1. The Presence of Foxp3 Expressing T Cells Within Grafts of Tolerant Human Liver Transplant Recipients
- Author
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Xiangdong Zhao, Kathryn J. Wood, Hironori Haga, Koichi Tanaka, Takaaki Koshiba, Shimon Sakaguchi, Y Li, Tatsuaki Tsuruyama, Donghua Cheng, and Shinji Uemoto
- Subjects
Male ,Pathology ,medicine.medical_specialty ,CD8 Antigens ,medicine.medical_treatment ,Fluorescent Antibody Technique ,Biology ,Polymerase Chain Reaction ,T-Lymphocytes, Regulatory ,law.invention ,law ,medicine ,Humans ,RNA, Messenger ,Polymerase chain reaction ,Transplantation ,Messenger RNA ,Liver Diseases ,Infant ,FOXP3 ,Forkhead Transcription Factors ,Immunosuppression ,Immunohistochemistry ,Molecular biology ,Liver Transplantation ,Staining ,Child, Preschool ,CD4 Antigens ,Female ,Transplantation Tolerance ,CD8 - Abstract
Background. Some experimental transplant-tolerance models have shown that the presence of regulatory T cells within grafts is important for the development of tolerance (Tol). , Methods. To determine if the presence of regulatory T cells correlates with graft acceptance in living-donor liver-transplantation tolerance, the expression of Foxp3 mRNA and the presence of CD4 + , CDB + , and Foxp3 + cells were quantified in biopsies from tolerant recipients by real-time polymerase chain reaction and by immunohistochemistry and immunofluorescent staining (Gr-Tol). The results were compared with biopsies from the recipients on maintenance immunosuppression (Gr-IS), grafts removed because of chronic rejection (Gr-CR), or normal liver (Gr-NL). Results. The expression ofFoxp3 mRNA in Gr-Tol was higher than that in Gr-IS (P=0.07) and Gr-NL (P
- Published
- 2008
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