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Your search keyword '"Emmanuel Merieau"' showing total 3 results
Start Over Author "Emmanuel Merieau" Publisher ovid technologies (wolters kluwer health)
3 results on '"Emmanuel Merieau"'

1. Generation of Immunodeficient Rats With Rag1 and Il2rg Gene Deletions and Human Tissue Grafting Models

Author

Jean-Paul Concordet, Carine Giovannangeli, Véronique Nerrière-Daguin, Frédérique Bellier-Waast, Laurent Tesson, Vanessa Chenouard, Delphine Garnier, Tuan H. Nguyen, Alexandre Fraichard, Séverine Ménoret, Emmanuel Merieau, Ignacio Anegon, Lucas Brusselle, Séverine Remy, Franck Duteille, Frédéric Delbos, Laure-Hélène Ouisse, Claire Usal, Giovannangeli, Carine, Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Genetic and Cellular Engineering in Immunology and Regenerative Medicine (Team 2 - U1064 Inserm - CRTI), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Structure et Instabilité des Génomes (STRING), Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de transplantation urologie-néphrologie (ITUN), Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Chirurgie Plastique Reconstructrice et Esthétique [CHU Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes), and genOway, Lyon

Subjects
Male, 0301 basic medicine, Genotype, Rodent, [SDV]Life Sciences [q-bio], Transplantation, Heterologous, Transplants, Heterologous, medicine.disease_cause, Recombination-activating gene, Animals, Genetically Modified, Rats, Sprague-Dawley, 03 medical and health sciences, Immune system, biology.animal, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, medicine, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Crosses, Genetic, ComputingMilieux_MISCELLANEOUS, Homeodomain Proteins, Transplantation, Mutation, biology, Exons, Skin Transplantation, Phenotype, Rats, 3. Good health, Genetically modified organism, [SDV] Life Sciences [q-bio], Disease Models, Animal, 030104 developmental biology, Liver, Immune System, Immunology, Hepatocytes, Female, Gene Deletion, Interleukin Receptor Common gamma Subunit
Abstract

Background Immunodeficient mice are invaluable tools to analyze the long-term effects of potentially immunogenic molecules in the absence of adaptive immune responses. Nevertheless, there are models and experimental situations that would beneficiate of larger immunodeficient recipients. Rats are ideally suited to perform experiments in which larger size is needed and are still a small animal model suitable for rodent facilities. Additionally, rats reproduce certain human diseases better than mice, such as ankylosing spondylitis and Duchenne disease, and these disease models would greatly benefit from immunodeficient rats to test different immunogenic treatments. Methods We describe the generation of Il2rg-deficient rats and their crossing with previously described Rag1-deficient rats to generate double-mutant RRG animals. Results As compared with Rag1-deficient rats, Il2rg-deficient rats were more immunodeficient because they partially lacked not only T and B cells but also NK cells. RRG animals showed a more profound immunossuppressed phenotype because they displayed undetectable levels of T, B, and NK cells. Similarly, all immunoglobulin isotypes in sera were decreased in Rag1- or Il2rg-deficient rats and undetectable in Rats Rag1 and Il2rg (RRG) animals. Rag1- or Il2rg-deficient rats rejected allogeneic skin transplants and human tumors, whereas animals not only accepted allogeneic rat skin but also xenogeneic human tumors, skin, and hepatocytes. Immune humanization of RRG animals was unsuccessful. Conclusions Thus, immunodeficient RRG animals are useful recipients for long-term studies in which immune responses could be an obstacle, including tissue humanization of different tissues.

Published
2018
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2. Comparative Study of the Immunoregulatory Capacity of In Vitro Generated Tolerogenic Dendritic Cells, Suppressor Macrophages, and Myeloid-Derived Suppressor Cells

Author

Régis Josien, Cédric Louvet, Laura Carretero-Iglesia, Elise Chiffoleau, Laurence Bouchet-Delbos, Emmanuel Merieau, Lucile Drujont, Marcelo Hill, Maria-Cristina Cuturi, Mercedes Segovia, Aurélie Moreau, Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Institut de transplantation urologie-néphrologie (ITUN), Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Faculté de Médecine - Université de Nantes, Fondation Progreffe, IMBIO-DC, The ONE Study (FP7-260687)., ANR: ANR-10IBHU-005, ANR: ANR-11-LABX-0016-01, European Project, Le Bihan, Sylvie, Instituts Hospitalo-Universitaires B - Centre Européen des Sciences de la Transplantation et de l'Immunothérapie (TSI-IHU) - - CESTI (TSI-IHU)2010 - ANR-10-IBHU-0005 - IBHU - VALID, Laboratoires d'excellence - Immunothérapies Grand Ouest - - IGO2011 - ANR-11-LABX-0016 - LABX - VALID, BIODRIM (FP7-305147) European Union 7th Framework Programs - INCOMING, ANR-10-IBHU-0005,CESTI (TSI-IHU),Centre Européen des Sciences de la Transplantation et de l'Immunothérapie (TSI-IHU)(2010), and ANR-11-LABX-0016,IGO,Immunothérapies Grand Ouest(2011)

Subjects
Male, 0301 basic medicine, Adoptive cell transfer, Myeloid, T-Lymphocytes, Cell, Lymphocyte Activation, law.invention, Mice, 03 medical and health sciences, 0302 clinical medicine, law, medicine, Animals, Autoimmune disease, Mice, Inbred BALB C, Transplantation, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, business.industry, Macrophages, Myeloid-Derived Suppressor Cells, Graft Survival, Dendritic Cells, medicine.disease, Adoptive Transfer, 3. Good health, Transplant rejection, Mice, Inbred C57BL, 030104 developmental biology, medicine.anatomical_structure, Immunology, Myeloid-derived Suppressor Cell, Suppressor, Bone marrow, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, 030215 immunology
Abstract

International audience; Background. Regulatory myeloid cell (RMC) therapy is a promising strategy for the treatment of immunological disorders such as autoimmune disease and allograft transplant rejection. Various RMC subsets can be derived from total bone marrow using different protocols, but their phenotypes often overlap, raising questions about whether they are truly distinct.Methods. In this study, we directly compared the phenotype and function of 3 types of RMCs, tolerogenic dendritic cells, suppressor macrophages, and myeloid-derived suppressor cells, generated in vitro from the same mouse strain in a single laboratory.Results. We show that the 3 RMC subsets tested in this study share some phenotypic markers, suppress T cell proliferation in vitro and were all able to prolong allograft survival in a model of skin transplantation. However, our results highlight distinct mechanisms of action that are specific to each cell population.Conclusions. This study shows for the first time a side-by-side comparison of 3 types of RMCs using the same phenotypic and functional assays, thus providing a robust analysis of their similarities and differences.

Published
2016
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