1. Instrumental setup for high-throughput small- and wide-angle solution scattering at the X33 beamline of EMBL Hamburg
- Author
-
Weifeng Shang, Manfred Roessle, Dmitri I. Svergun, Alexey Zozulya, Florent Cipriani, Peter V. Konarev, Alexey Kikhney, Daniel Franke, Clement E. Blanchet, Robbert Klaering, Bernd Robrahn, and Christoph Hermes
- Subjects
0303 health sciences ,Materials science ,business.product_category ,business.industry ,Scattering ,Detector ,DESY ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Sample (graphics) ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Optics ,Beamline ,ddc:540 ,Biological small-angle scattering ,0210 nano-technology ,business ,Storage ring ,030304 developmental biology ,Digital camera - Abstract
A setup is presented for automated high-throughput measurements of small-angle X-ray scattering (SAXS) from macromolecular solutions on the bending-magnet beamline X33 of EMBL at the storage ring DORIS-III (DESY, Hamburg). A new multi-cell compartment allows for rapid switching between in-vacuum and in-air operation, for digital camera assisted control of cell filling and for colour sample illumination. The beamline is equipped with a Pilatus 1 M-W pixel detector for SAXS and a Pilatus 300 k-W for wide-angle scattering (WAXS), and results from the use of the Pilatus detectors for scattering studies are reported. The setup provides a broad resolution range from 100 to 0.36 nm without the necessity of changing the sample-to-detector distance. A new optimized robotic sample changer is installed, permitting rapid and reliable automated sample loading and cell cleaning with a required sample volume of 40 µl. All the devices are fully integrated into the beamline control software system, ensuring fully automated and user-friendly operation (attended, unattended and remote) with a throughput of up to 15 measurements per hour.
- Published
- 2012
- Full Text
- View/download PDF