1. Global dissemination of a multidrug resistant Escherichia coli clone
- Author
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Mark A. Schembri, Gordon Dougan, Minh-Duy Phan, Jesús Rodríguez-Baño, David L. Paterson, Danilo Gomes Moriel, Elizabeth Skippington, Álvaro Pascual, Nicola K. Petty, Brian M. Forde, Scott A. Beatson, Johann D. D. Pitout, Benjamin A. Rogers, Mark R. Davies, Kate M. Peters, Timothy R. Walsh, Mathew Upton, Mitchell Stanton-Cook, Nouri L. Ben Zakour, Makrina Totsika, [Petty,NK, Ben Zakour,NL, Stanton-Cook,M, Skippington,E, Totsika,M, Forde,BM, Phan,BM, Gomes Moriel,D, Peters,KM, Davies,M, Paterson,DL, Schembri,MA, Beatson,SA] Australian Infectious Diseases Research Centre, Brisbane, Australia. [Petty,NK, Beatson,SA] School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia. [Petty,NK] The ithree institute, University of Technology Sydney, Sydney, Australia. [Davies,M, Dougan,G] Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom. [Rogers,BA, Paterson,DL] University of Queensland Centre for Clinical Research, Royal Brisbane and Women’s Hospital Campus, The University of Queensland, Brisbane, Australia. [Rodriguez-Baño,J, Pascual,A] Unidad Clínica de Enfermedades Infecciosas y Microbiología, Hospital Universitario Virgen Macarena, Seville, Spain, Departmentos de Medicina y Microbiología, Universidad de Sevilla, Seville, Spain. [Pitout,JDD] Division of Microbiology, Calgary Laboratory Services, Calgary, Canada, Department of Pathology and Laboratory Medicine, and Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada. [Upton,M] School of Biomedical and Healthcare Sciences, Plymouth University, Plymouth, United Kingdom. [Walsh,TR] School of Medicine, Cardiff University, Cardiff, United Kingdom., Universidad de Sevilla. Departamento de Medicina, and Universidad de Sevilla. Departamento de Microbiología more...
- Subjects
medicine.disease_cause ,Genome ,Chemicals and Drugs::Heterocyclic Compounds::Heterocyclic Compounds, 2-Ring::Quinolines::Quinolones::Fluoroquinolones [Medical Subject Headings] ,Bacterial evolution ,Drug Resistance, Multiple, Bacterial ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Models, Statistical::Likelihood Functions [Medical Subject Headings] ,Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome, Microbial::Genome, Bacterial [Medical Subject Headings] ,Phylogeny ,Genetics ,Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Genetics::Genetics, Population::Phylogeography [Medical Subject Headings] ,Likelihood Functions ,Multidisciplinary ,Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Amidohydrolases::beta-Lactamases [Medical Subject Headings] ,Genomics ,Biological Sciences ,Organisms::Bacteria::Gram-Negative Bacteria::Gram-Negative Facultatively Anaerobic Rods::Enterobacteriaceae::Escherichia::Escherichia coli [Medical Subject Headings] ,Phenomena and Processes::Microbiological Phenomena::Bacterial Physiological Phenomena::Drug Resistance, Bacterial::Drug Resistance, Multiple, Bacterial [Medical Subject Headings] ,Phylogeography ,Farmacorresistencia bacteriana múltiple ,Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Computational Biology [Medical Subject Headings] ,Fluoroquinolonas ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA [Medical Subject Headings] ,Fluoroquinolones ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Models, Theoretical::Models, Biological::Models, Genetic [Medical Subject Headings] ,Sequence analysis ,Molecular Sequence Data ,Virulence ,Biology ,Polymorphism, Single Nucleotide ,beta-Lactamases ,Antibiotic resistance ,Species Specificity ,medicine ,Phenomena and Processes::Biological Phenomena::Species Specificity [Medical Subject Headings] ,Escherichia coli ,Secuencia de bases ,Phenomena and Processes::Genetic Phenomena::Genetic Variation::Polymorphism, Genetic::Polymorphism, Single Nucleotide [Medical Subject Headings] ,Molecular epidemiology ,Base Sequence ,Models, Genetic ,Computational Biology ,Sequence Analysis, DNA ,Genomic epidemiology ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Sequence Alignment [Medical Subject Headings] ,Multiple drug resistance ,Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Base Sequence [Medical Subject Headings] ,Information Science::Information Science::Information Services::Documentation::Molecular Sequence Data [Medical Subject Headings] ,Phenomena and Processes::Genetic Phenomena::Phylogeny [Medical Subject Headings] ,Mobile genetic elements ,Sequence Alignment ,Genome, Bacterial - Abstract
Escherichia coli sequence type 131 (ST131) is a globally disseminated, multidrug resistant (MDR) clone responsible for a high proportion of urinary tract and bloodstream infections. The rapid emergence and successful spread of E. coli ST131 is strongly associated with several factors, including resistance to fluoroquinolones, high virulence gene content, the possession of the type 1 fimbriae FimH30 allele, and the production of the CTX-M-15 extended spectrum β-lactamase (ESBL). Here, we used genome sequencing to examine the molecular epidemiology of a collection of E. coli ST131 strains isolated from six distinct geographical locations across the world spanning 2000-2011. The global phylogeny of E. coli ST131, determined from whole-genome sequence data, revealed a single lineage of E. coli ST131 distinct from other extraintestinal E. coli strains within the B2 phylogroup. Three closely related E. coli ST131 sublineages were identified, with little association to geographic origin. The majority of single-nucleotide variants associated with each of the sublineages were due to recombination in regions adjacent to mobile genetic elements (MGEs). The most prevalent sublineage of ST131 strains was characterized by fluoroquinolone resistance, and a distinct virulence factor and MGE profile. Four different variants of the CTX-M ESBL-resistance gene were identified in our ST131 strains, with acquisition of CTX-M-15 representing a defining feature of a discrete but geographically dispersed ST131 sublineage. This study confirms the global dispersal of a single E. coli ST131 clone and demonstrates the role of MGEs and recombination in the evolution of this important MDR pathogen. Australian National Health and Medical Research Council (NHMRC); the Australian Infectious Diseases Research Centre; ARC Discovery Early Career Researcher Award; Ministerio de Economía y Competitividad, Instituto de Salud Carlos III, the Spanish Network for Research in Infectious Diseases; Fondo de Investigación Sanitaria and Junta de Andalucía. Yes more...
- Published
- 2014