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This paper presents an evaluation of optical fibre sensors for use in brachytherapy treatment where the sensor tip is embedded with radiosensitive scintillation powder, Terbium doped Gadolinium Oxysulphide (Gd2O2S:Tb). Two sensors, fabricated using the same fiber design parameter and size, have been fabricated and evaluated using a Standard Imaging HDR 1000 Well Chamber for increasing numbers of brachytherapy seeds. A sensor holder was designed and 3-D printed to accommodate both the seeds and the sensor for this evaluation purposes. The results show a linear response with number of seeds and demonstrate an excellent agreement with the seed activity measured using a commercial type well chamber. In future, this can be a potential calibration methods for optical fibre sensors prior to use in brachytherapy.