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The development of new fermentative strategies exploiting the potential of different wine-related species is of great interest for new winemaking conditions and consumer preferences. One of the most promising non-conventional approaches to wine fermentation is the combined use of deacidifying and acidifying yeasts. Lachancea thermotolerans shows several other properties besides lactic acid production; among them, high malic acid consumption is of great interest in the production of red wines for avoiding undesirable refermentations once bottled. The combination of a L. thermotolerans strain that is able to consume malic acid with a Schizosaccharomyces pombe strain helps to ensure malic acid elimination during alcoholic fermentation while increasing the final acidity by lactic acid production. To properly assess the influence of this alternative strategy, we developed combined fermentations between specific strains of L. thermotolerans and S. pombe under sequential inoculation. Both species showed a great performance under the studied conditions, influencing not only the acidity but also the aromatic compound profiles of the resulting wines. The new proposed biotechnological strategy reduced the final concentrations of ethanol, malic acid and succinic acid, while it increased the concentrations of lactic acid and esters.

Hydrogen can drastically degrade the mechanical properties of a variety of metallic materials. The so-called hydrogen environment embrittlement of austenitic CrNi-type steels is usually accompanied by the formation of secondary surface cracks, which can be investigated in order to assess the embrittlement process. The occurrence of hydrogen-induced cracks is often related to element segregation effects that locally impact the austenite stability. Since there is as yet a lack of investigation methods that can visualize both structures three-dimensionally, the present study investigates the imageability of hydrogen-induced cracks and element segregation structures in austenitic CrNi-steel via micro-computed tomography (CT). In order to improve the X-ray visibility of segregation structures, modified versions of the reference steel, X2CrNi18-9, that contain W and Si are designed and investigated. The investigations demonstrated that small differences in the X-ray attenuation, caused by the W or Si modifications, can be detected via CT, although segregation structures could not be imaged due to their small size scale and image noise. Hydrogen-induced cracks were characterized successfully; however, the detection of the smaller cracks is limited by the resolution capability.

This study was performed with the aim of characterizing the fermentative performance of three commercial strains of Torulaspora delbrueckii and their impact on the production of volatile and non-volatile compounds. Laboratory-scale single culture fermentations were performed using a commercial white grape juice. The addition of commercial nutrient products enabled us to test the yeasts under two different nutrient conditions. The addition of nutrients promoted fermentation intensity from 9% to 20 % with significant differences (p < 0.05) among the strains tested. The strain diversity together with the nutrient availability influenced the production of volatile compounds.