1. Morphological features of IFN-γ–stimulated mesenchymal stromal cells predict overall immunosuppressive capacity.
- Author
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Klinker, Matthew W., Marklein, Ross A., Lo Surdo, Jessica L., Cheng-Hong Wei, and Bauer, Steven R.
- Subjects
MESENCHYMAL stem cells ,IMMUNOSUPPRESSIVE agents ,IMMUNOPHARMACOLOGY ,T cells ,ANTILYMPHOCYTIC serum - Abstract
Human mesenchymal stromal cell (MSC) lines can vary significantly in their functional characteristics, and the effectiveness of MSC-based therapeutics may be realized by finding predictive features associated with MSC function. To identify features associated with immunosuppressive capacity in MSCs, we developed a robust in vitro assay that uses principal-component analysis to integrate multidimensional flow cytometry data into a single measurement of MSC-mediated inhibition of T-cell activation. We used this assay to correlate single-cell morphological data with overall immunosuppressive capacity in a cohort of MSC lines derived from different donors and manufacturing conditions. MSC morphology after IFN-γ stimulation significantly correlated with immunosuppressive capacity and accurately predicted the immunosuppressive capacity of MSC lines in a validation cohort. IFN-γ enhanced the immunosuppressive capacity of all MSC lines, and morphology predicted the magnitude of IFN-γ–enhanced immunosuppressive activity. Together, these data identify MSC morphology as a predictive feature of MSC immunosuppressive function. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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