1. The yeast DOA4 gene encodes a deubiquitinating enzyme related to a product of the human tre-2 oncogene.
- Author
-
Papa FR and Hochstrasser M
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Endosomal Sorting Complexes Required for Transport, Fungal Proteins metabolism, Humans, Mice, Mice, Nude, Molecular Sequence Data, Mutation, Oncogene Proteins, Fusion genetics, Open Reading Frames, Phenotype, Proto-Oncogene Proteins, Recombinant Fusion Proteins metabolism, Saccharomyces cerevisiae enzymology, Sequence Homology, Amino Acid, Ubiquitin Thiolesterase, Endopeptidases, Fungal Proteins genetics, Genes, Fungal, Oncogene Proteins, Oncogenes, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins, Ubiquitins metabolism
- Abstract
Modification of specific intracellular proteins by ubiquitin targets them for degradation. We describe a yeast enzyme, Doa4, that is integral to the degradation of ubiquitinated proteins and is required in diverse physiological processes. Doa4 appears to function late in the proteolytic pathway by cleaving ubiquitin from substrate remnants still bound to protease. The human tre-2 oncogene encodes a deubiquitinating enzyme similar to Doa4, indicating a role for the ubiquitin system in mammalian growth control.
- Published
- 1993
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