1. Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy
- Author
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Mauzeroll, Janine, Bard, Allen J., Owhadian, Omeed, and Monks, Terrence J.
- Subjects
Chemical microscopy -- Electric properties ,Chemical microscopy -- Usage ,Liver cancer -- Diagnosis ,Science and technology - Abstract
The cytotoxicity of menadione on hepatocytes was studied by using the substrate generation/tip collection mode of scanning electrochemical microscopy by exposing the cells to menadione and detecting the menadione-S-glutathione conjugate (thiodione) that is formed during the cellular detoxication process and is exported from the cell by an ATP-dependent pump. This efflux was electrochemically detected and allowed scanning electrochemical microscopy monitoring and imaging of single cells and groups of highly confluent live cells. Based on a constant flux model, [approximately equal to] 6 x [10.sup.6] molecules of thiodione per cell per second are exported from monolayer cultures of Hep G2 cells, electrochemistry | SECM | oxidative stress | ultramicroelectrode | glulathione
- Published
- 2004