Search

Mechanical forces in the cell's natural environment have a crucial impact on growth, differentiation and behaviour. Few areas of biology can be understood without taking into account how both individual cells and cell networks sense and transduce physical stresses. However, the field is currently held back by the limitations of the available methods to apply physiologically relevant stress profiles on cells, particularly with sub-cellular resolution, in controlled in vitro experiments. Here we report a new type of active cell culture material that allows highly localized, directional and reversible deformation of the cell growth substrate, with control at scales ranging from the entire surface to the subcellular, and response times on the order of seconds. These capabilities are not matched by any other method, and this versatile material has the potential to bridge the performance gap between the existing single cell micro-manipulation and 2D cell sheet mechanical stimulation techniques.

A concentration cell is composed of two equivalent half-cells made of the same material but differing in the concentration of reactants. As these concentrations equilibrate, the increase in entropy is converted into a flow of electricity with the voltage output determined by the Nernst equation and proportional to the logarithm of the concentration ratios. However, as diffusion constantly strives to erase all concentration gradients, concentration cells produce only moderate voltages (typically tens of millivolts at room temperature) over relatively short times and, consequently, such devices have not been regarded as promising for energy storage. Here, we report a concentration cell that produces significantly higher voltages (∼ 0.5 V) for over 100 h. The key to our design is that the citric acid molecules involved in the electrode reactions are tethered onto magnetic nanoparticles, and a sharp gradient (10(7)-10(11) anode/cathode concentration ratio) is maintained at one of the electrodes by a permanent magnet external to the cell. Our cell does not result in corrosion of the electrodes, produces no harmful by-products, and can be regenerated by recoating used nanoparticles with fresh citric acid. We show that a series of such centimetre-sized cells produces enough electricity to power small electronic devices (timers and calculators) for several tens of hours. Our results illustrate how redox-active molecules that are, in themselves, non-magnetic can be effectively concentrated by magnetic fields to produce electrical energy.

The recently demonstrated extremely water-repellent surfaces with contact angles close to 180° with nearly zero hysteresis approach the fundamental limit of non-wetting. The measurement of the small but non-zero energy dissipation of a droplet moving on such a surface is not feasible with the contemporary methods, although it would be needed for optimized technological applications related to dirt repellency, microfluidics and functional surfaces. Here we show that magnetically controlled freely decaying and resonant oscillations of water droplets doped with superparamagnetic nanoparticles allow quantification of the energy dissipation as a function of normal force. Two dissipative forces are identified at a precision of ~ 10 nN, one related to contact angle hysteresis near the three-phase contact line and the other to viscous dissipation near the droplet-solid interface. The method is adaptable to common optical goniometers and facilitates systematic and quantitative investigations of dynamical superhydrophobicity, defects and inhomogeneities on extremely superhydrophobic surfaces.