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Your search keyword '"P Kaválková"' showing total 2 results
Start Over Author "P Kaválková" Publisher north holland publishing
2 results on '"P Kaválková"'

1. Urinary metabolomic profiling in mice with diet-induced obesity and type 2 diabetes mellitus after treatment with metformin, vildagliptin and their combination.

Author

Pelantová H, Bugáňová M, Holubová M, Šedivá B, Zemenová J, Sýkora D, Kaválková P, Haluzík M, Železná B, Maletínská L, Kuneš J, and Kuzma M

Subjects
Adamantane pharmacology, Animals, Diabetes Mellitus, Type 2 urine, Diet, Glucose Tolerance Test methods, Hypoglycemic Agents pharmacology, Male, Metabolomics methods, Mice, Mice, Inbred C57BL, Obesity urine, Pyridones metabolism, Vildagliptin, beta-Alanine analogs & derivatives, beta-Alanine metabolism, Adamantane analogs & derivatives, Diabetes Mellitus, Type 2 drug therapy, Diabetes Mellitus, Type 2 metabolism, Metformin pharmacology, Nitriles pharmacology, Obesity drug therapy, Obesity metabolism, Pyrrolidines pharmacology
Abstract

Metformin, vildagliptin and their combination are widely used for the treatment of diabetes, but little is known about the metabolic responses to these treatments. In the present study, NMR-based metabolomics was applied to detect changes in the urinary metabolomic profile of a mouse model of diet-induced obesity in response to these treatments. Additionally, standard biochemical parameters and the expression of enzymes involved in glucose and fat metabolism were monitored. Significant correlations were observed between several metabolites (e.g., N-carbamoyl-β-alanine, N1-methyl-4-pyridone-3-carboxamide, N1-methyl-2-pyridone-5-carboxamide, glucose, 3-indoxyl sulfate, dimethylglycine and several acylglycines) and the area under the curve of glucose concentrations during the oral glucose tolerance test. The present study is the first to present N-carbamoyl-β-alanine as a potential marker of type 2 diabetes mellitus and consequently to demonstrate the efficacies of the applied antidiabetic interventions. Moreover, the elevated acetate level observed after vildagliptin administration might reflect increased fatty acid oxidation., (Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)

Published
2016
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2. Laparoscopic sleeve gastrectomy ameliorates mRNA expression of inflammation-related genes in subcutaneous adipose tissue but not in peripheral monocytes of obese patients.

Author

Trachta P, Dostálová I, Haluzíková D, Kasalický M, Kaválková P, Drápalová J, Urbanová M, Lacinová Z, Mráz M, and Haluzík M

Subjects
Adult, Chemokines, CC genetics, Chemokines, CC metabolism, Female, Gene Expression Profiling, Humans, Inflammation genetics, Inflammation metabolism, Inflammation pathology, Inflammation surgery, Interleukin-10 genetics, Interleukin-10 metabolism, Interleukin-18 genetics, Interleukin-18 metabolism, Laparoscopy, Middle Aged, Monocytes pathology, Obesity genetics, Obesity metabolism, Obesity pathology, Organ Specificity, RNA, Messenger metabolism, Receptors, CCR genetics, Receptors, CCR metabolism, Subcutaneous Fat pathology, Toll-Like Receptor 2 genetics, Toll-Like Receptor 2 metabolism, Toll-Like Receptor 4 genetics, Toll-Like Receptor 4 metabolism, Weight Loss, Gastrectomy methods, Gene Expression, Monocytes metabolism, Obesity surgery, RNA, Messenger genetics, Subcutaneous Fat metabolism
Abstract

Low-grade inflammation links obesity, insulin resistance, and cardiovascular diseases. We investigated the effects of laparoscopic sleeve gastrectomy (LSG) on expression profile of genes involved in inflammatory pathways in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM). At baseline, obese group had significantly increased mRNA expression of proinflammatory chemokines (CCL-3, -17, -22), chemokine receptor CCR1 and cytokines (IL-10, IL-18) in SCAT and chemokine and other proinflammatory receptors (CCR-1, -2, -3, TLR-2, -4) in PM relative to control group. LSG decreased body weight, improved metabolic profile and reduced mRNA expression of up-regulated chemokine receptors, chemokines and cytokines in SCAT. In contrast, expression profiles in PM were largely unaffected by LSG. We conclude that LSG improved proinflammatory profile in subcutaneous fat but not in peripheral monocytes. The sustained proinflammatory and chemotactic profile in PM even 2 years after LSG may contribute to partial persistence of metabolic complications in obese patients after metabolic surgery., (Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.)

Published
2014
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