1. A Collaborative Evaluation of LC-MS/MS Based Methods for BMAA Analysis: Soluble Bound BMAA Found to Be an Important Fraction
- Author
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Cristina Porojan, Joop Harmsen, Miquel Lürling, Leopold L. Ilag, Christophoros Christophoridis, Nadezda Zguna, Yehudit Viner-Mozzini, Hanna Mazur-Marzec, Jussi Meriluoto, Ekaterina Chernova, Jutta Fastner, Wendy Beekman-Lukassen, Audrey Combès, Anastasia Hiskia, Christine Edwards, Maria G. Antoniou, Elisabeth J. Faassen, Triantafyllos Kaloudis, Lucie Bláhová, Srdjan Lopicic, Αντωνίου, Μαρία Γ., and Aquatic Ecology (AqE)
- Subjects
Aquatic Ecology and Water Quality Management ,Daphnia magna ,Pharmaceutical Science ,Tandem mass spectrometry ,Medical and Health Sciences ,01 natural sciences ,chemistry.chemical_compound ,Cycad ,0302 clinical medicine ,Tandem Mass Spectrometry ,6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) ,Drug Discovery ,γ-diaminobutyric acid (DAB) ,lcsh:QH301-705.5 ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,l-alanine+%28BMAA%29%22">">l-alanine (BMAA) ,Cyanobacteria Toxins ,Brain ,N-(2-aminoethyl) glycine (AEG) ,β-N-methylamino ,international ,Human Resource Management ,Environmental Risk Assessment ,alpha ,Neurotoxins ,Relative standard deviation ,α ,Fraction (chemistry) ,β-N-methylamino-, ,l<%2Fspan>-alanine+%28BMAA%29%22">">l -alanine (BMAA)α,γ-diaminobutyric acid (DAB) ,cycad ,hydrophilic interaction liquid chromatography (HILIC) ,Internal standard ,Liquid chromatography-tandem mass spectrometry (LC-MS/MS) ,phytoplankton ,seafood ,Cyanobacteria ,Article ,beta-N-methylamino-l-alanine (BMAA) ,03 medical and health sciences ,alpha,gamma-diaminobutyric acid (DAB) ,Lc ms ms ,Animals ,Trichloroacetic Acid ,Health risk ,Derivatization ,Hydrophilic interaction liquid chromatography (HILIC) ,Chromatography ,WIMEK ,010401 analytical chemistry ,Ms analysis ,Amino Acids, Diamino ,Reproducibility of Results ,Chromatography liquid ,β-N-methylamino-L-alanine (BMAA) ,Aquatische Ecologie en Waterkwaliteitsbeheer ,gamma-diaminobutyric acid (DAB) ,0104 chemical sciences ,lcsh:Biology (General) ,Daphnia ,chemistry ,Seafood ,Phytoplankton ,Clinical Medicine ,030217 neurology & neurosurgery ,Chromatography, Liquid - Abstract
Exposure to beta-N-methylamino-l-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D(3)BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%-32%), implying that D(3)BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery (
- Published
- 2016
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