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3 results on '"Alexandre Rosa Campos"'

1. Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling

Author

Zhong Chen, William Hankey, Yue Zhao, Jeff Groth, Furong Huang, Hongyan Wang, Alexandre Rosa Campos, Jiaoti Huang, Robert G. Roeder, and Qianben Wang

Subjects
Science
Abstract

RNA Polymerase II (Pol II) recycling can influence transcription efficiency. Here the authors describe an approach aimed at facilitating the identification of factors involved in Pol II recycling and identify PAF1 complex components as mediators of recycling.

Published
2021
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2. Proteomic atlas of organ vasculopathies triggered by Staphylococcus aureus sepsis

Author

Alejandro Gómez Toledo, Gregory Golden, Alexandre Rosa Campos, Hector Cuello, James Sorrentino, Nathan Lewis, Nissi Varki, Victor Nizet, Jeffrey W. Smith, and Jeffrey D. Esko

Subjects
Science
Abstract

Vascular surfaces are rapidly remodeled during systemic inflammatory responses and sepsis. Here, the authors combine in vivo biotinylation and high-resolution mass spectrometry to characterize organ-level changes of the murine vascular cell surface proteome induced by MRSA sepsis.

Published
2019
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3. Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling

Author

Hongyan Wang, Robert G. Roeder, Zhong Chen, Yue Zhao, William Hankey, Alexandre Rosa Campos, Jiaoti Huang, Qianben Wang, Jeff Groth, and Furong Huang

Subjects
Male, Transcription, Genetic, Molecular biology, Science, General Physics and Astronomy, RNA polymerase II, Biochemistry, Article, General Biochemistry, Genetics and Molecular Biology, Transcription (biology), Cell Line, Tumor, Neoplasms, Gene expression, Humans, Gene silencing, Prostate tumors, Cancer, Cellular transcription, Multidisciplinary, biology, Chemistry, Paf1 complex, Nuclear Proteins, General Chemistry, In vitro, Cell biology, biology.protein, RNA Polymerase II, HeLa Cells, Transcription Factors
Abstract

RNA Polymerase II (Pol II) transcriptional recycling is a mechanism for which the required factors and contributions to overall gene expression levels are poorly understood. We describe an in vitro methodology facilitating unbiased identification of putative RNA Pol II transcriptional recycling factors and quantitative measurement of transcriptional output from recycled transcriptional components. Proof-of-principle experiments identified PAF1 complex components among recycling factors and detected defective transcriptional output from Pol II recycling following PAF1 depletion. Dynamic ChIP-seq confirmed PAF1 silencing triggered defective Pol II recycling in human cells. Prostate tumors exhibited enhanced transcriptional recycling, which was attenuated by antibody-based PAF1 depletion. These findings identify Pol II recycling as a potential target in cancer and demonstrate the applicability of in vitro and cellular transcription assays to characterize Pol II recycling in other disease states., RNA Polymerase II (Pol II) recycling can influence transcription efficiency. Here the authors describe an approach aimed at facilitating the identification of factors involved in Pol II recycling and identify PAF1 complex components as mediators of recycling.

Published
2021

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