1. The Effect of Molecular Weight on the Antibacterial Activity of N,N,N-Trimethyl Chitosan (TMC)
- Author
-
Dorota Cibor, Priyanka Sahariah, Dawid Stawski, Dorota Zielińska, Martha Á. Hjálmarsdóttir, Már Másson, Lyfjafræðideild (HÍ), Faculty of Pharmaceutical Sciences (UI), Heilbrigðisvísindasvið (HÍ), School of Health Sciences (UI), Háskóli Íslands, and University of Iceland
- Subjects
Acidic hydrolysis ,02 engineering and technology ,medicine.disease_cause ,01 natural sciences ,Chitosan ,lcsh:Chemistry ,chemistry.chemical_compound ,antibacterial activity ,acidic hydrolysis ,lcsh:QH301-705.5 ,Spectroscopy ,chemistry.chemical_classification ,N,N,N-trimethyl chitosan ,Molecular Structure ,Hydrolysis ,General Medicine ,Polymer ,021001 nanoscience & nanotechnology ,Anti-Bacterial Agents ,Computer Science Applications ,Staphylococcus aureus ,Molar mass distribution ,0210 nano-technology ,Antibacterial activity ,Microbial Sensitivity Tests ,010402 general chemistry ,Article ,Catalysis ,Inorganic Chemistry ,N-trimethyl chitosan ,medicine ,average molecular weight ,High activity ,Physical and Theoretical Chemistry ,Molecular Biology ,Lyfjaefnafræði ,Average molecular weight ,Organic Chemistry ,0104 chemical sciences ,Molecular Weight ,chemistry ,lcsh:Biology (General) ,lcsh:QD1-999 ,Gentamicins ,chitosan ,Nuclear chemistry - Abstract
Publisher's version (útgefin grein), N,N,N-trimethyl chitosan (TMC) with 93% degree of trimethylation was synthesized. TMC and the chitosan starting material were subjected to acidic hydrolysis to produce 49 different samples with a reduced average molecular weight (Mw) ranging from 2 to 144 kDa. This was done to allow the investigation of the relationship between antibacterial activity and Mw over a wide Mw range. NMR investigation showed that hydrolysis did not affect the degree of trimethylation (DSTRI) or the structure of the polymer backbone. The activity of TMC against Staphylococcus aureus (S. aureus) increased sharply with Mw until a certain Mw value (critical Mw for high activity, CMW) was reached. After the CMW, the activity was not affected by a further increase in the Mw. A similar pattern of activity was observed for chitosan. The CMW was determined to be 20 kDa for TMC and 50 kDa for chitosan., This study was initially funded by the Icelandic Research Fund (Grant No. 120443021) and by the EEA Scholarship and Training Fund. The final work was funded by the Icelandic Technical Development fund through the Marine Biotech ERA-NET Project—Blueteeth (Grant No. 179012-0612).
- Published
- 2019
- Full Text
- View/download PDF