1. Downregulation of pectin biosynthesis gene GAUT4 leads to reduced ferulate and lignin-carbohydrate cross-linking in switchgrass.
- Author
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Li M, Yoo CG, Pu Y, Biswal AK, Tolbert AK, Mohnen D, and Ragauskas AJ
- Subjects
- Coumaric Acids chemistry, Glucuronosyltransferase metabolism, Lignin chemistry, Magnetic Resonance Spectroscopy, Polysaccharides metabolism, Carbohydrate Metabolism, Coumaric Acids metabolism, Glucuronosyltransferase genetics, Lignin metabolism, Panicum metabolism, Pectins biosynthesis
- Abstract
Knockdown (KD) expression of GAlactUronosylTransferase 4 ( GAUT4 ) in switchgrass improves sugar yield and ethanol production from the biomass. The reduced recalcitrance of GAUT4 -KD transgenic biomass is associated with reduced cell wall pectic homogalacturonan and rhamnogalacturonan II content and cross-linking, and the associated increases in accessibility of cellulose to enzymatic deconstruction. To further probe the molecular basis for the reduced recalcitrance of GAUT4 -KD biomass, potential recalcitrance-related factors including the physicochemical properties of lignin and hemicellulose are investigated. We show that the transgenic switchgrass have a lower abundance of ferulate and lignin-carbohydrate complex cross-linkages, reduced amounts of residual arabinan and xylan in lignin-enriched fractions after enzymatic hydrolysis, and greater coalescence and migration of lignin after hydrothermal pretreatment in comparison to the wild-type switchgrass control. The results reveal the roles of both decreased lignin-polymer and pectin cross-links in the reduction of recalcitrance in PvGAUT4 -KD switchgrass., Competing Interests: The authors declare no competing interests.
- Published
- 2019
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