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Acne vulgaris usually associated with bacterial infections which develops antibiotic resistance in short time. To overcome the resistance, high dose of antibiotic must be directed toward the infected site for appropriate treatment period. For this reason, ciprofloxacin HCl in liposomes may have a chance to concentrate at hair follicles and sebaceous gland and treat skin infections and acne. Aim of this study was to find out the best formula in terms of entrapment efficiency and elasticity among the prepared formula in addition to find the effect of method of preparation on prepared liposomes. Multiple formulas were prepared using 100 mg of ciprofloxacin HCl, 700 mg of phospahtidylcholine with different concentrations of cholesterol (10, 20, 30 and 40 mg). Formulas contain 20 and 30 mg of cholesterol were selected to study the effect of sodium deoxycholate. Rotary evaporator and bath sonication methods were utilized to prepare liposomes. Liposomes properties as entrapment efficiency, vesicles size, relative deformability (elasticity) and pH were studied. Regarding formulas prepared by rotary evaporator, formula which contains 30 mg cholesterol and 25 mg sodium deoxycholate has higher entrapment efficiency (77.24 %). While the highest entrapment efficiency (81.99 %) was obtained from formula contain 20 mg cholesterol alone prepared by sonication method. Liposomes are relatively small in size and ranged between 8.89 - 89.9 microns. Sonication method gives liposomes with higher elasticity (relative deformability equal to 7 minutes) for both formulas prepared by 20 mg cholesterol and 50 and 75 mg sodium deoxycholate respectively. No significant effect for method of preparation on pH of the formulas were observed as the pH values ranged between 4.4 - 5.5.