6 results on '"Johan Permert"'
Search Results
2. Impaired Insulin Action on Phosphatidylinositol 3-Kinase Activity and Glucose Transport in Skeletal Muscle of Pancreatic Cancer Patients
- Author
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Markus W. Büchler, Margery K. Herrington, Feng Wang, Helmut Friess, Juleen R. Zierath, Jörgen Larsson, Harriet Wallberg-Henriksson, Bengt Isaksson, Lisa Strömmer, and Johan Permert
- Subjects
Male ,medicine.medical_specialty ,Monosaccharide Transport Proteins ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Blotting, Western ,Muscle Proteins ,In Vitro Techniques ,Phosphatidylinositol 3-Kinases ,Endocrinology ,Insulin resistance ,Internal medicine ,Internal Medicine ,medicine ,Humans ,Insulin ,Kinase activity ,Muscle, Skeletal ,Pancreatic hormone ,Aged ,Glucose tolerance test ,Glucose Transporter Type 4 ,Hepatology ,biology ,medicine.diagnostic_test ,Glucose transporter ,Biological Transport ,Glucose Tolerance Test ,Middle Aged ,medicine.disease ,Pancreatic Neoplasms ,Glucose ,Basal (medicine) ,biology.protein ,3-O-Methylglucose ,Female ,GLUT4 - Abstract
Introduction: Glucose intolerance or overt diabetes occurs in 80% of patients with pancreatic cancer (PC). This associated metabolic disorder includes peripheral insulin resistance, which may be caused by factors produced by the PC. The mechanism underlying PC-associated insulin resistance has not been clearly defined. Aim: To characterize basal and insulin-stimulated glucose transport, phosphatidylinositol (PI) 3-kinase activity, and glucose transporter 4 (GLUT4) in skeletal muscles of PC patients. Methodology: Skeletal muscle samples were obtained from the abdominal wall of 17 PC patients during surgery. Control muscles were sampled in the same way from 11 donors undergoing abdominal surgery for benign diseases. PI 3-kinase activity, glucose transport, and GLUT4 were assessed in vitro in these muscles. Results: In the presence of physiologic concentrations of insulin, glucose transport and PI 3-kinase activity were significantly decreased in the PC group compared with controls. At supraphysiologic insulin concentrations, glucose transport was significantly decreased but PI 3-kinase activity was normalized. In the absence of insulin, these parameters were not significantly different between PC and control groups. Muscle GLUT4 contents were similar between PC and control groups. Conclusion: Defects in insulin-mediated PI 3-kinase activity and glucose transport contribute to the insulin resistance in patients with PC.
- Published
- 2003
3. Physiological Concentrations of Insulin Augment Pancreatic Cancer Cell Proliferation and Glucose Utilization By Activating MAP Kinase, PI3 Kinase and Enhancing GLUT-1 Expression
- Author
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Thomas E. Adrian, Leon Murphy, Drew M. Fehsenfeld, Xian-Zhong Ding, and Johan Permert
- Subjects
medicine.medical_specialty ,Pancreatic disease ,Monosaccharide Transport Proteins ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Glucose uptake ,Phosphatidylinositol 3-Kinases ,Endocrinology ,Internal medicine ,Tumor Cells, Cultured ,Internal Medicine ,medicine ,Humans ,Insulin ,Enzyme Inhibitors ,Pancreatic hormone ,Phosphoinositide-3 Kinase Inhibitors ,Flavonoids ,Glucose Transporter Type 1 ,Hepatology ,biology ,Glucose transporter ,DNA, Neoplasm ,Glucagon ,medicine.disease ,Insulin oscillation ,Androstadienes ,Enzyme Activation ,Pancreatic Neoplasms ,Insulin receptor ,Glucose ,medicine.anatomical_structure ,biology.protein ,Mitogen-Activated Protein Kinases ,Somatostatin ,Wortmannin ,Pancreas ,Protein Kinases ,Cell Division - Abstract
Pancreatic carcinoma is characterized by poor prognosis and lack of response to conventional therapy for reasons that are not clear. Because of the structural relationship between the exocrine and endocrine pancreas and high concentrations of islet hormones bathing pancreatic tissue, we hypothesized that pancreatic cancer cell proliferation and glucose utilization are regulated by pancreatic islet hormones, particularly insulin. Based on this, the effect of islet hormones on pancreatic cancer cells in vitro was investigated. Five pancreatic cancer cell lines, CD11, CD18, HPAF, PANC-1, and MiaPaCa2 were used to investigate the effect of islet hormones on cell proliferation, glucose utilization, and GLUT-1 expression. Insulin, but not somatostatin and glucagon, induced pancreatic cancer cell growth in a concentration- and time-dependent manner. At concentrations within the range of those in the intrapancreatic vasculature, insulin (10(-10)-10(-8) mol/L) markedly increased [3H]-thymidine incorporation. Insulin significantly enhanced glucose utilization of pancreatic cancer cells before it enhanced cell proliferation. The MAPK kinase inhibitor PD 098059 abolished insulin-stimulated DNA synthesis and partially reduced insulin-stimulated glucose uptake. In contrast, the PI3 kinase inhibitor wortmannin substantially inhibited insulin-induced glucose uptake and partially blocked thymidine incorporation. Furthermore, after 24-hour treatment with insulin, GLUT-I expression in pancreatic cancer cells was markedly increased, indicating that insulin enhances glucose utilization partly through increasing glucose transport. These findings suggest that insulin stimulates proliferation and glucose utilization in pancreatic cancer cells by two distinct pathways. Insulin augments DNA synthesis mainly by MAP kinase activation and glucose uptake mainly by PI3 kinase activation and enhancement of GLUT-I expression. High intrapancreatic concentrations of insulin are likely to play an important role in stimulating pancreatic cancer growth indirectly by increasing substrate availability as well as by direct action as a trophic factor.
- Published
- 2000
4. Islet Hormone Secretion in Pancreatic Cancer Patients with Diabetes
- Author
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Thomas E. Adrian, A.B. Fruin, Jörgen Larsson, Kazuhiko Tatemoto, Johan Permert, H. Von Schenck, and Margery K. Herrington
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Male ,endocrine system ,medicine.medical_specialty ,Pancreatic disease ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Adenocarcinoma ,Glucagon ,Diabetes Complications ,Impaired glucose tolerance ,Islets of Langerhans ,Endocrinology ,Internal medicine ,Pancreatic cancer ,Diabetes mellitus ,Diabetes Mellitus ,Internal Medicine ,medicine ,Humans ,Aged ,Glucose tolerance test ,Hepatology ,medicine.diagnostic_test ,business.industry ,Fasting ,Glucose Tolerance Test ,Middle Aged ,Pancreatic Hormones ,medicine.disease ,Pancreatic Neoplasms ,medicine.anatomical_structure ,Hyperglycemia ,Pancreatectomy ,Glucose Clamp Technique ,Female ,Pancreas ,business - Abstract
The diabetes or impaired glucose tolerance that occurs in most patients with pancreatic cancer is characterized by profound insulin resistance. Recent evidence suggests that the diabetes may result from the presence of the tumor rather than being a predisposing factor to development of the malignancy. Some islet hormones have been shown to exhibit diabetogenic effects. To investigate the potential role of these hormones in the diabetic state associated with pancreatic cancer, we measured islet hormones during fasting in pancreatic cancer patients (n = 30), patients with other malignancies (n = 43), and healthy controls (n = 25). Preoperative pancreatic cancer patients were classified as normal glucose tolerance (NGTT), impaired glucose tolerance (IGTT), non-insulin-requiring diabetes (NIRD), and insulin-requiring diabetes (IRD). Nine pancreatic cancer patients were studied after tumor removal by subtotal pancreatectomy. Some preoperative pancreatic cancer patients (n = 19), postoperative patients (n = 9), and controls (n = 8) were also studied during hyperglycemia and following glucagon injection. Fasting plasma C-peptide was elevated in NIRD pancreatic cancer patients compared to controls. Fasting levels of islet amyloid polypeptide (IAPP), glucagon, and somatostatin were elevated in NIRD and IRD patients. IAPP and glucagon, but not somatostatin, normalized following subtotal pancreatectomy. During hyperglycemia, increases in C-peptide and IAPP were seen only in controls and in NGTT and postoperative pancreatic cancer patients. After glucagon infusion, IAPP levels increased in controls and nondiabetic cancer patients; C-peptide levels increased in controls, nondiabetic patients, and NIRD. Responses of C-peptide and IAPP to glucagon normalized after pancreatectomy. During hyperglycemia, glucagon levels fell in all groups except IGTT patients and a decrease in somatostatin concentrations was seen in controls.
- Published
- 1997
5. Humoral growth factors in plasma and liver tissue during liver regeneration and malnutrition
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S. Skullman, Thomas E. Adrian, Jörgen Larsson, and Johan Permert
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medicine.medical_specialty ,Hepatology ,biology ,business.industry ,Regeneration (biology) ,Growth factor ,medicine.medical_treatment ,Gastroenterology ,Radioimmunoassay ,medicine.disease ,Liver regeneration ,Malnutrition ,Endocrinology ,Epidermal growth factor ,Liver tissue ,Internal medicine ,Insulin-like growth factor 2 ,biology.protein ,Medicine ,business - Abstract
Objective: To investigate the potential of a series of growth factors which may be involved in liver regeneration. Design: The concentrations of epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha) and insulin-like growth factor I (IGF-I) were measured in plasma and liver tissue 48h after liver resection or sham operation combined with malnutrition. Methods: Forty-four rats were randomized to either semistarvation or ordinary food intake; half of each group was partially hepatectomized, and the other half underwent a sham operation and served as controls. Growth factors were measured by radioimmunoassay. Results: Plasma EGF levels were increased following semistarvation and were also increased in plasma and liver tissue after 48 h of liver regeneration
- Published
- 1994
6. UP-REGULATION OF UNCOUPLING PROTEIN 2 IN PANCREATIC ISLETS MAY CONTRIBUTE TO BETA-CELL IMPAIRMENT IN ACUTE EXPERIMENTAL PANCREATITIS
- Author
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Ralf Segersvärd, Feng Wang, Margery K. Herrington, Jörgen Larsson, and Johan Permert
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medicine.medical_specialty ,Hepatology ,business.industry ,Endocrinology, Diabetes and Metabolism ,Pancreatic islets ,medicine.disease ,Endocrinology ,medicine.anatomical_structure ,Downregulation and upregulation ,Internal medicine ,Uncoupling protein 2 ,Internal Medicine ,medicine ,Pancreatitis ,Beta cell ,business - Published
- 2004
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