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Quantitative analysis on the planktonic foraminiferal assemblages in the Late Pliocene - Early Pleistocene segment of the Capo Rossello section evidences the same number of relative abundance fluctuations of the Globigerinoides ruber taxonomic unit recorded in the same stratigraphic interval of other Mediterranean sequences. Between the Globorotalia inflata FO and the Discoaster brouweri LO 4 relative abundance fluctuations are present. Since the D. brouweri LO is recorded in the Mediterranean basin below the base of the Olduvai magnetic event and in coincidence of relative abundance fluctuation 73, the Gt. inflata FO is coincident with fluctuation 80. The first appearance ol Globorotalia truncatulinoides is recorded on the second fluctuation above the Gt. inflata FO and on the second fluctuation below the D. brouwueri LO. Therefore the Gt. truncatulinoides FO is coincident with fluctuation 77. The absolute age proposed for these 3 bio-events is 1.99,2.07 and 2.13 MA respectively. The most important calcareous nannofossil events, recognized by quantitative analysis, have been correlated to the abundance fluctuations of the Globigerinoides ruber taxonomic unit.

A high-resolution geochemical and micropaleontological data set has been obtained from the uppermost Langhian/lower Serravallian marly sediments of the Ras il Pellegrin section (Malta Island). A combination of the recorded stratal organization with the results of spectral analyses performed on CaCO3 data and faunal signals shows a dominance of the classic Milankovitch periodicities as modulating forcing of the studied succession. The application of band-pass filters allowed us to select the different Milankovitch frequencies (precession, obliquity and short- and long-eccentricity) from the original faunal and geochemical signals and to compare them with the same components of the astronomical curve. The reliability of the short-term astronomical tuning has been tested by using the larger-order cyclicity (100-400 kyr) as control. The good match of the different records with the selected insolation curve is consistent with the results of the cross-spectral analysis showing high coherency values in all the considered frequency bands. The calibration provided astronomical ages for the sedimentary cycles and consequently for all the bioevents recorded in the section. In particular, an age of 13.59 Ma has been obtained for the last occurrence (LO) of Sphenolitus heteromorphus, at present considered the best bioevent useful for recognizing the Langhian/Serravallian boundary. Moreover, an age of 12.62 Ma has been obtained for the first occurrence (FO) of Paragloborotalia partimlabiata.

The relative position of 30 main bioevents pertaining to calcareous nannofossils and planktonic foraminifera was identified in the time interval between 13.75 Ma and 10.50 Ma, based on the quantitative study of the those microfossils in three Mediterranean sections spanning the late Langhian – lower Tortonian stratigraphic interval. The events were correlated to the astronomic target curve using a cyclostratigraphic approach, resulting in a very detailed biostratigraphic and biochronologic subdivision of the interval. The zonal scheme proposed by Fornaciari et al. (1996) was adopted for the calcareous nannofossils, but three subzones were identified in the MMN7 Zone. For the planktonic foraminifera reference is made to the zonal scheme recently proposed by Foresi et al. (1998), slightly modified in order to increase its biostratigraphic resolution. The age of all the zonal boundaries is reported.

Results of an integrated biostratigraphic (calcareous nannofossils and planktonic foraminifers) study of the uppermost Langhian/lower Serravallian Ras il-Pellegrin section (Fomm ir-Rih Bay – Malta) are presented. This high resolution study allowed us to recognise several useful lower Serravallian bio-events in the Mediterranean and to provide a detailed distribution pattern of the recognised taxa. The astrochronological calibration, reported in a different paper of this volume, provided absolute ages of the bio-events of the studied section. The LO (Last Occurrence) of Globorotalia peripheroronda at 13.39 Ma, the Paragloborotalia partimlabiata FO (First Occurrence) at 12.62 Ma and the Paragloborotalia mayeri FCO (First Common Occurrence) at 12.34 Ma among the planktonic foraminifera, and the Sphenolithus heteromorphus LO at 13.59 Ma, the Cyclicargolithus floridanus LCO (Last Common Occurrence) at 13.39 Ma, the Reticulofenestra pseudoumbilicus FCO at 13.32 Ma, the Calcidiscus macintyrei FO at 12.57 Ma and the Calcidiscus premacintyrei LCO at 12.51 Ma among the calcareous nannofossils, were recorded. Moreover, our results confirm the LO of S. heteromorphus as a fairly synchronous event in the Mediterranean and in the Atlantic and Pacific Oceans. Therefore, we suggest a lithological level near or coincident with this event for the definition of the GSSP of the Serravallian. The Ras il-Pellegrin section may be considered a good candidate for this definition.

The upper Serravallian-Lower Tortonian Case Pelacani section in Sicily is represented by the epipelagic sediments of the Tellaro Formation. Along the section, which was sampled in four easily correlatable segments, a more or less continuous sequence of lithologic couplets, with whitish marls and blackish levels in the lower part and whitish marls and whitish more indurated levels in the upper part are present. The distribution of the most common taxa of planktonic foraminifera and calcareous nannofossils was quantitatively estimated in 316 samples, collected every 20 cm along the section which is 66.35 m thick. This allowed to identify eleven planktonic foraminifera (but only the eight marker events were quantitatively estimated) and five calcareous nannofossil bio-events. Their astrochronology is reported by correlation with the cyclostratigraphic results obtained for this section in a different paper. The ages are well comparable with the ages published for the same events in other Mediterranean sections. Paleomagnetic analysis along 30 meters in the central part of the section gave puzzling results and therefore no reliable magnetostratigraphy could be obtained. The Case Pelacani section, straddling the stratigraphic interval between slightly below the first occurrence and slightly above the first regular occurrence of Neogloboquadrina acostaensis , is a good candidate for the definition of the GSSP (Global Stratigraphic Section and Point) of the Tortonian. Independently from the selected section, we suggest to define the Serravallian/Tortonian boundary in a level coincident or near the last occurrence of Paragloborotalia siakensis, as already proposed, or coincident with or near the last common occurrence of Globigerinoides subquadratus. This latter level virtually coincides with the first common occurrence of Globigerinoides obliquus obliquus and is slightly above the last common occurrence of Discoaster kugleri, which occurs only 2.25 meters (and 3 precessional cycles) below in the studied section.

A high resolution cyclostratigraphic study was carried out on a cyclicaly bedded succession of late Middle Miocene deep marine deposits from the Tremiti Islands, Adriatic sea (Italy). Astronomical calibration of the sedimentary cycles provides absolute ages for different calcareous plankton bioevents, widely used for intra Mediterranean correlation, in the interval between 11.12 and 12.60 Ma. The sedimentary record of the S. nicola composite section consists of an alternation of indurated, withish coloured, CaCo3 rich and grey less indurated, CaCo3 poor marly beds, at times replaced by red coloured CaCo3 poor marls. Results of direct correlation between the La 90 (1,1) solution of the insolation curve and the cyclic lithologic patterns occurring in the studied sections, combined with results of spectral methodologies applied on the climate sensitive data (CaCo3 and Globigerinoides) showed that the classic Milankovitch periodicity can be represented through the modulation forcing of the studied sedimentary records.

We performed a cyclostratigraphic study of a sedimentary sequence (Case Pelacani section) outcropping in the south-eastern margin of Sicily (Italy) and covering the Upper Serravallian/Lower Tortonian stratigraphic interval. Calcareous plankton biostratigraphic data reported in another paper proved that all the sequence of bio-events generally reported from just below and above the S/T boundary is present in the section. They allowed a detailed correlation with the Gibliscemi section. Preliminary paleomagnetic data suggest that a secondary remagnetization component prevents the recognition of the correct sequence of paleomagnetic chrons along the studied interval. The sedimentary record has been compared, on the basis of an integrated calcareous plankton biostratigraphy, with that of the Gibliscemi section. Cyclostratigraphic analysis of the lithological patterns recognized throughout the succession and the application of spectral methodologies to the abundance fluctuations of the planktonic foraminifer Globigerinoides quadrilobatus highlighted the presence in the signal of the classic Milankovitch frequencies (precession, obliquity and eccentricity). Correlation of the lithological patterns and of the different frequency bands extracted by numerical filtering from the faunal record with the same components modulating the insolation curve provided an astronomic calibration of the sedimentary record and, consequently, a precise age for all the calcareous plankton bioevents recognized throughout the studied interval.

The planktonic foraminifer and calcareous nannofossil content of two Middle Miocene sections of the Tremiti Islands (Southern Adriatic Sea) have been studied. The two sections are composed of marly limestones rich in calcareous plankton which show cyclic alternations of indurated (higher carbonate content) whitish and less indurated grey or reddish beds. The two sections represent a succession with a total thickness of 38 m. Samples have been collected at a mean spacing of 10-15 cm; qualitative analyses were performed on one sample per meter but quantitative analyses were made for each sample. The abundance fluctuations of several marker species proved to be a very useful tool to correlate the two sections. The astronomical calibration of the sedimentary cycles provided absolute ages for all the recognised calcareous plankton bioevents.

Detailed quantitative analyses of the benthic foraminiferal assemblage from the base of the Pliocene sections (M Pl 1 biozone) at Eraclea Minoa and the Capo Rossello area (southern Sicily) documented fluctuating paleoecological conditions indicative of a dysaerobic bottom water environment. In particular, we identify the Uvigerina pygmaea-peregrina Event, which in the area studied covers the upper part of the M Pl 1 biozone. According to the integrated calcareous plankton biostratigraphy and to cyclostratigraphy based on fluctuations in the relative abundance of fauna in the planktonic foraminiferal assemblage, the base of the Uvigerina pygmaea-peregrina Event coincides with cycle 6a and its top coincides with cycle 10 in the Sicilian sections.A similar temporal correlation is seen in the Tyrrhenian basin (ODP Site 652). On the contrary, the Uvigerina pygmaea-peregrina Event covers a longer time interval in the Jonian basin, where it is recognizable from cycle 4 to cycle 12. The Uvigerina pygmaea-peregrina Event is time equivalent with an interval characterized by cyclically repeated sapropels in the eastern Mediterranean basin. As a matter of fact, 10 sapropels are present in the deep-sea sediments (ODP Site 969) and 10 Uvigerina pygmaea-peregrina peaks are present in the Roccella Ionica - Capo Spartivento composite section in the M Pl 1- lowest M Pl 2 biozones. The diachronous termination of the Uvigerina pygmaea-peregrina Event may indicate that well-oxygenated bottom conditions were established earlier in the western than in the eastern Mediterranean basin. Both the sapropels interval in the eastern Mediterranean and low-oxygen bottom conditions in the Jonian basin occurred during a time interval which straddles a minimum of eccentricity in the astronomical record.