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Your search keyword '"Aoyama JJ"' showing total 2 results
Start Over Author "Aoyama JJ" Publisher national academy of sciences
2 results on '"Aoyama JJ"'

1. Dual-function Spot 42 RNA encodes a 15-amino acid protein that regulates the CRP transcription factor.

Author

Aoyama JJ, Raina M, Zhong A, and Storz G

Subjects
Base Pairing, Escherichia coli genetics, Escherichia coli metabolism, Escherichia coli Proteins metabolism, Glucose metabolism, Histidine metabolism, Operon, Promoter Regions, Genetic, Protein Binding, Temperature, Amino Acids chemistry, Escherichia coli Proteins genetics, RNA, Bacterial genetics, RNA, Small Untranslated genetics, Transcription Factors metabolism
Abstract

SignificanceDual-function RNAs base pair with target messenger RNAs as small regulatory RNAs and encode small protein regulators. However, only a limited number of these dual-function regulators have been identified. In this study, we show that a well-characterized base-pairing small RNA surprisingly also encodes a 15-amino acid protein. The very small protein binds the cyclic adenosine monophosphate receptor protein transcription factor to block activation of some promoters, raising the question of how many other transcription factors are modulated by unidentified small proteins.

Published
2022
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2. Dual-function AzuCR RNA modulates carbon metabolism.

Author

Raina M, Aoyama JJ, Bhatt S, Paul BJ, Zhang A, Updegrove TB, Miranda-Ríos J, and Storz G

Subjects
Culture Media, Escherichia coli growth & development, Escherichia coli Proteins chemistry, Escherichia coli Proteins metabolism, Galactose metabolism, Glycerol metabolism, Glycerolphosphate Dehydrogenase metabolism, Membrane Proteins chemistry, Membrane Proteins metabolism, Protein Biosynthesis, RNA, Bacterial chemistry, RNA, Bacterial metabolism, RNA, Messenger metabolism, Carbon metabolism, Escherichia coli metabolism, RNA, Bacterial physiology
Abstract

SignificanceWhile most small, regulatory RNAs are thought to be "noncoding," a few have been found to also encode a small protein. Here we describe a 164-nucleotide RNA that encodes a 28-amino acid, amphipathic protein, which interacts with aerobic glycerol-3-phosphate dehydrogenase and increases dehydrogenase activity but also base pairs with two mRNAs to reduce expression. The coding and base-pairing sequences overlap, and the two regulatory functions compete.

Published
2022
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