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3 results on '"Laura Oudjedi"'

1. Highly efficient multicolor multifocus microscopy by optimal design of diffraction binary gratings

Author

Bassam Hajj, Laura Oudjedi, Jean-Bernard Fiche, Maxime Dahan, and Marcelo Nollmann

Subjects
Medicine, Science
Abstract

Abstract Multifocus microscopy (MFM) allows sensitive and fast three-dimensional imaging. It relies on the efficient design of diffraction phase gratings yielding homogeneous intensities in desired diffraction orders. Such performances are however guaranteed only for a specific wavelength. Here, we discuss a novel approach for designing binary phase gratings with dual color properties and improved diffraction efficiency for MFM. We simulate binary diffraction gratings with tunable phase shifts to explore its best diffraction performances. We report the design and fabrication of a binary array generator of 3 × 3 equal-intensity diffraction orders with 74% efficiency, 95% uniformity and dual color capability. The multicolor properties of this new design are highlighted by two-color MFM imaging. Finally, we discuss the basics of extending this approach to a variety of diffraction pattern designs.

Published
2017
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2. Bacterial partition complexes segregate within the volume of the nucleoid

Author

Antoine Le Gall, Diego I. Cattoni, Baptiste Guilhas, Céline Mathieu-Demazière, Laura Oudjedi, Jean-Bernard Fiche, Jérôme Rech, Sara Abrahamsson, Heath Murray, Jean-Yves Bouet, and Marcelo Nollmann

Subjects
Science
Abstract

In most bacteria and archaea, a broadly conserved mitotic-like apparatus assures the inheritance of duplicated genetic material before cell division. Here, the authors use super-resolution microscopies to dissect the activities required for proper DNA segregation through the nucleoid interior.

Published
2016
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3. Bacterial partition complexes segregate within the volume of the nucleoid

Author

Sara Abrahamsson, Baptiste Guilhas, Laura Oudjedi, Antoine Le Gall, Jean-Yves Bouet, Marcelo Nollmann, Diego I. Cattoni, Heath Murray, Jean-Bernard Fiche, Céline Mathieu-Demazière, and Jérôme Rech

Subjects
0301 basic medicine, DNA, Bacterial, animal structures, Cell division, Science, General Physics and Astronomy, Gene Expression, DNA Primase, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, Chromosome Segregation, Escherichia coli, Nucleoid, Genetics, Multidisciplinary, biology, Circular bacterial chromosome, Escherichia coli Proteins, Volume (computing), Inheritance (genetic algorithm), General Chemistry, Chromosomes, Bacterial, biology.organism_classification, Partition (database), Cell Compartmentation, 030104 developmental biology, chemistry, Biophysics, DNA, Archaea, Bacillus subtilis, Protein Binding
Abstract

Precise and rapid DNA segregation is required for proper inheritance of genetic material. In most bacteria and archaea, this process is assured by a broadly conserved mitotic-like apparatus in which a NTPase (ParA) displaces the partition complex. Competing observations and models imply starkly different 3D localization patterns of the components of the partition machinery during segregation. Here we use super-resolution microscopies to localize in 3D each component of the segregation apparatus with respect to the bacterial chromosome. We show that Par proteins locate within the nucleoid volume and reveal that proper volumetric localization and segregation of partition complexes requires ATPase and DNA-binding activities of ParA. Finally, we find that the localization patterns of the different components of the partition system highly correlate with dense chromosomal regions. We propose a new mechanism in which the nucleoid provides a scaffold to guide the proper segregation of partition complexes., In most bacteria and archaea, a broadly conserved mitotic-like apparatus assures the inheritance of duplicated genetic material before cell division. Here, the authors use super-resolution microscopies to dissect the activities required for proper DNA segregation through the nucleoid interior.

Published
2016

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