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Your search keyword '"Shi, Liuzhi"' showing total 2 results
Start Over Author "Shi, Liuzhi" Publisher oxford university press
2 results on '"Shi, Liuzhi"'

1. Gab2 promotes acute myeloid leukemia growth and migration through the SHP2-Erk-CREB signaling pathway.

Author

Gong R, Li H, Liu Y, Wang Y, Ge L, Shi L, Wu G, Lyu J, Gu H, and He L

Subjects
Adaptor Proteins, Signal Transducing, Aged, Humans, Matrix Metalloproteinase 9, RNA, Small Interfering genetics, Signal Transduction, Leukemia, Myeloid, Acute genetics, Matrix Metalloproteinase 2
Abstract

Acute myeloid leukemia (AML) is a hematologic malignant disease largely affecting older adults with poor outcomes. Lack of effective targeted treatment is a major challenge in managing the disease in the clinic. Scaffolding adaptor Gab2 is amplified in a subset of AML. However, the causative role of Gab2 in AML remains to be explored. In this study, it was found that Gab2 was expressed at high levels in AML patient samples and AML cell lines. Experiments by knocking down Gab2 expression using shRNA showed that Gab2 promoted AML cell growth and migration in vitro and in vivo. Further studies using Gab2 mutants and pharmacological inhibitors revealed that Gab2 increased CREB phosphorylation via the SHP-2/Erk signaling pathway. CREB phosphorylation contributed to Gab2-induced cell migration by increasing MMP2 and MMP9 expression. This research indicates that high Gab2 expression promotes AML progression through the SHP2-Erk-CREB signaling pathway. CREB suppression may help treat AML with high Gab2 expression., (©2022 Society for Leukocyte Biology.)

Published
2022
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2. Primaquine phosphate induces the apoptosis of ATRA-resistant acute promyelocytic leukemia cells by inhibition of the NF-κB pathway.

Author

Ma L, Chen L, Li H, Ge L, Wang S, Zhang Z, Huang H, Shi L, Li T, Gu H, Lyu J, and He L

Subjects
Adult, Animals, Cell Line, Tumor, Cell Proliferation drug effects, Cell Survival drug effects, Female, Humans, Male, Mice, Inbred BALB C, Mice, Nude, Middle Aged, Primaquine chemistry, Tumor Stem Cell Assay, Xenograft Model Antitumor Assays, Apoptosis drug effects, Drug Resistance, Neoplasm drug effects, Leukemia, Promyelocytic, Acute pathology, NF-kappa B metabolism, Primaquine pharmacology, Signal Transduction drug effects, Tretinoin pharmacology
Abstract

As a subtype of acute myeloid leukemia (AML), acute promyelocytic leukemia (APL) is characterized by a chromosomal translocation, most of which result in the production of a PML-RAR alpha fusion protein. Although the overall survival rate of APL patients has improved dramatically due to all-trans retinoic acid (ATRA) treatment, ATRA-resistance remains a clinical challenge in the management of APL. Therefore, alternative agents should be considered for ATRA-resistant APL patients. Here, we report that antimalaria drug primaquine phosphate (PRQ) exhibits an anti-leukemia effect on both ATRA-sensitive cell line NB4 and ATRA-resistant APL cell lines, NB4-LR2, NB4-LR1, and NB4-MR2. Moreover, PRQ significantly inhibited primary colony formation of untreated or relapsed APL patients. Further study showed that PRQ could induce the apoptosis of APL cells by inhibiting NF-κB signaling pathway. The in vivo study showed that PRQ significantly inhibited NB4-LR2 xenograft tumors growth. These results suggest that PRQ is a potential therapeutic agent for ATRA-resistant APL patients., (©2020 Society for Leukocyte Biology.)

Published
2020
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