1. Arduino Due based tool to facilitate in vivo two-photon excitation microscopy
- Author
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Stefano Luin, Silvia Landi, Pietro Artoni, Gian Michele Ratto, Sebastian Sulis Sato, Artoni, Pietro, Landi, Silvia, Sato, Sebastian Suli, Luin, Stefano, and Ratto, GIAN MICHELE
- Subjects
0301 basic medicine ,Materials science ,(190.0190) Nonlinear optic ,LOW-COST ,MICROCONTROLLER ,TISSUES ,SENSOR ,SYSTEM ,PROBE ,IONS ,Physics::Optics ,Article ,law.invention ,Settore FIS/03 - Fisica della Materia ,03 medical and health sciences ,0302 clinical medicine ,Quality (physics) ,Optics ,Two-photon excitation microscopy ,law ,Microscopy ,Absorption (electromagnetic radiation) ,(110.0113) Imaging through turbid media ,Scattering ,business.industry ,(110.0110) Imaging system ,Laser ,(180.0180) Microscopy ,Fluorescence ,Atomic and Molecular Physics, and Optics ,030104 developmental biology ,(300.0300) Spectroscopy ,business ,030217 neurology & neurosurgery ,Excitation ,Biotechnology - Abstract
Two-photon excitation spectroscopy is a powerful technique for the characterization of the optical properties of genetically encoded and synthetic fluorescent molecules. Excitation spectroscopy requires tuning the wavelength of the Ti: sapphire laser while carefully monitoring the delivered power. To assist laser tuning and the control of delivered power, we developed an Arduino Due based tool for the automatic acquisition of high quality spectra. This tool is portable, fast, affordable and precise. It allowed studying the impact of scattering and of blood absorption on two-photon excitation light. In this way, we determined the wavelength-dependent deformation of excitation spectra occurring in deep tissues in vivo. (C) 2016 Optical Society of America
- Published
- 2016
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