1. A secreted metal-binding protein protects necrotrophic phytopathogens from reactive oxygen species.
- Author
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Liu L, Gueguen-Chaignon V, Gonçalves IR, Rascle C, Rigault M, Dellagi A, Loisel E, Poussereau N, Rodrigue A, Terradot L, and Condemine G
- Subjects
- Anti-Infective Agents, Local pharmacology, Arabidopsis genetics, Arabidopsis Proteins genetics, Botrytis genetics, Botrytis metabolism, Carrier Proteins metabolism, Defensins genetics, Dickeya, Dimerization, Gammaproteobacteria drug effects, Gammaproteobacteria genetics, Gammaproteobacteria metabolism, Hydrogen Peroxide pharmacology, Iron-Binding Proteins genetics, Plant Diseases genetics, Siderophores genetics, Siderophores metabolism, Arabidopsis metabolism, Copper metabolism, Iron metabolism, Iron-Binding Proteins metabolism, Plant Diseases microbiology, Reactive Oxygen Species metabolism
- Abstract
Few secreted proteins involved in plant infection common to necrotrophic bacteria, fungi and oomycetes have been identified except for plant cell wall-degrading enzymes. Here we study a family of iron-binding proteins that is present in Gram-negative and Gram-positive bacteria, fungi, oomycetes and some animals. Homolog proteins in the phytopathogenic bacterium Dickeya dadantii (IbpS) and the fungal necrotroph Botrytis cinerea (BcIbp) are involved in plant infection. IbpS is secreted, can bind iron and copper, and protects the bacteria against H
2 O2 -induced death. Its 1.7 Å crystal structure reveals a classical Venus Fly trap fold that forms dimers in solution and in the crystal. We propose that secreted Ibp proteins binds exogenous metals and thus limit intracellular metal accumulation and ROS formation in the microorganisms.- Published
- 2019
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