1. Synthesis and properties of the peptide corresponding to the ACTH-like sequence of human immunoglobulin G1.
- Author
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Mitin YV, Navolotskaya EV, Vasilenko RN, Abramov VM, and Zav'Yalov VP
- Subjects
- Adenylyl Cyclases metabolism, Amino Acid Sequence, Animals, Body Temperature drug effects, Brain metabolism, Humans, Immunoglobulin G chemistry, Immunoglobulin Heavy Chains chemistry, Mice, Molecular Sequence Data, Rabbits, Receptors, Corticotropin, Synaptic Membranes metabolism, Adrenocorticotropic Hormone metabolism, Immunoglobulin G metabolism, Peptide Fragments metabolism, Receptors, Pituitary Hormone metabolism
- Abstract
The decapeptide Val-Lys-Lys-Pro-Gly--Ser-Ser-Val-Lys-Val (termed immunocorticotropin) corresponding to the sequence 11-20 of the variable part of the human immunoglobulin G1 heavy chain was synthesized. The ACTH-like peptide was found to have an ability to increase body temperature. Intracerebroventricular injection of 5-10 micrograms of the peptide to rabbits induced an elevation of body temperature by 0.7-1.3 degrees C. The ACTH-like peptide was shown to compete with [125I]-ACTH (13-24) for binding to receptors on murine brain synaptic membranes and to activate adenylate cyclase of these membranes.
- Published
- 1993
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