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Your search keyword '"Broude NE"' showing total 4 results
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4 results on '"Broude NE"'

1. [Primary structure of the alpha-subunit of Na+,K+-ATPase from the swine kidney. III. Complete nucleotide sequence corresponding to the structural region of the gene].

Author

Monastyrskaia GS, Broude NE, Melkov AM, Smirnov IuV, Malyshev IV, Ahsenyan SG, Salomatina IS, Svehdlov VE, Grishin AV, Petrukhin KE, and Modyanov NN

Subjects
Amino Acid Sequence, Animals, Base Sequence, Swine, Genes, Kidney enzymology, Sodium-Potassium-Exchanging ATPase genetics
Abstract

The nucleotide sequence of the cDNA, containing coding region of the alpha-subunit of the pig kidney Na+, K+-ATPase, was determined. The region contains 3063 b.p. coding for 1021 amino acid residues. In the course of processing, five amino acid residues are cleaved to yield the mature Na+, K+-ATPase alpha-subunit containing 1016 amino acid residues.

Published
1987

2. [Primary structure of the beta-subunit of Na+,K+-ATPase from the swine kidney. II. Reverse transcription, cloning of mRNA, complete nucleotide sequence corresponding to the structural region of the gene].

Author

Broude NE, Monastyrskaia GS, Petrukhin KE, Grishin AV, and Kiiatkin NI

Subjects
Amino Acid Sequence, Animals, Base Sequence, DNA genetics, Hydrolysis, Nucleic Acid Hybridization, Swine, Templates, Genetic, Trypsin, Genes, Kidney enzymology, RNA, Messenger genetics, RNA-Directed DNA Polymerase genetics, Sodium-Potassium-Exchanging ATPase genetics
Abstract

mRNA coding for beta-subunit of Na+, K+-ATPase from pig kidney is about 24-25S as deduced from the hybridization pattern of poly (A)+-RNA with two synthetic oligonucleotides structurally corresponding to two peptides isolated from the tryptic hydrolyzate of beta-subunit. Cloning of cDNA allowed to determine the complete structure of the gene and to deduce the amino acid sequence of beta-subunit of Na+, K+-ATPase. The beta-subunit contains 302 amino acid residues and the protein is not processed at the N-nor at the C-terminus.

Published
1987

3. [Problems associated with the use of highly degenerated oligonucleotide probes. Identification of mRNA and cDNA clones corresponding to the gene for the alpha subunit of Na+,K+-ATPase].

Author

Petrukhin KE, Grishin AV, Arsenian SG, Broude NE, and Grinkevich VA

Subjects
Amino Acid Sequence, Animals, Base Sequence, Chromatography, High Pressure Liquid, Cloning, Molecular, Kidney Medulla enzymology, Nucleic Acid Hybridization, Peptides genetics, Sodium-Potassium-Exchanging ATPase analysis, Swine, DNA genetics, Genes, Oligonucleotides genetics, RNA, Messenger genetics, Sodium-Potassium-Exchanging ATPase genetics
Abstract

Oligonucleotides deduced from the amino acid sequence of a hexapeptide Lys-Asp-Phe-Ala-Glu-Asn were synthesized and used as probes to screen a pig kidney cDNA library for a specific DNA sequence coding for the alpha-subunit of Na+, K+-ATPase. It was shown that the mixed oligoprobe, consisting of 64 heptadecamers, could be only suitable for mRNA blot analysis. To identify the clones with specific cDNA inserts, mixed oligoprobes were fractionated by HPLC technique. For the same purpose a new set of oligonucleotides, synthesized as four groups of 16 different heptadecamers each, was used.

Published
1985

4. [Primary structure of the alpha subunit of Na+,K+-ATPase. II. Isolation, reverse transcription and cloning of messenger RNA].

Author

Petrukhin KE, Broude NE, Arsenian SG, Grishin AV, and Dzhandzhurgazian KN

Subjects
Amino Acid Sequence, Animals, DNA biosynthesis, DNA genetics, Kidney Medulla enzymology, Nucleic Acid Hybridization, RNA, Messenger genetics, Sodium-Potassium-Exchanging ATPase analysis, Swine, Xenopus laevis, Cloning, Molecular, Protein Biosynthesis, RNA, Messenger isolation & purification, RNA-Directed DNA Polymerase genetics, Sodium-Potassium-Exchanging ATPase genetics
Abstract

Messenger RNA, coding for the alpha-subunit of the Na+, K+-ATPase, was isolated from outer medulla of pig kidney. Within 25S-26S region the mRNA yields a band of specific hybridization with three oligonucleotide probes synthesized according to data on structures of three peptides isolated from the tryptic hydrolysate of the protein. Translation of the enriched poly(A+)-fraction of RNA in Xenopus laevis oocytes followed by the immunochemical identification of the products confirmed the presence of RNA coding for the desired protein. This RNA preparation was used for synthesis and cloning of double stranded cDNA.

Published
1985

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