1. [DNA synthesis in the process of rat spermatogenesis].
- Author
-
Glazkov MV and Mednikov BM
- Subjects
- Animals, Base Sequence, DNA isolation & purification, Kinetics, Male, Nucleic Acid Hybridization, Rats, Rats, Inbred Strains, Spermatids metabolism, Spermatogonia metabolism, Thymidine metabolism, DNA biosynthesis, Spermatogenesis
- Abstract
The rat spermatogonia were fractionated in STA-PUT system within 24 hrs following 3H-thymidine injection. DNA isolated from the cells at certain stages of spermatogenesis (spermatogonia, leptotene-zygotene, pachytene, spermatids-spermatozoa) was studied by the method of reassociation kinetics and ultracentrofugation in CsCl gradient. A selective 3H-thymidine incorporation in the region of midrepetitious nucleotide sequences was found at all stages under study, except spermatids-spermatozoa. DNA synthesized during spermatogenesis forms in CsCl density gradient several "satellite" peaks in the "heavy" and "light" gradient zones.
- Published
- 1981