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For new hybrid rapeseed (Brassica napus L.) identification, previous 33 pairs of SSR markers were used to identified 12 breeding lines and commercial cultivars. Results showed that 3 patterns of SSR markers types with 4-22 distinct markers were obtained from the samples. They were identical type, suspected identical type and distinct type. Among the 12 materials, 3 half-sib hybrids with the same paternal parent exhibited 4-16 distinct markers, and 4 half-sib hybrids with same maternal parent exhibited 7-18 distinct markers. Furthermore, among the commercial hybrids from market, 12-20 distinct markers were found. 0-2 distinct markers occurred between 2 samples with the same hybrid. Therefore, for identifying genuineness of rapeseed hybrids by using 33 SSR markers, the sample could be recognized as the same hybrid or very similar hybrid if the distinct markers were less than 2. That would be the threshold for identifying as different hybrids. The feasibility of this method was further proved by blind identification. [ABSTRACT FROM AUTHOR]