18 results on '"Thierry Hauet"'
Search Results
2. A Brief Period of Hypothermia Induced by Total Liquid Ventilation Decreases End-Organ Damage and Multiorgan Failure Induced by Aortic Cross-Clamping
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Alain Cariou, Renaud Tissier, Nicolas Mongardon, Alice Hutin, Patrick Bruneval, Matthias Kohlhauer, Bijan Ghaleh, Gilles Dhonneur, Sébastien Giraud, Alain Berdeaux, Caroline Barau, Fanny Lidouren, Philippe Micheau, Bruno Costes, Thierry Hauet, Unité Médicale de Soins Intensifs, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), INSERM U955, équipe 3, Pharmacie-Toxicologie, École nationale vétérinaire - Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-École nationale vétérinaire - Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut Mondor de recherche biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Ischémie Reperfusion en Transplantation d’Organes Mécanismes et Innovations Thérapeutiques ( IRTOMIT), Université de Poitiers-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre hospitalier universitaire de Poitiers (CHU Poitiers), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service d'anatomo-pathologie [CHU HEGP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Département de génie mécanique [Sherbrooke] (UdeS), Université de Sherbrooke (UdeS), Hôpital Cochin [AP-HP], Service de réanimation médicale polyvalente [CHU Cochin], Service d'anesthésie-réanimation SAMU94-SMUR94 [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Poitiers, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)
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Male ,Liquid Ventilation ,Time Factors ,End organ damage ,Multiple Organ Failure ,[SDV]Life Sciences [q-bio] ,030204 cardiovascular system & hematology ,Hypothermia induced ,Constriction ,Random Allocation ,03 medical and health sciences ,0302 clinical medicine ,Hypothermia, Induced ,medicine.artery ,Animals ,Medicine ,Aorta ,ComputingMilieux_MISCELLANEOUS ,business.industry ,030208 emergency & critical care medicine ,medicine.disease ,Multiorgan failure ,Clamping ,Anesthesiology and Pain Medicine ,Anesthesia ,Total Liquid Ventilation ,Rabbits ,business ,Perfusion ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
In animal models, whole-body cooling reduces end-organ injury after cardiac arrest and other hypoperfusion states. The benefits of cooling in humans, however, are uncertain, possibly because detrimental effects of prolonged cooling may offset any potential benefit. Total liquid ventilation (TLV) provides both ultrafast cooling and rewarming. In previous reports, ultrafast cooling with TLV potently reduced neurological injury after experimental cardiac arrest in animals. We hypothesized that a brief period of rapid cooling and rewarming via TLV could also mitigate multiorgan failure (MOF) after ischemia-reperfusion induced by aortic cross-clamping.Anesthetized rabbits were submitted to 30 minutes of supraceliac aortic cross-clamping followed by 300 minutes of reperfusion. They were allocated either to a normothermic procedure with conventional ventilation (control group) or to hypothermic TLV (33°C) before, during, and after cross-clamping (pre-clamp, per-clamp, and post-clamp groups, respectively). In all TLV groups, hypothermia was maintained for 75 minutes and switched to a rewarming mode before resumption to conventional mechanical ventilation. End points included cardiovascular, renal, liver, and inflammatory parameters measured 300 minutes after reperfusion.In the normothermic (control) group, ischemia-reperfusion injury produced evidence of MOF including severe vasoplegia, low cardiac output, acute kidney injury, and liver failure. In the TLV group, we observed gradual improvements in cardiac output in post-clamp, per-clamp, and pre-clamp groups versus control (53 ± 8, 64 ± 12, and 90 ± 24 vs 36 ± 23 mL/min/kg after 300 minutes of reperfusion, respectively). Liver biomarker levels were also lower in pre-clamp and per-clamp groups versus control. However, acute kidney injury was prevented in pre-clamp, and to a limited extent in per-clamp groups, but not in the post-clamp group. For instance, creatinine clearance was 4.8 ± 3.1 and 0.5 ± 0.6 mL/kg/min at the end of the follow-up in pre-clamp versus control animals (P = .0004). Histological examinations of the heart, kidney, liver, and jejunum in TLV and control groups also demonstrated reduced injury with TLV.A brief period of ultrafast cooling with TLV followed by rapid rewarming attenuated biochemical and histological markers of MOF after aortic cross-clamping. Cardiovascular and liver dysfunctions were limited by a brief period of hypothermic TLV, even when started after reperfusion. Conversely, acute kidney injury was limited only when hypothermia was started before reperfusion. Further work is needed to determine the clinical significance of our results and to identify the optimal duration and timing of TLV-induced hypothermia for end-organ protection in hypoperfusion states.
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- 2016
3. Improving Long-Term Outcome in Allograft Transplantation: Role of Ionic Composition and Polyethylene Glycol
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Frédéric Favreau, Thierry Hauet, Benoit Barrou, Raphael Thuillier, Thibault Desurmont, Jean-Michel Goujon, Michel Eugene, Sébastien Giraud, Ischémie - Reperfusion en transplatation rénale, Université de Poitiers-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre hospitalier universitaire de Poitiers (CHU Poitiers), FLIRT, Fédération pour L'étude de l'Ischémie Reperfusion en Transplantation, Université de Poitiers, Université Pierre et Marie Curie - Paris 6 (UPMC), CHU Henri Mondor, Réseau CENTAURE, Conseil General de la Vienne, Region Poitou Charentes, Banque Tarneaud, Poitiers, CHU de Poitiers and Inserm, Societe Francophone de Transplantation, French Foundation of Transplantation, and CHU Henri Mondor [Créteil]
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Male ,Graft outcome ,Necrosis ,Swine ,[SDV]Life Sciences [q-bio] ,medicine.medical_treatment ,TO-MESENCHYMAL TRANSITION ,030232 urology & nephrology ,ISCHEMIA-REPERFUSION INJURY ,Adaptive Immunity ,030230 surgery ,Hydroxyethyl starch ,Polyethylene Glycols ,chemistry.chemical_compound ,0302 clinical medicine ,AUTOTRANSPLANTED PIG KIDNEYS ,SURFACE MODIFICATION ,Hypoxia ,Interstitial fibrosis ,MOLECULAR-MECHANISMS ,RENAL-TRANSPLANTATION ,Immunosuppression ,Kidney preservation ,3. Good health ,Treatment Outcome ,Reperfusion Injury ,medicine.symptom ,medicine.drug ,medicine.medical_specialty ,Organ Preservation Solutions ,Polyethylene glycol ,TUBULAR EPITHELIAL-CELLS ,STEALTH ERYTHROCYTES ,PRESERVATION SOLUTION ,Andrology ,03 medical and health sciences ,In vivo ,PEG ratio ,medicine ,Animals ,Transplantation, Homologous ,Immune response ,Transplantation ,business.industry ,medicine.disease ,Kidney Transplantation ,Immunity, Innate ,Surgery ,chemistry ,48-HOUR COLD-STORAGE ,business ,Reperfusion injury ,Ischemia reperfusion injury - Abstract
International audience; Background. Ischemia reperfusion injury (IRI) is inherent to transplantation, and correlates with negative outcome. Limiting IRI requires new preservation. Fourth generation solutions are emerging, using new colloid based on polyethylene glycol (PEG) polymers and extracellular ionic composition. We evaluated their eventual benefits for optimal resistance to IRI and improved outcome. Methods. Using primary cell culture and a preclinical pig model of low-mismatch kidney allograft transplantation, not requiring immunosuppression, we compared the following solutions: UW (University of Wisconsin), high potassium with hydroxyethyl starch, gold standard in preservation; IGL-1 (Institute George Lopez-1), low potassium solution using PEG (35 kDa, 1 g/L); and SCOT (Solution de Conservation des Organes et Tissus), plasma-like ionic composition, containing PEG 20 kDa (30 g/L). Results. In vitro, SCOT-preserved cells had better viability and less necrosis. In vivo, SCOT-grafts had better function recovery, with limited histological injury compared with the other solutions. During the 3 months follow-up, we found low innate and adaptative immune response in SCOT organs, whereas other groups presented high rate of invasion and antigen presentation. SCOT-preserved kidneys showed low fibrosis, transforming growth factor-beta expression and apoptosis compared with the other groups. These differences impacted survival at 3 months, which was low in UW (20%) and IGL-1 (40%) groups, whereas it remained high for SCOT animals (80%, P < 0.05 to UW). Conclusions. In conclusion, plasma-like ionic composition and nontoxic molecule PEG provide high resistance against IRI and optimize graft outcome. Such solutions could be invaluable for the use of fragile organs, such as from extended criteria or deceased after cardiac death donors.
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- 2011
4. Modulation of Peripheral-Type Benzodiazepine Receptor During Ischemia Reperfusion Injury in a Pig Kidney Model: A New Partner of Leukemia Inhibitory Factor in Tubular Regeneration
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Keqiang Zhang, Gérard Mauco, Michel Carretier, Jerome Cau, Frédéric Favreau, Vassilios Papadopoulos, Jean Michel Goujon, Thibault Desurmont, Thierry Hauet, and Séverine Deretz
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Male ,medicine.medical_specialty ,Proliferation index ,Swine ,Ischemia ,Kidney ,Leukemia Inhibitory Factor ,Internal medicine ,medicine ,Animals ,Regeneration ,RNA, Messenger ,Receptor ,Messenger RNA ,Interleukin-6 ,business.industry ,Proteins ,Receptors, GABA-A ,medicine.disease ,Kidney Tubules ,medicine.anatomical_structure ,Endocrinology ,Mitochondrial permeability transition pore ,Reperfusion Injury ,Surgery ,business ,Reperfusion injury ,Leukemia inhibitory factor - Abstract
Background It was demonstrated that postischemic kidney expresses different factors in a pattern that recapitulates expression of these factors in the developing kidney. We investigated whether peripheral-type benzodiazepine receptor (PBR), which belongs to the mitochondrial permeability transition pore and is essential during development, could be influenced by the ischemia-reperfusion injury process when compared with leukemia inhibitor factor (LIF). Study design PBR, LIF, and LIF receptor messengers and proteins were analyzed in adult normal and ischemic kidney under conditions mimicking cardiac arrest: 18 pigs were studied after 60 minutes of warm ischemia and reperfusion for 7 days and compared with sham-operated (Sham, n=12) and control (CONT, n=12) groups. The same messengers and proteins were assessed in fetal kidneys. Results In normal kidney, PBR was expressed in descending and ascending limbs of Henle and in distal tubules. After ischemia-reperfusion injury, PBR mRNA significantly increased between days 1 and 7 in cortex and outer medulla. PBR protein increased between days 1 and 7, and was transiently expressed in proximal tubules at days 1 and 3 and returned to basal level at day 7. LIF messenger and protein increased rapidly at day 1 in proximal tubules. In turn, LIF receptor messenger and protein were not changed during reperfusion. Conclusions These results suggest that PBR may be implicated in ischemia-reperfusion injury and, particularly, in the regenerative process within proximal tubules with LIF. These new insights open the possibility of novel targets for organ protection and repair.
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- 2006
5. A MODIFIED UNIVERSITY OF WISCONSIN PRESERVATION SOLUTION WITH HIGH-NA+ LOW-K+ CONTENT REDUCES REPERFUSION INJURY OF THE PIG KIDNEY GRAFT1
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Carole Doucet, Zeqiu Han, Aoumeur Hadj Aissa, Thierry Hauet, Silvina Ramella-Virieux, Vassilios Papadopoulos, and Michel Carretier
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Transplantation ,Pathology ,medicine.medical_specialty ,Kidney ,business.industry ,medicine.medical_treatment ,Urology ,Cold storage ,Renal function ,medicine.disease ,Autotransplantation ,medicine.anatomical_structure ,Fibrosis ,medicine ,Viaspan ,business ,Reperfusion injury - Abstract
Background. Ischemia-reperfusion injury has been associated with both early and late effects on allografts in the form of delayed graft function and decreased graft survival. Recent studies demonstrated that functional parameters were influenced by cold storage conditions and particularly the ratio of Na + :K + of the preservation solution. Methods. We have extended this study to examine whether the high-Na + low-K + formulation of Belzer's solution (HEH) was efficient in an autotransplanted pig kidney model when compared with the classical low-Na + high-K + University of Wisconsin solution and the new high-Na + low-K + Celsior solution. Kidneys were harvested, cold flushed, and preserved for 24, 48, or 72 hr with HEH, Celsior solution, or University of Wisconsin solution and autotransplanted. Renal function was determined on days 1, 3, 7, and 14, and at 4 to 16 weeks after autotransplantation. Histologic changes and cell infiltration were assessed on kidney biopsy specimens taken after reperfusion (30-40 min), at days 5 and 14, and at 4 to 5 and 10 to 12 weeks after surgery. Peripheral benzodiazepine receptor (PBR), a structural mitochondrial protein, was also studied. Results. Cold storage in HEH resulted in reduction of delayed graft function and renal damage, with a decrease in interstitial inflammation. HEH reduced interstitial fibrosis, tubular atrophy, and improved PBR expression. Conclusion. This study suggests that cold preservation in HEH has a beneficial action in in vivo renal preservation and reduces tubular necrosis, interstitial inflammation, and fibrosis in these groups. In addition, PBR detection was correlated to the level of preservation integrity.
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- 2003
6. PROTECTION OF AUTOTRANSPLANTED PIG KIDNEYS FROM ISCHEMIA-REPERFUSION INJURY BY POLYETHYLENE GLYCOL1
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Hervé Baumert, Michel Eugene, Catherine Godart, H Gibelin, Thierry Hauet, Jean Michel Goujon, Imed Ben Amor, Michel Carretier, and Alain Vandewalle
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Transplantation ,Kidney ,medicine.medical_specialty ,Fractional excretion of sodium ,urogenital system ,business.industry ,Ischemia ,Urology ,Renal function ,medicine.disease ,Surgery ,medicine.anatomical_structure ,PEG ratio ,medicine ,business ,Reperfusion injury ,Kidney disease - Abstract
Background. Ischemia-reperfusion injury (IRI) is often responsible for graft rejection and leads to delayed graft function of cadaveric kidneys. We have shown that adding polyethylene glycol (PEG 20M) to the preservation solutions helps protect isolated perfused pig kidneys against cold ischemia and reperfusion injury. Methods. We compared the effects of adding PEG to a simplified high-K 1 perfusion solution of cold-stored kidneys to Euro-Collins or University of Wisconsin solutions on the function of reperfused autotransplanted pig kidneys. The left kidney was cold-flushed with the preservation solutions and stored for 48 hr at 4°C before reimplantation. Creatinine clearance and fractional excretion of sodium were analyzed 2 days before surgery and over 7 days after transplantation. Histological sections were obtained 40 min after reperfusion and on day 7 after surgery. Results. Adding PEG to the perfusate significantly reduced IRI from autotransplanted pig kidneys. Creatinine clearance was significantly higher and fractional excretion of sodium was significantly lower in pigs transplanted with kidneys cold-flushed with PEG-supplemented perfusate than in those flushed with Euro-Collins or University of Wisconsin solutions. PEG supplementation also better preserved the integrity of kidney cells and markedly reduced interstitial cell infiltrates. Conclusion. PEG protects against IRI and reduces early cellular inflammation. PEG may impair the recruitment and migration of leukocytes into retransplanted pig kidneys. Cold preservation of donor organs with PEG-supplemented solutions may therefore help limit IRI in human renal transplantation. Delayed graft function (DGF) is a major problem affecting kidney allografts after transplantation. DGF occurs in 30 ‐ 50% of patients given a first cadaver graft and can be exac
- Published
- 2000
7. Trimetazidine Reduces Renal Dysfunction by Limiting the Cold Ischemia/Reperfusion Injury in Autotransplanted Pig Kidneys
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Hervé Baumert, Michel Eugene, Alain Vandewalle, L. Lacoste, Jean-Paul Tillement, Thierry Hauet, Michel Carretier, and Jean-Michel Goujon
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Graft Rejection ,Male ,medicine.medical_specialty ,Swine ,Vasodilator Agents ,Urinary system ,Trimetazidine ,Urology ,Ischemia ,Renal function ,Kidney ,Kidney Function Tests ,Transplantation, Autologous ,Methylamines ,Reference Values ,medicine ,Animals ,business.industry ,Graft Survival ,General Medicine ,medicine.disease ,Kidney Transplantation ,Surgery ,Cold Temperature ,Transplantation ,Disease Models, Animal ,Treatment Outcome ,medicine.anatomical_structure ,Nephrology ,Reperfusion Injury ,business ,Reperfusion injury ,Dimethylamines ,Kidney disease ,medicine.drug - Abstract
Ischemia/reperfusion injury leads to delayed graft function, which is a major problem in kidney transplantation. This study investigated the effects of adding trimetazidine (TMZ) to the perfusate of cold-stored kidneys on the function of reperfused autotransplanted pig kidney. The left kidney was removed and cold-flushed with Euro-Collins (EC), or University of Wisconsin (UW) solutions with or without 10 -6 M TMZ and stored for 48 h at 4°C. The kidneys were then autotransplanted and the contralateral kidneys were removed. Several parameters were analyzed over the 14 d after transplantation. The survival rate was 57% in pigs transplanted with kidneys cold-flushed with UW and 43% for those flushed with EC solution; it was 100% for pigs having kidneys cold-flushed with TMZ-supplemented UW and EC solutions. The functions of the transplanted kidneys were also better preserved after cold flush with TMZ-supplemented solutions than with TMZ-free solutions. Creatinine clearance was higher and the urinary excretion of trimethylamine- N -oxide and dimethylamine, used as markers of renal medulla injury, were lower in animals transplanted with kidneys cold-flushed with TMZ-supplemented solutions than with TMZ-free solutions. The cytoprotective action of TMZ also reduced interstitial and peritubular inflammation and the numbers of infiltrating mononuclear CD45 + and CD3 + T cells. These results indicate that the tissue damage due to ischemia/reperfusion injury may be prevented, at least in part, by adding TMZ to preservation solutions.
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- 2000
8. RENOPROTECTIVE EFFECTS OF TRIMETAZIDINE AGAINST ISCHEMIA-REPERFUSION INJURY AND COLD STORAGE PRESERVATION: A PRELIMINARY STUDY1
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Jean-Paul Tillement, Jean-Pierre Richer, Hervé Baumert, Michel Carretier, Michel Eugene, Jean-Michel Goujon, L. Lacoste, and Thierry Hauet
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Transplantation ,medicine.medical_specialty ,Kidney ,business.industry ,medicine.medical_treatment ,Urology ,Trimetazidine ,Cold storage ,medicine.disease ,Autotransplantation ,Surgery ,medicine.anatomical_structure ,medicine ,business ,Blood urea nitrogen ,Reperfusion injury ,medicine.drug ,Kidney disease - Abstract
Background. Initial ischemia-reperfusion injury is associated with organ retrieval, storage, and transplantation adversely affects early graft function and influences the development of chronic graft dysfunction. We have recently shown that the protective agent trimetazidine (TMZ) added to preservation solutions: Euro-collins (EC) and University of Wisconsin (UW) was efficient to protect kidneys from ischemia-reperfusion injury in an isolated perfused kidney model. We extended these observations to investigate the role of this drug in the development and progression of organ dysfunction in the autotransplant pig kidney model. Methods. Five experimental groups were studied. After 48-hr cold preservation, autotransplantation and immediate controlateral nephrectomy was then performed in group EC (EC+placebo (n=8), EC+TMZ (n=8), UW+placebo (n=7), and (UW+TMZ) (n=7) and compared with control group (uninephrectomized, n=4) during 14 days. Blood and urine samples were collected for the measurement of creatinine and blood urea nitrogen on postoperative days 1, 3, 5, 7, 11, and 14. Histological analysis was performed after reperfusion and at day 14. Results. Survivals were 100% in group B and D versus 42% in group A and 57% in group C. Urine production occurred earlier after autotransplantation from TMZ preserved kidneys than in placebo preserved groups. Peak creat and blood urea nitrogen was significantly greater in groups B and D than in groups A and C. TMZ was also efficient both to reduce ischemia-reperfusion injury and to decrease cellular infiltration. Conclusion. These results support the beneficial effect of TMZ against ischemia-reperfusion injury and its early effects on grafts in the form of delayed graft function and decreased graft survival. In addition, TMZ reduces inflammatory cellular infiltration in the renal parenchyma.
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- 1999
9. TRIMETAZIDINE PREVENTS RENAL INJURY IN THE ISOLATED PERFUSED PIG KIDNEY EXPOSED TO PROLONGED COLD ISCHEMIA1
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D. Mothes, Jean-Michel Goujon, Laurence Le Moyec, Jean-Paul Tillement, Michel Carretier, Michel Eugene, J.C. Caritez, and Thierry Hauet
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Transplantation ,medicine.medical_specialty ,Kidney ,Chemistry ,medicine.medical_treatment ,Trimetazidine ,Ischemia ,Cold storage ,Renal function ,Pharmacology ,medicine.disease ,Surgery ,medicine.anatomical_structure ,medicine ,Saline ,Reperfusion injury ,medicine.drug - Abstract
Background. Ischemia caused by cold storage (CS) and reperfusion of the kidney is often responsible for delayed graft function after transplantation. Significant attention has been focused on the cascade of events involved in ischemia-reperfusion injury, with the objective of identifying drugs to ameliorate the functional damage that occurs. Methods. The purpose of this study was to evaluate the renal function of isolated perfused pig kidneys after 48 hr of CS with Euro-Collins (EC) solution plus trimetazidine (EC+TMZ), standard EC solution, or University of Wisconsin (UW) solution. Normothermic isolated perfused pig kidneys were randomized into five experimental groups: (A) control group (cold flush with cold heparinized saline and immediately reperfused ; n=6); (B) cold flush with cold heparinized saline with TMZ (10 -6 M), n=6; (C) 48 hr of CS with EC and reperfusion (n=8); (D) 48 hr of CS with EC+TMZ alone and reperfusion (n=8); (E) 48 hr of CS with UW and reperfusion (n=8). Proton nuclear magnetic resonance spectroscopy and biochemical studies were performed for the functional evaluation during reperfusion. Lipid peroxidation was also determined. Histological examination (optical and electron microscopy) was performed after CS and reperfusion. Results. Using TMZ, the renal perfusate flow rate as well as the glomerular filtration rate and proximal tubular function were significantly improved. This improvement of renal function during reperfusion was correlated with a less significant cellular and interstitial edema. In addition, tubular injury markers were significantly lower in the group preserved with EC+TMZ, and TMZ reduced lipid peroxidation dramatically during reperfusion. Conclusions. The addition of TMZ to the EC solution increased the preservation quality and renal tubular function, and gave protection from reperfusion injury better than EC alone or UW. These results strongly suggest that TMZ has a cytoprotective effect and may therefore be useful for kidney preservation.
- Published
- 1997
10. Recombinant C1INH Reduces Ischemia Reperfusion-Induced Immune Response and Improves Kidney Graft Outcome
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T. Saintyves, Thierry Hauet, Gilles Blancho, Beatrijs Oortwijn, R. Thuillier, Jérôme Danion, E. van Amersfoort, and S. Le Pape
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Transplantation ,Kidney ,business.industry ,Ischemia ,Pharmacology ,medicine.disease ,Outcome (game theory) ,law.invention ,medicine.anatomical_structure ,Immune system ,law ,medicine ,Recombinant DNA ,business - Published
- 2014
11. Determination of Ischemia Reperfusion Mechanisms at the Cellular Level: The Unfolded Protein Response
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Thierry Hauet, S. Le Pape, and R. Thuillier
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Transplantation ,Chemistry ,Unfolded protein response ,Ischemia ,medicine ,Cellular level ,medicine.disease ,Cell biology - Published
- 2014
12. Organ Preservation Revisited: Anticoagulation Improves Early and Long Term Kidney Graft Function
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Sébastien Giraud, Solenne Tillet, M. Petitou, R. Thuillier, Gérard Mauco, William Hebrard, and Thierry Hauet
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Transplantation ,Kidney ,medicine.medical_specialty ,medicine.anatomical_structure ,business.industry ,medicine ,Intensive care medicine ,business ,Graft function ,Surgery ,Term (time) - Published
- 2012
13. Metabonomic Analysis of Perfusion Solution with NMR: A Potential Tool to Predict Graft Outcome in Deceased after Cardiac Arrest Donor Conditions
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Edouard Baulier, Lionel Badet, J. P. Tillement, Ricardo Codas, William Hebrard, Thierry Hauet, Delphine Bon, Frédéric Favreau, François Seguin, and Nadège Boildieu
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Transplantation ,medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,Cardiology ,business ,Perfusion ,Outcome (game theory) ,Surgery - Published
- 2012
14. CURCUMIN TREATMENT DURING PRESERVATION IMPROVES LONG TERM GRAFT OUTCOME
- Author
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H. Lathelize, J. Paarkkinen, R. Thuillier, Thierry Hauet, and Lauri Vaahtera
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Oncology ,Transplantation ,medicine.medical_specialty ,Curcumin treatment ,business.industry ,Internal medicine ,medicine ,business ,Outcome (game theory) ,Term (time) - Published
- 2010
15. ISCHEMIA REPERFUSION INJURY THERAPY: INHIBITOR OF COAGULATION DURING PRESERVATION RESCUES CHRONIC KIDNEY INFLAMMATION, FIBROSIS AND GRAFT LOSS
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Sébastien Giraud, O. Page, R. Thuillier, and Thierry Hauet
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Transplantation ,Pathology ,medicine.medical_specialty ,Coagulation ,Fibrosis ,business.industry ,Ischemia ,medicine ,Kidney inflammation ,medicine.disease ,Graft loss ,business ,Reperfusion injury - Published
- 2010
16. IMPROVING KIDNEY PRESERVATION: HEMO2LIFE® SUPPLEMENTATION RESCUES ISCHEMIA REPERFUSION INJURY
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Franck Zal, T. M.T. ngo, D. Dutheil, Thierry Hauet, and R. Thuillier
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Transplantation ,medicine.medical_specialty ,Kidney preservation ,business.industry ,Internal medicine ,Ischemia ,Cardiology ,Medicine ,business ,medicine.disease ,Reperfusion injury - Published
- 2010
17. DIAGNOSING ISCHEMIA REPERFUSION INJURY: ANALYSIS OF SEVERITY MARKERS IN A PIG MODEL OF KIDNEY TRANSPLANTATION
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R. Thuillier, Thierry Hauet, Frédéric Favreau, Cedric Nadeau, and Ludivine Rossard
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Transplantation ,medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,Ischemia ,Cardiology ,Pig model ,medicine.disease ,business ,Reperfusion injury ,Kidney transplantation - Published
- 2010
18. IMPROVEMENT OF EURO-COLLINS (EC) SOLUTION BY TRIMETAZIDINE
- Author
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Bertrand Doré, Jean Paul Tillement, Hervé Baumert, Imed Ben Amor, Michel Carretier, Jean-Pierre Faure, Jacques Irani, and Thierry Hauet
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medicine.medical_specialty ,business.industry ,Urology ,Internal medicine ,Cardiology ,Trimetazidine ,Medicine ,Viaspan ,business ,medicine.drug - Published
- 1999
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