12 results on '"Mj, Bailey"'
Search Results
2. Genomic network analysis of environmental and livestock F-type plasmid populations.
- Author
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Matlock W, Chau KK, AbuOun M, Stubberfield E, Barker L, Kavanagh J, Pickford H, Gilson D, Smith RP, Gweon HS, Hoosdally SJ, Swann J, Sebra R, Bailey MJ, Peto TEA, Crook DW, Anjum MF, Read DS, Walker AS, Stoesser N, and Shaw LP
- Subjects
- Animals, Anti-Bacterial Agents, Genomics, Phylogeny, Plasmids genetics, Livestock, beta-Lactamases genetics
- Abstract
F-type plasmids are diverse and of great clinical significance, often carrying genes conferring antimicrobial resistance (AMR) such as extended-spectrum β-lactamases, particularly in Enterobacterales. Organising this plasmid diversity is challenging, and current knowledge is largely based on plasmids from clinical settings. Here, we present a network community analysis of a large survey of F-type plasmids from environmental (influent, effluent and upstream/downstream waterways surrounding wastewater treatment works) and livestock settings. We use a tractable and scalable methodology to examine the relationship between plasmid metadata and network communities. This reveals how niche (sampling compartment and host genera) partition and shape plasmid diversity. We also perform pangenome-style analyses on network communities. We show that such communities define unique combinations of core genes, with limited overlap. Building plasmid phylogenies based on alignments of these core genes, we demonstrate that plasmid accessory function is closely linked to core gene content. Taken together, our results suggest that stable F-type plasmid backbone structures can persist in environmental settings while allowing dramatic variation in accessory gene content that may be linked to niche adaptation. The association of F-type plasmids with AMR may reflect their suitability for rapid niche adaptation., (© 2021. The Author(s).)
- Published
- 2021
- Full Text
- View/download PDF
3. Loss of a pyoverdine secondary receptor in Pseudomonas aeruginosa results in a fitter strain suitable for population invasion.
- Author
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González J, Salvador M, Özkaya Ö, Spick M, Reid K, Costa C, Bailey MJ, Avignone Rossa C, Kümmerli R, and Jiménez JI
- Subjects
- Biofilms, Siderophores, Oligopeptides, Pseudomonas aeruginosa genetics
- Abstract
The rapid emergence of antibiotic resistant bacterial pathogens constitutes a critical problem in healthcare and requires the development of novel treatments. Potential strategies include the exploitation of microbial social interactions based on public goods, which are produced at a fitness cost by cooperative microorganisms, but can be exploited by cheaters that do not produce these goods. Cheater invasion has been proposed as a 'Trojan horse' approach to infiltrate pathogen populations with strains deploying built-in weaknesses (e.g., sensitiveness to antibiotics). However, previous attempts have been often unsuccessful because population invasion by cheaters was prevented by various mechanisms including the presence of spatial structure (e.g., growth in biofilms), which limits the diffusion and exploitation of public goods. Here we followed an alternative approach and examined whether the manipulation of public good uptake and not its production could result in potential 'Trojan horses' suitable for population invasion. We focused on the siderophore pyoverdine produced by the human pathogen Pseudomonas aeruginosa MPAO1 and manipulated its uptake by deleting and/or overexpressing the pyoverdine primary (FpvA) and secondary (FpvB) receptors. We found that receptor synthesis feeds back on pyoverdine production and uptake rates, which led to strains with altered pyoverdine-associated costs and benefits. Moreover, we found that the receptor FpvB was advantageous under iron-limited conditions but revealed hidden costs in the presence of an antibiotic stressor (gentamicin). As a consequence, FpvB mutants became the fittest strain under gentamicin exposure, displacing the wildtype in liquid cultures, and in biofilms and during infections of the wax moth larvae Galleria mellonella, which both represent structured environments. Our findings reveal that an evolutionary trade-off associated with the costs and benefits of a versatile pyoverdine uptake strategy can be harnessed for devising a Trojan-horse candidate for medical interventions.
- Published
- 2021
- Full Text
- View/download PDF
4. Human antibodies targeting Zika virus NS1 provide protection against disease in a mouse model.
- Author
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Bailey MJ, Duehr J, Dulin H, Broecker F, Brown JA, Arumemi FO, Bermúdez González MC, Leyva-Grado VH, Evans MJ, Simon V, Lim JK, Krammer F, Hai R, Palese P, and Tan GS
- Subjects
- Animals, Antibodies, Monoclonal metabolism, Antibodies, Monoclonal pharmacology, Antibodies, Viral pharmacology, Chlorocebus aethiops, Disease Models, Animal, HEK293 Cells, Humans, Jurkat Cells, Mice, Mice, Knockout, Neutralization Tests, Recombinant Proteins metabolism, Recombinant Proteins pharmacology, STAT2 Transcription Factor genetics, Vero Cells, Viral Nonstructural Proteins metabolism, Zika Virus metabolism, Antibodies, Viral metabolism, Receptors, IgG metabolism, Viral Nonstructural Proteins immunology, Viral Vaccines immunology, Zika Virus immunology, Zika Virus Infection prevention & control
- Abstract
Zika virus is a mosquito-borne flavivirus closely related to dengue virus that can cause severe disease in humans, including microcephaly in newborns and Guillain-Barré syndrome in adults. Specific treatments and vaccines for Zika virus are not currently available. Here, we isolate and characterize four monoclonal antibodies (mAbs) from an infected patient that target the non-structural protein NS1. We show that while these antibodies are non-neutralizing, NS1-specific mAbs can engage FcγR without inducing antibody dependent enhancement (ADE) of infection in vitro. Moreover, we demonstrate that mAb AA12 has protective efficacy against lethal challenges of African and Asian lineage strains of Zika virus in Stat2
-/- mice. Protection is Fc-dependent, as a mutated antibody unable to activate known Fc effector functions or complement is not protective in vivo. This study highlights the importance of the ZIKV NS1 protein as a potential vaccine antigen.- Published
- 2018
- Full Text
- View/download PDF
5. ISMEJ turns 10: tracking rapid progress in microbial ecology.
- Author
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Kowalchuk GA and Bailey MJ
- Published
- 2017
- Full Text
- View/download PDF
6. Assessment of the bimodality in the distribution of bacterial genome sizes.
- Author
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Gweon HS, Bailey MJ, and Read DS
- Subjects
- Base Sequence, Biological Evolution, Ecosystem, Gene Expression Regulation, Bacterial physiology, RNA, Ribosomal, 16S genetics, Bacteria genetics, Genome Size, Genome, Bacterial, RNA, Bacterial genetics
- Abstract
Bacterial genome sizes have previously been shown to exhibit a bimodal distribution. This phenomenon has prompted discussion regarding the evolutionary forces driving genome size in bacteria and its ecological significance. We investigated the level of inherent redundancy in the public database and the effect it has on the shape of the apparent bimodal distribution. Our study reveals that there is a significant bias in the genome sequencing efforts towards a certain group of species, and that correcting the bias using species nomenclature and clustering of the 16S rRNA gene, results in a unimodal rather than the previously published bimodal distribution. The true genome size distribution and its wider ecological implications will soon emerge as we are currently witnessing rapid growth in the number of sequenced genomes from diverse environmental niches across a range of habitats at an unprecedented rate.
- Published
- 2017
- Full Text
- View/download PDF
7. Soil networks become more connected and take up more carbon as nature restoration progresses.
- Author
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Morriën E, Hannula SE, Snoek LB, Helmsing NR, Zweers H, de Hollander M, Soto RL, Bouffaud ML, Buée M, Dimmers W, Duyts H, Geisen S, Girlanda M, Griffiths RI, Jørgensen HB, Jensen J, Plassart P, Redecker D, Schmelz RM, Schmidt O, Thomson BC, Tisserant E, Uroz S, Winding A, Bailey MJ, Bonkowski M, Faber JH, Martin F, Lemanceau P, de Boer W, van Veen JA, and van der Putten WH
- Subjects
- Bacteria metabolism, Carbon chemistry, Environmental Restoration and Remediation, Fungi metabolism, Biomass, Biota physiology, Food Chain, Soil chemistry, Soil Microbiology
- Abstract
Soil organisms have an important role in aboveground community dynamics and ecosystem functioning in terrestrial ecosystems. However, most studies have considered soil biota as a black box or focussed on specific groups, whereas little is known about entire soil networks. Here we show that during the course of nature restoration on abandoned arable land a compositional shift in soil biota, preceded by tightening of the belowground networks, corresponds with enhanced efficiency of carbon uptake. In mid- and long-term abandoned field soil, carbon uptake by fungi increases without an increase in fungal biomass or shift in bacterial-to-fungal ratio. The implication of our findings is that during nature restoration the efficiency of nutrient cycling and carbon uptake can increase by a shift in fungal composition and/or fungal activity. Therefore, we propose that relationships between soil food web structure and carbon cycling in soils need to be reconsidered.
- Published
- 2017
- Full Text
- View/download PDF
8. Layered memristive and memcapacitive switches for printable electronics.
- Author
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Bessonov AA, Kirikova MN, Petukhov DI, Allen M, Ryhänen T, and Bailey MJ
- Abstract
Novel computing technologies that imitate the principles of biological neural systems may offer low power consumption along with distinct cognitive and learning advantages. The development of reliable memristive devices capable of storing multiple states of information has opened up new applications such as neuromorphic circuits and adaptive systems. At the same time, the explosive growth of the printed electronics industry has expedited the search for advanced memory materials suitable for manufacturing flexible devices. Here, we demonstrate that solution-processed MoOx/MoS2 and WOx/WS2 heterostructures sandwiched between two printed silver electrodes exhibit an unprecedentedly large and tunable electrical resistance range from 10(2) to 10(8) Ω combined with low programming voltages of 0.1-0.2 V. The bipolar resistive switching, with a concurrent capacitive contribution, is governed by an ultrathin (<3 nm) oxide layer. With strong nonlinearity in switching dynamics, different mechanisms of synaptic plasticity are implemented by applying a sequence of electrical pulses.
- Published
- 2015
- Full Text
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9. Catchment-scale biogeography of riverine bacterioplankton.
- Author
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Read DS, Gweon HS, Bowes MJ, Newbold LK, Field D, Bailey MJ, and Griffiths RI
- Subjects
- Actinobacteria genetics, Actinobacteria isolation & purification, Bacteria genetics, Bacteria isolation & purification, Bacteroidetes genetics, Bacteroidetes isolation & purification, Ecosystem, Plankton genetics, Plankton isolation & purification, Bacteria classification, Plankton classification, Rivers microbiology
- Abstract
Lotic ecosystems such as rivers and streams are unique in that they represent a continuum of both space and time during the transition from headwaters to the river mouth. As microbes have very different controls over their ecology, distribution and dispersion compared with macrobiota, we wished to explore biogeographical patterns within a river catchment and uncover the major drivers structuring bacterioplankton communities. Water samples collected across the River Thames Basin, UK, covering the transition from headwater tributaries to the lower reaches of the main river channel were characterised using 16S rRNA gene pyrosequencing. This approach revealed an ecological succession in the bacterial community composition along the river continuum, moving from a community dominated by Bacteroidetes in the headwaters to Actinobacteria-dominated downstream. Location of the sampling point in the river network (measured as the cumulative water channel distance upstream) was found to be the most predictive spatial feature; inferring that ecological processes pertaining to temporal community succession are of prime importance in driving the assemblages of riverine bacterioplankton communities. A decrease in bacterial activity rates and an increase in the abundance of low nucleic acid bacteria relative to high nucleic acid bacteria were found to correspond with these downstream changes in community structure, suggesting corresponding functional changes. Our findings show that bacterial communities across the Thames basin exhibit an ecological succession along the river continuum, and that this is primarily driven by water residence time rather than the physico-chemical status of the river.
- Published
- 2015
- Full Text
- View/download PDF
10. The future of microbial metagenomics (or is ignorance bliss?).
- Author
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Gilbert JA, Meyer F, and Bailey MJ
- Subjects
- Bacteria classification, Bacteria genetics, Computational Biology, Databases, Genetic, Information Storage and Retrieval, International Cooperation, Molecular Sequence Annotation, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA methods, Metagenomics methods, Metagenomics trends
- Published
- 2011
- Full Text
- View/download PDF
11. Sequence-based analysis of pQBR103; a representative of a unique, transfer-proficient mega plasmid resident in the microbial community of sugar beet.
- Author
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Tett A, Spiers AJ, Crossman LC, Ager D, Ciric L, Dow JM, Fry JC, Harris D, Lilley A, Oliver A, Parkhill J, Quail MA, Rainey PB, Saunders NJ, Seeger K, Snyder LA, Squares R, Thomas CM, Turner SL, Zhang XX, Field D, and Bailey MJ
- Subjects
- Beta vulgaris microbiology, Molecular Sequence Data, Plant Leaves microbiology, Plant Roots microbiology, Sequence Homology, Nucleic Acid, Plasmids genetics, Pseudomonas genetics
- Abstract
The plasmid pQBR103 was found within Pseudomonas populations colonizing the leaf and root surfaces of sugar beet plants growing at Wytham, Oxfordshire, UK. At 425 kb it is the largest self-transmissible plasmid yet sequenced from the phytosphere. It is known to enhance the competitive fitness of its host, and parts of the plasmid are known to be actively transcribed in the plant environment. Analysis of the complete sequence of this plasmid predicts a coding sequence (CDS)-rich genome containing 478 CDSs and an exceptional degree of genetic novelty; 80% of predicted coding sequences cannot be ascribed a function and 60% are orphans. Of those to which function could be assigned, 40% bore greatest similarity to sequences from Pseudomonas spp, and the majority of the remainder showed similarity to other gamma-proteobacterial genera and plasmids. pQBR103 has identifiable regions presumed responsible for replication and partitioning, but despite being tra+ lacks the full complement of any previously described conjugal transfer functions. The DNA sequence provided few insights into the functional significance of plant-induced transcriptional regions, but suggests that 14% of CDSs may be expressed (11 CDSs with functional annotation and 54 without), further highlighting the ecological importance of these novel CDSs. Comparative analysis indicates that pQBR103 shares significant regions of sequence with other plasmids isolated from sugar beet plants grown at the same geographic location. These plasmid sequences indicate there is more novelty in the mobile DNA pool accessible to phytosphere pseudomonas than is currently appreciated or understood.
- Published
- 2007
- Full Text
- View/download PDF
12. The ISME journal: multidisciplinary journal of microbial ecology.
- Author
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Kowalchuk GA, Heidelberg JF, and Bailey MJ
- Subjects
- Ecosystem, Evolution, Molecular, Genetics, Microbial, Molecular Biology, Ecology, Environmental Microbiology
- Published
- 2007
- Full Text
- View/download PDF
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