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1. Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes

Author

Jeremy Vicencio, Carlos Sánchez-Bolaños, Ismael Moreno-Sánchez, David Brena, Charles E. Vejnar, Dmytro Kukhtar, Miguel Ruiz-López, Mariona Cots-Ponjoan, Alejandro Rubio, Natalia Rodrigo Melero, Jesús Crespo-Cuadrado, Carlo Carolis, Antonio J. Pérez-Pulido, Antonio J. Giráldez, Benjamin P. Kleinstiver, Julián Cerón, and Miguel A. Moreno-Mateos

Subjects
Science
Abstract

PAM requirement is a constraint for genome editing but this has been circumvented by engineered Cas9 nucleases as SpG and SpRY recognizing minimal PAM sequences. Here, the authors validate and optimize SpG and SpRY in vivo expanding the targeting landscape in animals.

Published
2022
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