1. Bradyrhizobium neotropicale sp. nov., isolated from effective nodules of Centrolobium paraense.
- Author
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Zilli JE, Baraúna AC, da Silva K, De Meyer SE, Farias ENC, Kaminski PE, da Costa IB, Ardley JK, Willems A, Camacho NN, Dourado FDS, and O'Hara G
- Subjects
- Bacterial Typing Techniques, Base Composition, Bradyrhizobium genetics, Bradyrhizobium isolation & purification, Brazil, DNA, Bacterial genetics, DNA, Ribosomal Spacer genetics, Fatty Acids chemistry, Genes, Bacterial, Molecular Sequence Data, Multilocus Sequence Typing, Nitrogen Fixation, Nucleic Acid Hybridization, RNA, Ribosomal, 16S genetics, RNA, Ribosomal, 23S genetics, Sequence Analysis, DNA, Symbiosis, Bradyrhizobium classification, Fabaceae microbiology, Phylogeny, Root Nodules, Plant microbiology
- Abstract
Root nodule bacteria were isolated from Centrolobium paraense Tul. grown in soils from the Amazon region, State of Roraima (Brazil). 16S rRNA gene sequence analysis of seven strains (BR 10247(T), BR 10296, BR 10297, BR 10298, BR 10299, BR 10300 and BR 10301) placed them in the genus Bradyrhizobium with the closest neighbours being the type strains of Bradyrhizobium paxllaeri (98.8 % similarity), Bradyrhizobium icense (98.8 %), Bradyrhizobium lablabi (98.7 %), Bradyrhizobium jicamae (98.6 %), Bradyrhizobium elkanii (98.6 %), Bradyrhizobium pachyrhizi (98.6 %) and Bradyrhizobium retamae (98.3 %). This high similarity, however, was not confirmed by the intergenic transcribed spacer (ITS) 16S-23S rRNA region sequence analysis nor by multi-locus sequence analysis. Phylogenetic analyses of five housekeeping genes (dnaK, glnII, gyrB, recA and rpoB) revealed Bradyrhizobium iriomotense EK05(T) ( = LMG 24129(T)) to be the most closely related type strain (95.7 % sequence similarity or less). Chemotaxonomic data, including fatty acid profiles [major components being C16 : 0 and summed feature 8 (18 : 1ω6c/18 : 1ω7c)], DNA G+C content, slow growth rate and carbon compound utilization patterns, supported the placement of the novel strains in the genus Bradyrhizobium. Results of DNA-DNA relatedness studies and physiological data (especially carbon source utilization) differentiated the strains from the closest recognized species of the genus Bradyrhizobium. Symbiosis-related genes for nodulation (nodC) and nitrogen fixation (nifH) placed the novel species in a new branch within the genus Bradyrhizobium. Based on the current data, these seven strains represent a novel species for which the name Bradyrhizobium neotropicale sp. nov. is proposed. The type strain is BR 10247(T) ( = HAMBI 3599(T))., (© 2014 IUMS.)
- Published
- 2014
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