1. Regulation of the transcription factor E2F1 mRNA in ovarian granulosa cells of cattle
- Author
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Excel Rio S. Maylem, B.C. Morrell, Leon J. Spicer, Luis F. Schutz, M. Chiara Perego, and Lingna Zhang
- Subjects
endocrine system ,Granulosa cell ,medicine.medical_treatment ,Andrology ,03 medical and health sciences ,Follicle ,Ovarian Follicle ,Follicular phase ,Gene expression ,Genetics ,medicine ,Animals ,RNA, Messenger ,Ovarian follicle ,030304 developmental biology ,Cell Proliferation ,0303 health sciences ,Granulosa Cells ,Estradiol ,Chemistry ,Growth factor ,Reproduction ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,Cell cycle ,040201 dairy & animal science ,stomatognathic diseases ,medicine.anatomical_structure ,Gene Expression Regulation ,Theca ,Theca Cells ,Animal Science and Zoology ,Cattle ,Female ,biological phenomena, cell phenomena, and immunity ,Follicle Stimulating Hormone ,E2F1 Transcription Factor ,Food Science - Abstract
The E2F family of transcription factors plays an important role in the control of the cell cycle, cell proliferation, and differentiation, and their role in ovarian function is just emerging. Although some evidence suggests a possible role of E2F1 in ovarian follicular development, what regulates its production in ovarian cells is unknown. Objectives of this study were to determine whether: (i) E2F1 gene expression in granulosa cells (GCs) and theca cells (TCs) change with follicular development and (ii) E2F1 mRNA abundance in TC and GC is hormonally regulated. Using real-time PCR, E2F1 mRNA abundance in GC was 5.5-fold greater (P 8 mm) follicles, but in TC, E2F1 expression did not differ among follicle sizes. SM-follicle GC had 2.1-fold greater (P 0.10) abundance of E2F1 mRNA in LG-follicle TC or GC. Follicle-stimulating hormone (FSH) had no effect (P > 0.10) on E2F1 gene expression in SM- or LG-follicle GC. SM-follicle GC were concomitantly treated with insulin-like growth factor 1 (30 ng/mL), FSH (30 ng/mL), and either 0 or 30 ng/mL of FGF9 with or without 50 µM of an E2F inhibitor (E2Fi; HLM0064741); FGF9 alone increased (P
- Published
- 2019