1. In situ hybridization in paracoccidioidomycosis.
- Author
-
De Brito T, Sandhu GS, Kline BC, Aleff RA, Sandoval MP, Santos RT, Brandão AA, and Lacaz CS
- Subjects
- Adult, Animals, Guinea Pigs, Humans, Male, Middle Aged, Paracoccidioidomycosis pathology, In Situ Hybridization methods, Paracoccidioides genetics, Paracoccidioidomycosis microbiology
- Abstract
In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.
- Published
- 1999