1. Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells.
- Author
-
Yamashita K, Tomokiyo A, Ono T, Ipposhi K, Alhasan MA, Tsuchiya A, Hamano S, Sugii H, Yoshida S, Itoyama T, and Maeda H
- Subjects
- Aluminum Compounds chemistry, Calcium metabolism, Calcium Compounds chemistry, Cell Line, Drug Combinations, Humans, Osteoblasts metabolism, Oxides chemistry, Periodontal Ligament metabolism, Silicates chemistry, Sodium Hypochlorite chemistry, Stem Cells metabolism, Surface Properties drug effects, Aluminum Compounds pharmacology, Calcium Compounds pharmacology, Cell Differentiation drug effects, Osteoblasts drug effects, Oxides pharmacology, Periodontal Ligament drug effects, Root Canal Filling Materials pharmacology, Silicates pharmacology, Sodium Hypochlorite pharmacology, Stem Cells drug effects
- Abstract
White mineral trioxide aggregate (WMTA) is a root canal treatment material, which is known to exhibit a dark brown color when in contact with sodium hypochlorite solution (NaOCl). This study aimed to investigate the effects of NaOCl on the surface properties of WMTA discs and WMTA-induced osteoblastic differentiation of periodontal ligament stem cells (PDLSCs). Mixed WMTA (ProRoot MTA) was filled into the molds to form WMTA discs. These discs were immersed in distilled water (D-WMTA) or 5% NaOCl (Na-WMTA). Their surface structures and Ca
2+ release level was investigated. Moreover, they were cultured with a clonal human PDLSC line (line 1-17 cells). The main crystal structures of Na-WMTA were identical to the structures of D-WMTA. Globular aggregates with polygonal and needle-like crystals were found on D-WMTA and Na-WMTA, which included Ca, Si, Al, C and O. However, many amorphous structures were also identified on Na-WMTA. These structures consisted of Na and Cl, but did not include Ca. NaOCl immersion also reduced Ca2+ release level from whole WMTA discs. Line 1-17 cells cultured with D-WMTA formed many mineralized nodules and exhibited high expression levels of osteoblast-related genes. However, cells incubated with Na-WMTA generated a small number of nodules and showed low expression levels of osteoblast-related genes. These results indicated that NaOCl reduced Ca2+ release from WMTA by generating amorphous structures and changing its elemental distribution. NaOCl may also partially abolish the ability of WMTA to stimulate osteoblastic differentiation of PDLSCs., (© 2021. The Author(s).)- Published
- 2021
- Full Text
- View/download PDF