are those commonly found in porcine strains. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain Ryukyu-1120 appeared to be of porcine origin. Thus, strain Ryukyu-1120 was found to have a porcine rotavirus genetic backbone and is likely to be of porcine origin. To our knowledge, this is the first report of whole-genome-based characterization of the emerging G5P[6] strains in Asian countries. Our observations will provide important insights into the origin of G5P[6] strains and the dynamic interactions between human and porcine rotavirus strains.

Published
2013
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