7 results on '"Drosophila -- Physiological aspects -- Genetic aspects -- Research"'
Search Results
2. Acid sensing by the Drosophila olfactory system
- Author
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Ai, Minrong, Min, Soohong, Grosjean, Yael, Leblanc, Charlotte, Bell, Rati, Benton, Richard, and Suh, Greg S.B.
- Subjects
Rhinencephalon -- Genetic aspects -- Research -- Physiological aspects ,Drosophila -- Physiological aspects -- Genetic aspects -- Research ,Sensory receptors -- Physiological aspects -- Genetic aspects -- Research ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
The odour of acids has a distinct quality that is perceived as sharp, pungent and often irritating (1). How acidity is sensed and translated into an appropriate behavioural response is poorly understood. Here we describe a functionally segregated population of olfactory sensory neurons in the fruitfly, Drosophila melanogaster, that are highly selective for acidity. These olfactory sensory neurons express IR64a, a member of the recently identified ionotropic receptor (IR) family of putative olfactory receptors (2). In vivo calcium imaging showed that IR64a+ neurons projecting to the DC4 glomerulus in the antennal lobe are specifically activated by acids. Flies in which the function of IR64a+ neurons or the IR64a gene is disrupted had defects in acid-evoked physiological and behavioural responses, but their responses to non-acidic odorants remained unaffected. Furthermore, artificial stimulation of IR64a+ neurons elicited avoidance responses. Taken together, these results identify cellular and molecular substrates for acid detection in the Drosophila olfactory system and support a labelled-line mode of acidity coding at the periphery., Many aversive odorants activate combinations of olfactory sensory neurons (OSNs) (3,4), complicating the dissection of the circuits that translate odour recognition into behaviour. By contrast, carbon dioxide (C[O.sub.2]), an odorant [...]
- Published
- 2010
- Full Text
- View/download PDF
3. Phenotypic robustness conferred by apparently redundant transcriptional enhancers
- Author
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Frankel, Nicolas, Davis, Gregory K., Vargas, Diego, Wang, Shu, Payre, Francois, and Stern, David L.
- Subjects
Genetic variation -- Research -- Genetic aspects -- Physiological aspects ,Genetic regulation -- Research -- Genetic aspects -- Physiological aspects ,Drosophila -- Physiological aspects -- Genetic aspects -- Research ,Transcription factors -- Physiological aspects -- Research -- Genetic aspects - Abstract
Genes include cis-regulatory regions that contain transcriptional enhancers. Recent reports have shown that developmental genes often possess multiple discrete enhancer modules that drive transcription in similar spatio-temporal patterns (1-4): primary enhancers located near the basal promoter and secondary, or 'shadow', enhancers located at more remote positions. It has been proposed that the seemingly redundant activity of primary and secondary enhancers contributes to phenotypic robustness (1,5). We tested this hypothesis by generating a deficiency that removes two newly discovered enhancers of shavenbaby (svb, a transcript of the ovo locus), a gene encoding a transcription factor that directs development of Drosophila larval trichomes (6). At optimal temperatures for embryonic development, this deficiency causes minor defects in trichome patterning. In embryos that develop at both low and high extreme temperatures, however, absence of these secondary enhancers leads to extensive loss of trichomes. These temperature-dependent defects can be rescued by a transgene carrying a secondary enhancer driving transcription of the svb cDNA. Finally, removal of one copy of wingless, a gene required for normal trichome patterning (7), causes a similar loss of trichomes only in flies lacking the secondary enhancers. These results support the hypothesis that secondary enhancers contribute to phenotypic robustness in the face of environmental and genetic variability., The cis-regulatory region of the svb gene integrates inputs from multiple gene regulatory networks to generate a complex pattern of expression in the embryonic epidermis of insect species (6,8). SVB [...]
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- 2010
- Full Text
- View/download PDF
4. CBP/p300-mediated acetylation of histone H3 on lysine 56
- Author
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Das, Chandrima, Lucia, M. Scott, Hansen, Kirk C., and Tyler, Jessica K.
- Subjects
Histones -- Physiological aspects -- Genetic aspects -- Research ,Drosophila -- Physiological aspects -- Genetic aspects -- Research ,DNA binding proteins -- Physiological aspects -- Research -- Genetic aspects ,Environmental issues ,Science and technology ,Zoology and wildlife conservation ,Physiological aspects ,Research ,Genetic aspects - Abstract
Acetylation within the globular core domain of histone H3 on lysine 56 (H3K56) has recently been shown to have a critical role in packaging DNA into chromatin following DNA replication and repair in budding yeast (1,2). However, the function or occurrence of this specific histone mark has not been studied in multicellular eukaryotes, mainly because the Rtt109 enzyme that is known to mediate acetylation of H3K56 (H3K56ac) is fungal-specific (3,4). Here we demonstrate that the histone acetyl transferase CBP (also known as Nejire) in flies and CBP and p300 (Ep300) in humans acetylate H3K56, whereas Drosophila Sir2 and human SIRT1 and SIRT2 deacetylate H3K56ac. The histone chaperones ASF1A in humans and Asf1 in Drosophila are required for acetylation of H3K56 in vivo, whereas the histone chaperone CAF-1 (chromatin assembly factor 1) in humans and Caf1 in Drosophila are required for the incorporation of histones bearing this mark into chromatin. We show that, in response to DNA damage, histones bearing acetylated K56 are assembled into chromatin in Drosophila and human cells, forming foci that colocalize with sites of DNA repair. Furthermore, acetylation of H3K56 is increased in multiple types of cancer, correlating with increased levels of ASF1A in these tumours. Our identification of multiple proteins regulating the levels of H3K56 acetylation in metazoans will allow future studies of this critical and unique histone modification that couples chromatin assembly to DNA synthesis, cell proliferation and cancer., H3K56 acetylation has a critical role in regulating chromatin assembly following DNA synthesis (1,2), chromatin disassembly during transcriptional activation (5) and cell survival (1) in yeast. Although H3K56ac clearly exists [...]
- Published
- 2009
5. Fused has evolved divergent roles in vertebrate Hedgehog signalling and motile ciliogenesis
- Author
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Wilson, Christopher W., Nguyen, Catherine T., Chen, Miao-Hsueh, Yang, Jehn-Hsiahn, Gacayan, Rhodora, Huang, Jie, Chen, Jau-Nian, and Chuang, Pao-Tien
- Subjects
Cilia and ciliary motion -- Physiological aspects -- Genetic aspects -- Research ,Drosophila -- Physiological aspects -- Genetic aspects -- Research ,Cellular signal transduction -- Physiological aspects -- Genetic aspects -- Research ,Environmental issues ,Science and technology ,Zoology and wildlife conservation ,Physiological aspects ,Genetic aspects ,Research - Abstract
Hedgehog (Hh) signalling is essential for several aspects of embryogenesis (1,2). In Drosophila, Hh transduction is mediated by a cytoplasmic signalling complex (3-5) that includes the putative serine-threonine kinase Fused [...]
- Published
- 2009
6. Temporal identity in axonal target layer recognition
- Author
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Petrovic, Milan and Hummel, Thomas
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Synapses -- Physiological aspects -- Research ,Axons -- Physiological aspects -- Genetic aspects -- Research ,Drosophila -- Physiological aspects -- Genetic aspects -- Research ,Environmental issues ,Science and technology ,Zoology and wildlife conservation ,Physiological aspects ,Genetic aspects ,Research - Abstract
The segregation of axon and dendrite projections into distinct synaptic layers is a fundamental principle of nervous system organization and the structural basis for information processing in the brain (1). [...]
- Published
- 2008
7. Immunology: Cascade into clarity
- Author
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Sutterwala, Fayyaz S. and Flavell, Richard A.
- Subjects
Genetic regulation -- Research -- Physiological aspects -- Genetic aspects ,Drosophila -- Physiological aspects -- Genetic aspects -- Research ,Genetic transcription -- Research -- Genetic aspects -- Physiological aspects ,DNA binding proteins -- Physiological aspects -- Research -- Genetic aspects ,Environmental issues ,Science and technology ,Zoology and wildlife conservation ,Physiological aspects ,Research ,Genetic aspects - Abstract
Author(s): Fayyaz S. Sutterwala [1]; Richard A. Flavell [2] The availability of powerful genetic tools to study the fruitfly Drosophila melanogaster , and the striking similarities of this insect's immune [...]
- Published
- 2008
- Full Text
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