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Your search keyword '"Schiavone, E. M."' showing total 5 results
Start Over Author "Schiavone, E. M." Publisher nature publishing group, specialist journals
5 results on '"Schiavone, E. M."'

1. Interstitial 9q deletion is associated with CD7+ acute leukemia of myeloid and T lymphoid lineage.

Author

Ferrara F, Scognamiglio M, Di Noto R, Schiavone EM, Poggi V, Fiorillo A, Libertini R, Vicari L, Del Vecchio L, and Sebastio L

Subjects
Adolescent, Adult, Aged, Child, Female, Humans, Immunophenotyping, Male, Antigens, CD7 analysis, Chromosome Deletion, Chromosomes, Human, Pair 9, Leukemia, Myeloid, Acute genetics, T-Lymphocytes ultrastructure
Abstract

We report the clinical, hematological and immunophenotypic characteristics from four cases of acute leukemia with interstitial deletion of chromosome 9, ie del(9)(q12-q22), as a single chromosomal abnormality. Three patients had acute myeloblastic leukemia (AML) and one T origin acute lymphoblastic leukemia (ALL). According to FAB classification, blasts were classified as M1 (two patients), M2 (one patient), and L2 (one patient). In two out of three AML cases a myelodysplastic syndrome, one AREB-t and one AREB diagnosed 6 and 11 months before respectively, preceded the onset of AML. Morphological examination showed dysgranulopoiesis, dyserythropoiesis and cytoplasmic vacuoles in two AML patients, while a strong positivity to myeloperoxidases was observed in all AML cases. As concerns immunophenotypic findings, blast cells from two of three AML patients expressed CD7 and CD34, while those from the T-ALL case displayed CD33 and CD34 along with CD7. These observations suggest that del (9q) is associated with CD7+ acute leukemia of myeloid or lymphoid lineage.

Published
1996

3. Expression and ATRA-driven modulation of adhesion molecules in acute promyelocytic leukemia.

Author

Di Noto R, Schiavone EM, Ferrara F, Manzo C, Lo Pardo C, and Del Vecchio L

Subjects
Antigens, CD metabolism, Antigens, Differentiation metabolism, Cell Adhesion, Cells, Cultured, Flow Cytometry, Humans, Immunophenotyping, Leukocyte Common Antigens metabolism, Lewis X Antigen metabolism, Lymphocyte Function-Associated Antigen-1 metabolism, Macrophage-1 Antigen metabolism, Receptors, IgG metabolism, Cell Adhesion Molecules metabolism, Leukemia, Promyelocytic, Acute metabolism, Tretinoin pharmacology
Abstract

On fresh leukemic cells taken from 30 patients with acute promyelocytic leukemia (APL) the membrane expression of a series of adhesion molecules including beta 2 integrins (CD11a/LFA-1, CD11b/Mac-1), selectin ligands (CD15/Le(x), CD15s/Le(x)) and tyrosine-phosphatase isoforms (CD45RA, CD45R0) was analyzed. The expression of these molecules was also studied in nine of these patients following the APL cells' culture with and without all-trans retinoic acid (ATRA). The fresh APL promyelocytes expressed CD45RA and CD15s on their surface, while CD11a, CD11b, CD15, and CD45R0 were constantly absent. In vitro treatment with ATRA consistently increased the expression of CD15, CD11b, and CD45R0 on leukemic promyelocytes; these changes were paralleled by a decrease of CD45RA display. The expression of sialylated antigen CD15s was fully independent from CD15 suggesting a differential enzymatic regulation within this selectin ligand system. ATRA was, however, incapable of promoting the up-regulation of CD11a in APL. As a result, asynchronous phenotype (CD11a-, CD11b+, CD15+, CD15s+/-, CD45RA-, CD45R0+) was generated that is normally undetectable on maturing myeloid cells. In order to provide a further control a case of acute agranulocytosis was also investigated, in which > 75% bone marrow cells were arrested at the promyelocyte stage; these bone marrow cells showed a surface phenotype identical to non-leukemic promyelocytes (CD11a+, CD11b+, CD15+, CD45R0+, CD45RA-) with a spontaneous ability to differentiate in vivo towards the more mature stages of myeloid differentiation. We therefore suggest that in fresh and ATRA-induced APL cells distinct, regular phenotypic changes are identifiable that are probably associated with t(15;17) and not seen in normal and activated bone marrow.

Published
1994

4. Expression and ATRA-driven modulation of adhesion molecules in acute promyelocytic leukemia.

Author

Di Noto R, Schiavone EM, Ferrara F, Manzo C, Lo Pardo C, and Del Vecchio L

Subjects
Bone Marrow metabolism, Cell Adhesion Molecules genetics, Cells, Cultured, Flow Cytometry, Granulocytes metabolism, Humans, Leukemia, Promyelocytic, Acute drug therapy, Phenotype, Cell Adhesion Molecules biosynthesis, Leukemia, Promyelocytic, Acute metabolism, Tretinoin pharmacology
Abstract

On fresh leukemic cells taken from 30 patients with acute promyelocytic leukemia (APL) the membrane expression of a series of adhesion molecules including beta 2 integrins (CD11a/LFA-1, CD11b/Mac-1), selectin ligands (CD15/Le(x), CD15s/sLex) and tyrosine-phosphatase isoforms (CD45RA, CD45R0) was analyzed. The expression of these molecules was also studied in nine of these patients following the APL cells' culture with and without all-trans retinoic acid (ATRA). The fresh APL promyelocytes expressed CD45RA and CD15s on their surface, while CD11a, CD11b, CD15, and CD45R0 were constantly absent. In vitro treatment with ATRA consistently increased the expression of CD15, CD11b, and CD45R0 on leukemic promyelocytes; these changes were paralleled by a decrease of CD45RA display. The expression of sialylated antigen CD15s was fully independent from CD15 suggesting a differential enzymatic regulation within this selectin ligand system. ATRA was, however, incapable of promoting the up-regulation of CD11a in APL. As a result, asynchronous phenotype (CD11a-, CD11b+, CD15+, CD15s+/-, CD45RA-, CD45R0+) was generated that is normally undetectable on maturing myeloid cells. In order to provide a further control a case of acute agranulocytosis was also investigated, in which > 75% bone marrow cells were arrested at the promyelocyte stage; these bone marrow cells showed a surface phenotype identical to non-leukemic promyelocytes (CD11a+, CD11b+, CD15+, CD45R0+, CD45RA-) with a spontaneous ability to differentiate in vivo towards the more mature stages of myeloid differentiation. We therefore suggest that in fresh and ATRA-induced APL cells distinct, regular phenotypic changes are identifiable that are probably associated with t(15;17) and not seen in normal and activated bone marrow.

Published
1994

5. Co-ordinate expression of T-cell antigens on acute myelogenous leukemia and of myeloid antigens on T-acute lymphoblastic leukemia. Speculation on a highly balanced bilinearity.

Author

Del Vecchio L, Finizio O, Lo Pardo C, Pane N, Schiavone EM, Vacca C, and Ferrara F

Subjects
Antigens, CD7, Antigens, Differentiation, T-Lymphocyte analysis, CD2 Antigens, CD3 Complex, Gene Rearrangement, T-Lymphocyte, HLA-DR Antigens analysis, Humans, Receptors, Antigen, T-Cell analysis, Receptors, Immunologic analysis, Antigens, CD analysis, Leukemia, Myeloid, Acute immunology, Leukemia-Lymphoma, Adult T-Cell immunology, T-Lymphocytes immunology
Published
1991

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