1. Biological responses of ceramic bone spacers produced by green processing of additively manufactured thin meshes
- Author
-
M. Antonia De los Santos-López, Elif Bilgiç, Petek Korkusuz, Feza Korkusuz, Berna Kankilic, Joaquim Minguella-Canela, Jose Antonio Calero, Universitat Politècnica de Catalunya. Departament d'Enginyeria Mecànica, Universitat Politècnica de Catalunya. TECNOFAB - Grup de Recerca en Tecnologies de Fabricació, and Universitat Politècnica de Catalunya. CREMIT - Centre de Recerca de Motors i Instal·lacions Tèrmiques
- Subjects
implants ,02 engineering and technology ,lcsh:Technology ,Bone tissue engineering ,General Materials Science ,Biomechanics ,Ceramic ,bone tissue engineering ,Cell proliferation ,lcsh:QC120-168.85 ,Enginyeria biomèdica [Àrees temàtiques de la UPC] ,021001 nanoscience & nanotechnology ,Biological responses ,Collagen fibre ,Materials biomèdics ,visual_art ,bioceramics ,visual_art.visual_art_medium ,Enginyeria biomèdica ,0210 nano-technology ,lcsh:TK1-9971 ,additive manufacturing ,Biomedical engineering ,Materials science ,Additive manufacturing ,0206 medical engineering ,Human bone ,Enginyeria biomèdica::Biomaterials [Àrees temàtiques de la UPC] ,Porous scaffolds ,Osseointegration ,Article ,Enginyeria mecànica [Àrees temàtiques de la UPC] ,In vivo ,biological responses ,Bone formation ,Implants ,lcsh:Microscopy ,lcsh:QH201-278.5 ,lcsh:T ,Mesenchymal stem cell ,porous scaffolds ,Bioceramics ,Biomecànica ,020601 biomedical engineering ,In vitro ,cell proliferation ,lcsh:TA1-2040 ,Enginyeria mecànica::Processos de fabricació mecànica [Àrees temàtiques de la UPC] ,lcsh:Descriptive and experimental mechanics ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,lcsh:Engineering (General). Civil engineering (General) ,Biomedical materials - Abstract
Bone spacers are exclusively used for replacing the tissue after trauma and/or diseases. Ceramic materials bring positive opportunities to enhance greater osteointegration and performance of implants, yet processing of porous geometries can be challenging. Additive Manufacturing (AM) opens opportunities to grade porosity levels in a part, however, its productivity may be low due to its batch processing approach. The paper studies the biological responses yielded by hydroxyapatite with &beta, TCP (tricalcium phosphate) ceramic porous bone spacers manufactured by robocasting 2-layer meshes that are rolled in green and sintered. The implants are assessed in vitro and in vivo for their compatibility. Human bone marrow mesenchymal stem cells attached, proliferated and differentiated on the bone spacers produced. Cells on the spacers presented alkaline phosphatase staining, confirming osteogenic differentiation. They also expressed bone-specific COL1A1, BGAP, BSP, and SPP1 genes. The fold change of these genes ranged between 8 to 16 folds compared to controls. When implanted into the subcutaneous tissue of rabbits, they triggered collagen fibre formation and mild fibroblastic proliferation. In conclusion, rolled AM-meshes bone spacers stimulated bone formation in vitro and were biocompatible in vivo. This technology may give the advantage to custom produce spacers at high production rates if industrially upscaled.
- Published
- 2020
- Full Text
- View/download PDF