1. ComplexEye: a multi-lens array microscope for high-throughput embedded immune cell migration analysis
- Author
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Zülal Cibir, Jacqueline Hassel, Justin Sonneck, Lennart Kowitz, Alexander Beer, Andreas Kraus, Gabriel Hallekamp, Martin Rosenkranz, Pascal Raffelberg, Sven Olfen, Kamil Smilowski, Roman Burkard, Iris Helfrich, Ali Ata Tuz, Vikramjeet Singh, Susmita Ghosh, Albert Sickmann, Anne-Kathrin Klebl, Jan Eike Eickhoff, Bert Klebl, Karsten Seidl, Jianxu Chen, Anton Grabmaier, Reinhard Viga, and Matthias Gunzer
- Subjects
Science - Abstract
Abstract Autonomous migration is essential for the function of immune cells such as neutrophils and plays an important role in numerous diseases. The ability to routinely measure or target it would offer a wealth of clinical applications. Video microscopy of live cells is ideal for migration analysis, but cannot be performed at sufficiently high-throughput (HT). Here we introduce ComplexEye, an array microscope with 16 independent aberration-corrected glass lenses spaced at the pitch of a 96-well plate to produce high-resolution movies of migrating cells. With the system, we enable HT migration analysis of immune cells in 96- and 384-well plates with very energy-efficient performance. We demonstrate that the system can measure multiple clinical samples simultaneously. Furthermore, we screen 1000 compounds and identify 17 modifiers of migration in human neutrophils in just 4 days, a task that requires 60-times longer with a conventional video microscope. ComplexEye thus opens the field of phenotypic HT migration screens and enables routine migration analysis for the clinical setting.
- Published
- 2023
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