1. Biological basis of [11C]choline-positron emission tomography in patients with breast cancer
- Author
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Kaiyumars B. Contractor, Katy Hogben, Amarnath Challapalli, Carlo Palmieri, Justin Stebbing, Adil Al-Nahhas, Laura M. Kenny, Jacqueline S. Lewis, Sami Shousha, Quang-Dé Nguyen, Eric O. Aboagye, and R. C. Coombes
- Subjects
Choline kinase ,Imaging biomarker ,medicine.diagnostic_test ,Cell growth ,business.industry ,General Medicine ,medicine.disease ,chemistry.chemical_compound ,18f fluorothymidine ,Breast cancer ,chemistry ,Positron emission tomography ,medicine ,Cancer research ,Choline ,Radiology, Nuclear Medicine and imaging ,In patient ,business - Abstract
OBJECTIVE The biological significance of [¹¹C]choline (CHO) uptake in human tumours is unclear and probably linked to choline kinase-α (CHKα) expression and cell proliferation. We directly compared CHO with [¹⁸F]fluorothymidine (FLT), an imaging biomarker of proliferation, by positron emission tomography (PET) in patients with breast cancer to investigate whether cell proliferation is an important determinant of CHO uptake. Furthermore, we evaluated CHKα and the Ki67-labelling index (LIKi67) in tumour biopsies. METHODS Sequential CHO-PET and FLT-PET within the same imaging session were performed in 21 patients with oestrogen receptor (ER)-positive breast cancer (28 lesions). Average and maximum CHO standardized uptake values (SUV) at 60 min: SUV60,av, and SUV60,max, and the rate constant of net irreversible uptake, Ki, were compared with FLT uptake at 60 min: SUV60,av and SUV60,max. Biopsies were stained for CHKα and LIKi67 in eight cases. RESULTS Tumours were equally visible on CHO-PET and FLT-PET imaging. Tumour CHO-PET strongly correlated with FLT imaging variables (Pearson's r=0.83; P
- Published
- 2011