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Your search keyword '"Ellington, Matthew J."' showing total 10 results
Start Over Author "Ellington, Matthew J." Publisher microbiology society
10 results on '"Ellington, Matthew J."'

1. Discordance between different bioinformatic methods for identifying resistance genes from short-read genomic data, with a focus on Escherichia coli

Author

Davies, Timothy J., primary, Swann, Jeremy, additional, Sheppard, Anna E., additional, Pickford, Hayleigh, additional, Lipworth, Samuel, additional, AbuOun, Manal, additional, Ellington, Matthew J., additional, Fowler, Philip W., additional, Hopkins, Susan, additional, Hopkins, Katie L., additional, Crook, Derrick W., additional, Peto, Timothy E. A., additional, Anjum, Muna F., additional, Walker, A. Sarah, additional, and Stoesser, Nicole, additional

Published
2023
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6. Discordant bioinformatic predictions of antimicrobial resistance from whole-genome sequencing data of bacterial isolates: an inter-laboratory study

Author

Doyle, Ronan M., primary, O'Sullivan, Denise M., additional, Aller, Sean D., additional, Bruchmann, Sebastian, additional, Clark, Taane, additional, Coello Pelegrin, Andreu, additional, Cormican, Martin, additional, Diez Benavente, Ernest, additional, Ellington, Matthew J., additional, McGrath, Elaine, additional, Motro, Yair, additional, Phuong Thuy Nguyen, Thi, additional, Phelan, Jody, additional, Shaw, Liam P., additional, Stabler, Richard A., additional, van Belkum, Alex, additional, van Dorp, Lucy, additional, Woodford, Neil, additional, Moran-Gilad, Jacob, additional, Huggett, Jim F., additional, and Harris, Kathryn A., additional

Published
2020
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8. Novel staphylococcal species that form part of a Staphylococcus aureus-related complex: the non-pigmented Staphylococcus argenteus sp. nov. and the non-human primate-associated Staphylococcus schweitzeri sp. nov.

Author

Tong, Steven Y. C., primary, Schaumburg, Frieder, additional, Ellington, Matthew J., additional, Corander, Jukka, additional, Pichon, Bruno, additional, Leendertz, Fabian, additional, Bentley, Stephen D., additional, Parkhill, Julian, additional, Holt, Deborah C., additional, Peters, Georg, additional, and Giffard, Philip M., additional

Published
2015
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10. IS 1 -related large-scale deletion of chromosomal regions harbouring the oxygen-insensitive nitroreductase gene nfsB causes nitrofurantoin heteroresistance in Escherichia coli .

Author

Wan Y, Sabnis A, Mumin Z, Potterill I, Jauneikaite E, Brown CS, Ellington MJ, Edwards A, and Sriskandan S

Subjects
Mutation, Nitroreductases, Oxygen, Nitrofurantoin, Escherichia coli
Abstract

Nitrofurantoin is a broad-spectrum first-line antimicrobial used for managing uncomplicated urinary tract infection (UTI). Loss-of-function mutations in chromosomal genes nfsA, nfsB and ribE of Escherichia coli are known to reduce nitrofurantoin susceptibility. Here, we report the discovery of nitrofurantoin heteroresistance in E. coli clinical isolates and a novel genetic mechanism associated with this phenomenon. Subpopulations with lower nitrofurantoin susceptibility than major populations (hereafter, nitrofurantoin-resistant subpopulations) in two E. coli blood isolates (previously whole-genome sequenced) were identified using population analysis profiling. Each isolate was known to have a loss-of-function mutation in nfsA . From each isolate, four nitrofurantoin-resistant isolates were derived at a nitrofurantoin concentration of 32 mg l -1 , and a comparator isolate was obtained without any nitrofurantoin exposure. Genomes of derived isolates were sequenced on Illumina and Nanopore MinION systems. Genetic variation between isolates was determined based on genome assemblies and read mapping. Nitrofurantoin minimum inhibitory concentrations (MICs) of both blood isolates were 64 mg l -1 , with MICs of major nitrofurantoin-susceptible populations varying from 4 to 8 mg l -1 . Two to 99 c.f.u. per million demonstrated growth at the nitrofurantoin concentration of 32 mg l -1 , which is distinct from that of a homogeneously susceptible or resistant isolate. Derived nitrofurantoin-resistant isolates had 11-66 kb deletions in chromosomal regions harbouring nfsB , and all deletions were immediately adjacent to IS 1 -family insertion sequences. Our findings demonstrate that the IS 1 -associated large-scale genetic deletion is a hitherto unrecognized mechanism of nitrofurantoin heteroresistance and could compromise UTI management. Further, frequencies of resistant subpopulations from nitrofurantoin-heteroresistant isolates may challenge conventional nitrofurantoin susceptibility testing in clinical settings.

Published
2023
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