1. Desulfatiferula olefinivorans gen. nov., sp. nov., a long-chain n-alkene-degrading, sulfate-reducing bacterium
- Author
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Cristiana Cravo-Laureau, Agnès Hirschler-Réa, Catherine Joulian, Cindy Labat, Robert Matheron, Institut pluridisciplinaire de recherche sur l'environnement et les matériaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, Université de la Méditerranée - Aix-Marseille 2-Université de Provence - Aix-Marseille 1, and Institut Pluridisciplinaire de Recherche sur l'Environnement et les Matériaux (IPREM)
- Subjects
DNA, Bacterial ,Deltaproteobacteria ,Stereochemistry ,Molecular Sequence Data ,Alkenes ,DNA, Ribosomal ,Waste Disposal, Fluid ,Microbiology ,03 medical and health sciences ,Phylogenetics ,RNA, Ribosomal, 16S ,Sulfate-reducing bacteria ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,Desulfatiferula ,0303 health sciences ,Sulfur-Reducing Bacteria ,biology ,Phylogenetic tree ,030306 microbiology ,Sequence Analysis, DNA ,General Medicine ,biology.organism_classification ,16S ribosomal RNA ,6. Clean water ,Bacterial Typing Techniques ,Type species ,Biodegradation, Environmental ,Petroleum ,Phenotype ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Water Microbiology ,Bacteria - Abstract
A novel anaerobic, long-chain alkene-degrading, sulfate-reducing bacterium, strain LM2801T, was isolated from brackish sediment of a wastewater decantation facility of an oil refinery (Berre lagoon, France). Cells of strain LM2801T were Gram-negative, motile, slightly curved or vibrioid rods. Its optimum growth conditions were 30–36 °C, 6–10 g NaCl l−1 and pH 7.5. Strain LM2801T incompletely oxidized long-chain alkenes (from C14 to C23) and fatty acids (C14 to C24). The DNA G+C content was 45.5 mol%. Sequence analyses of the 16S rRNA and dsrAB genes indicated that the strain was a member of the family Desulfobacteraceae within the Deltaproteobacteria. This novel isolate possesses phenotypic and phylogenetic traits that do not allow its classification as a member of any previously described genus. Therefore, strain LM2801T is described as a member of a new genus, Desulfatiferula gen. nov., of which Desulfatiferula olefinivorans sp. nov. is the type species. The type strain of Desulfatiferula olefinivorans is LM2801T (=DSM 18843T =JCM 14469T).
- Published
- 2007