1. Characterization and application of recombinant Bovine Leukemia Virus Env protein.
- Author
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Tomé-Poderti L, Olivero-Deibe N, Carrión F, Portela MM, Obal G, Cabrera G, Bianchi S, Lima A, Addiego A, Durán R, Moratorio G, and Pritsch O
- Subjects
- Animals, Cattle, Mice, Enzootic Bovine Leukosis virology, Cell Line, Gene Products, env genetics, Gene Products, env metabolism, Gene Products, env immunology, Leukemia Virus, Bovine genetics, Leukemia Virus, Bovine immunology, Recombinant Proteins genetics, Viral Envelope Proteins genetics, Viral Envelope Proteins immunology, Viral Envelope Proteins metabolism, Antibodies, Viral immunology
- Abstract
The Bovine Leukemia Virus (BLV) Envelope (Env) glycoprotein complex is instrumental in viral infectivity and shapes the host's immune response. This study presents the production and characterization of a soluble furin-mutated BLV Env ectodomain (sBLV-EnvFm) expressed in a stable S2 insect cell line. We purified a 63 kDa soluble protein, corresponding to the monomeric sBLV-EnvFm, which predominantly presented oligomannose and paucimannose N-glycans, with a high content of core fucose structures. Our results demonstrate that our recombinant protein can be recognized from specific antibodies in BLV infected cattle, suggesting its potential as a powerful diagnostic tool. Moreover, the robust humoral immune response it elicited in mice shows its potential contribution to the development of subunit-based vaccines against BLV., (© 2024. The Author(s).)
- Published
- 2024
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